Population genetic of the Indonesian rosewood (<i>Dalbergia latifolia</i>) from Java and West Nusa Tenggara revealed using sequence-related amplified polymorphism

Yulita, Kusumadewi Sri; Susila,; Rachmat, Henti Hendalastuti; Dwiyanti, Fifi Gus; Atikah, Tika Dewi; Subiakto, Atok; Pratama, Bayu Indra; Setyawati, Titiek; Wardani, Wita; Fambayun, Rizki Ary; Arrofaha, Nawwall; Kamal, Irsyad

doi:10.1080/21580103.2022.2123051

Cited by 4 publications

(4 citation statements)

References 46 publications

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“… Terrestrial [ 23 ] 03 Streptophyta Fabales Fabaceae Dalbergia sp. Terrestrial [ 24 ] 04 Streptophyta Sapindales Sapindaceae Lepisanthes sp. Secondary rainforest [ 25 ] 05 Streptophyta Sapindales Anacardiaceae Mangifera sp.…”

Section: Resultsmentioning

confidence: 99%

Using pollen DNA metabarcoding to trace the geographical and botanical origin of honey from Karangasem, Indonesia

ullah,

Huyop,

Wahab

et al. 2024

Heliyon

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Section: Resultsmentioning

confidence: 99%

Using pollen DNA metabarcoding to trace the geographical and botanical origin of honey from Karangasem, Indonesia

ullah,

Huyop,

Wahab

et al. 2024

Heliyon

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“…Pada tumbuhan, isolasi DNA dapat dilakukan pada berbagai jaringan, misalnya daun dan ranting, termasuk pada jaringan kayu yang keras sekalipun, seperti pada kayu sonokeling. Pada saat ini sudah banyak dikembangkan berbagai teknik isolasi DNA seperti menggunakan teknik dasar CTAB (Murray & Thompson, 1980;Doyle & Doyle, 1987;Rogers & Bendich, 1989;Doyle, 1991;Lodhi et al, 1994) dan juga perangkat/kit komersial untuk isolasi DNA misalnya Genomic DNA Mini Kit Plant (Geneaid) dan Dneasy Plant Mini Kit (Qiagen) (Yulita et al, 2020(Yulita et al, , 2022a(Yulita et al, , 2022b(Yulita et al, , 2022cHong et al, 2022;Marinček et al, 2022;Şapcı-Selamoğlu, 2022;Cunill-Flores et al, 2023;Jihong et al, 2023;Guillardín & MacKay, 2023). Penelitian ini bertujuan untuk membandingkan teknik isolasi DNA yang berasal dari jaringan kayu dan daun sonokeling dengan menggunakan teknik dasar CTAB dan kit komersial, dan untuk mendukung kegiatan pengembangan pangkalan data DNA sonokeling.…”

Section: Pendahuluanunclassified

“…Amplicon PCR dengan markah intron trnG (Gambar 6a) dan petD−rpoA (Gambar 6b) pada sampel DNA kayu sonokeling menggunakan protokol Genomic DNA Mini Kit Plant jauh lebih baik dibandingkan dengan protokol CTAB. Hal ini sejalan dengan hasil laporan Yulita et al (2022a) yang menyatakan bahwa protokol terbaik untuk mengisolasi DNA kayu sonokeling adalah dengan menggunakan kit komersial. Untuk meningkatkan hasil dari protokol tersebut, perlu dilakukan lebih banyak pengujian optimalisasi kondisi PCR dan pemurnian DNA hasil isolasi (Yulita et al, 2022a)…”

Section: Dna Genomik Hasil Isolasiunclassified

“…Hal ini sejalan dengan hasil laporan Yulita et al (2022a) yang menyatakan bahwa protokol terbaik untuk mengisolasi DNA kayu sonokeling adalah dengan menggunakan kit komersial. Untuk meningkatkan hasil dari protokol tersebut, perlu dilakukan lebih banyak pengujian optimalisasi kondisi PCR dan pemurnian DNA hasil isolasi (Yulita et al, 2022a)…”

Section: Dna Genomik Hasil Isolasiunclassified

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Perbandingan Teknik Isolasi DNA pada Daun dan Kayu Sonokeling (Dalbergia latifolia)

Susila,

I Putu Gede P. Damayanto,

Kusumadewi Sri Yulita

et al. 2024

MANILKARA: j. Journal of Bioscience

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Sonokeling (Dalbergia latifolia) is a hardwood plant, resistant to termite attack, its wood has beautiful patterned fibers and textures, so it is widely used for furniture in luxury categories with high economic value. According to CITES (Convention on International Trade in Endagered Species of Wild Fauna and Flora), rosewood trade status is included in appendix II. In the trade of rosewood in both domestic and international markets, there are relatively many obstacles and difficulties in identifying the origin of rosewood. It is very important to establish a reference on the identity of wood based on the location of origin as an effort to prevent illegal trade, namely by using a molecular-based identification approach (DNA fingerprinting). In the isolation of rosewood DNA, special techniques are required due to its hard wood characteristics. This study aims to compare DNA isolation techniques from rosewood and rosewood leaves using the CTAB (Cetyl Trimethyl Ammonium Bromide) protocol and the DNA Mini Kit Plant protocol. The research used molecular biology-based identification methods. PCR results showed that the DNA isolation protocol using the commercial kit outperformed the CTAB protocol for both leaf and wood samples. DNA isolation from wood samples was more difficult than leaf samples. Overall, the best protocol for DNA isolation from rosewood leaves and wood was to use a commercial kit.

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Omics Approaches in Conservation and Management of Forest Genetic Resources

Sarath,

Bhasker,

Balakrishnan

et al. 2024

Biotechnological Approaches for Sustaining Forest Trees and Their Products

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Population genetic of the Indonesian rosewood (Dalbergia latifolia) from Java and West Nusa Tenggara revealed using sequence-related amplified polymorphism

Cited by 4 publications

References 46 publications

Using pollen DNA metabarcoding to trace the geographical and botanical origin of honey from Karangasem, Indonesia

Using pollen DNA metabarcoding to trace the geographical and botanical origin of honey from Karangasem, Indonesia

Perbandingan Teknik Isolasi DNA pada Daun dan Kayu Sonokeling (Dalbergia latifolia)

Omics Approaches in Conservation and Management of Forest Genetic Resources

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