Population estimate and morphometry of ovarian preantral follicles from three recently recognized squirrel monkey species: a comparative study

Lopes, Gerson Paulino; Santos, Regiane R.; Almeida, D V C; Brito, A. B.; Queiroz, Helder Lima de; Domingues, Sheyla Farhayldes Souza

doi:10.1017/s0967199417000107

Cited by 3 publications

(2 citation statements)

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“…Interesting, both species presented secondary follicles and oocytes with similar dimensions. Dimensions of primordial, transition and primary follicles were also similar to those of Saimiri cassiquiarensis, S. macrodon and S. vanzolinii (Lopes et al, 2017), except for the oocyte nucleus, which was larger in S. apella. Moreover, preantral follicles from P. anubis (Amorim et al, 2016) were twice as big as those from S. apella.…”

Section: Discussionmentioning

confidence: 58%

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Morphology and morphometry of preantral follicles, and immunolocalization of angiogenic factors in ovarian tissue from the neotropical primateSapajus apella

Brito

Silva³

et al. 2018

Zygote

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SummaryOvarian biopsies from five health adult monkeys were collected by exploratory laparotomy. Preantral follicles (primordial, primary, and secondary) were classified as normal or degenerated and submitted to morphometric analysis in which granulosa cell counts and the areas of follicles, oocytes, and oocyte nuclei were measured. Ovarian fragments were also immunolabelled for the quantitative analysis of VEGFA and CD31 protein expression in the ovarian tissue and in the preantral follicles. In total, 213 preantral follicles was examined for morphometry and morphological classification. From this total, 20 (9.4%) were follicles enclosing two or more oocytes, i.e. multi-oocyte follicles (MOFs). From the 193 follicles enclosing only one oocyte, 46.3% were classified as primordial, 24,1% as transition, 23.3% as primary, and 6.3% as secondary follicles. The mean number of granulosa cells surrounding primordial, transition, primary, and secondary follicles was 9.2, 12.1, 18.7, and 45.3, respectively. Increase in oocyte diameter was observed from primary to secondary follicles, while the oocyte nucleus increased only when follicles reached the secondary stage. The expression of CD31 was strong in vessels, corpus luteum, and in normal oocytes and granulosa cells from preantral follicles at all developmental stages. Likewise, VEGFA expression was observed in vessels and preantral follicles (granulosa cells, the oocyte and the oocyte nucleus). We characterized the morphology, and morphometry and expression of angiogenic factors in normal and atretic preantral follicles fromSapajus apella. This description can support the analysis of follicular quality and survival after procedures such as transplantation and cryopreservation.

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Section: Discussionmentioning

confidence: 58%

“…macrodon and S . vanzolinii (Lopes et al , 2017), except for the oocyte nucleus, which was larger in S . apella .…”

Section: Discussionmentioning

confidence: 97%