Population Dynamics of Thermotolerant<i>Campylobacter</i>in Broilers Reared on Reused Litter

Würfel, Simone de Fátima Rauber; Voss-Rech, Daiane; Pozza, J. dos S.; Coldebella, Arlei; Silva, Virgínia Santiago; Vaz, C. S. L.

doi:10.1089/fpd.2019.2645

Cited by 11 publications

(8 citation statements)

References 26 publications

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“…Thus, the question of the environmental transmission routes arises, particularly concerning animal reservoirs such as poultry and ruminants that could contribute to water contamination (Mughini-Gras et al, 2016). Persistent strains have already been identified, mainly in poultry farms and in milk, and it would be interesting to link lineage A with other contamination sources such as insects, rodents, drinking water, or the surrounding environment (Kudirkienėet al, 2010;Perez-Boto et al, 2012;Rauber-Würfel et al, 2019;Jaakkonen et al, 2020). The lineage B (ST2254-gyrA9-porA1) arose unexpectedly from our national surveillance with an epidemic curve between March and April 2014 (>70 campylobacteriosis cases).…”

Section: Discussionmentioning

confidence: 99%

Investigating Major Recurring Campylobacter jejuni Lineages in Luxembourg Using Four Core or Whole Genome Sequencing Typing Schemes

Nennig

Llarena

Herold

et al. 2021

Front. Cell. Infect. Microbiol.

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Campylobacter jejuni is the leading cause of bacterial gastroenteritis, which has motivated the monitoring of genetic profiles circulating in Luxembourg since 13 years. From our integrated surveillance using a genotyping strategy based on an extended MLST scheme including gyrA and porA markers, an unexpected endemic pattern was discovered in the temporal distribution of genotypes. We aimed to test the hypothesis of stable lineages occurrence by implementing whole genome sequencing (WGS) associated with comprehensive and internationally validated schemes. This pilot study assessed four WGS-based typing schemes to classify a panel of 108 strains previously identified as recurrent or sporadic profiles using this in-house typing system. The strain collection included four common lineages in human infection (N = 67) initially identified from recurrent combination of ST-gyrA-porA alleles also detected in non-human samples: veterinary (N = 19), food (N = 20), and environmental (N = 2) sources. An additional set of 19 strains belonging to sporadic profiles completed the tested panel. All the strains were processed by WGS by using Illumina technologies and by applying stringent criteria for filtering sequencing data; we ensure robustness in our genomic comparison. Four typing schemes were applied to classify the strains: (i) the cgMLST SeqSphere+ scheme of 637 loci, (ii) the cgMLST Oxford scheme of 1,343 loci, (iii) the cgMLST INNUENDO scheme of 678 loci, and (iv) the wgMLST INNUENDO scheme of 2,795 loci. A high concordance between the typing schemes was determined by comparing the calculated adjusted Wallace coefficients. After quality control and analyses with these four typing schemes, 60 strains were confirmed as members of the four recurrent lineages regardless of the method used (N = 32, 12, 7, and 9, respectively). Our results indicate that, regardless of the typing scheme used, epidemic or endemic signals were detected as reflected by lineage B (ST2254-gyrA9-porA1) in 2014 or lineage A (ST19-gyrA8-porA7), respectively. These findings support the clonal expansion of stable genomes in Campylobacter population exhibiting a multi-host profile and accounting for the majority of clinical strains isolated over a decade. Such recurring genotypes suggest persistence in reservoirs, sources or environment, emphasizing the need to investigate their survival strategy in greater depth.

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Section: Discussionmentioning

confidence: 99%

Investigating Major Recurring Campylobacter jejuni Lineages in Luxembourg Using Four Core or Whole Genome Sequencing Typing Schemes

Nennig

Llarena

Herold

et al. 2021

Front. Cell. Infect. Microbiol.

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“…A study conducted by our group (Rossler et al., 2020) suggested that Campylobacter may be present in farms before flock placement, possibly at levels undetectable by conventional sampling and culture techniques. Litter, flies and other insects, wild‐life birds and farm workers' boots can be Campylobacter reservoirs and a source of infection among flocks (Rauber Würfel et al, 2019; Schets et al., 2017). If Campylobacter can persist at farm in different reservoirs, it could favour horizontal transmission and its dissemination in consecutive rearings (Smith, Messam, et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Impact of farm‐level strategies against thermotolerant Campylobacter in broiler chickens, using a quantitative risk assessment model and meta‐analysis

Zbrun

Olivero

Soto

et al. 2022

Zoonoses and Public Health

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The aim of this study was to evaluate the effect of control strategies (probiotic supplementation and vaccination) at farm on thermotolerant Campylobacter infection due to the consumption of salad cross‐contaminated from broiler meat. The broiler food chain was modelled considering the most common practices applied in Argentina (baseline model) and their effect on the prevalence and counts of Campylobacter. Probiotic supplementation and vaccination against Campylobacter spp. were included in different models to evaluate their effectiveness. The parameter distributions of each intervention were obtained based on a systematic review and meta‐analysis previously described. The control measures applied at farms were evaluated considering their effectiveness in reducing both the prevalence and the count of thermotolerant Campylobacter in comparison with the baseline model estimation and expressed as relative change in campylobacteriosis risk. Additionally, the identification of the most important input parameters for the model was performed by sensitivity analysis. The model estimated a risk of campylobacteriosis per consumed serving of salad contaminated with poultry meat of 4.99 x 10–3 (95% CI: 6.12 x 10–6–1.13 x 10–2), corresponding to an annual incidence risk estimated of 1,876,009 persons. Scenario analysis indicated that the application of vaccines against Campylobacter (probability of campylobacteriosis = 9.55 x 10–4; 95% CI: 5.31 x 10–4–1.29 x 10–3) and the supplementation of broilers with probiotics (probability of campylobacteriosis = 1.32 x 10–3; 8.55 x 10–4–1.69 x 10–3) can offer a modest reduction in risk estimates. The intervention efficacy was 80.86% and 73.54% for vaccination and probiotic supplementation, respectively. On‐farm interventions were effective to mitigate the risk of campylobacteriosis.

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“…Compared to Salmonella , the grow-out period was not included in the final regression model for Campylobacter ( Table 4 ), however, Campylobacter prevalence has been reported to be higher during late grow-out than early grow-out ( Stern et al, 2001 ; Chinivasagam et al, 2016 ; Rauber et al, 2019 ). Chinivasagam et al (2016) found only 1 Campylobacter positive litter sample on d 7 and 9 of grow-out while 33.3 to 86% of the litter samples were positive later in the grow-out cycles (d 27–51).…”

Section: Discussionmentioning

confidence: 99%

“…Understanding broiler management factors that could be used to predict the occurrence of food-borne pathogens in reused litter is vital to limiting their prevalence in post-harvest chicken production. Although there are studies on the microbiology of reused litter, these studies have been limited to wood shavings and rice-hull based litter ( Kelley et al, 1995 ; Brooks et al, 2010 ; Roll et al, 2011 ; Volkova et al, 2011 ; Chinivasagam et al, 2012 ; Eberle et al, 2013 ; Roberts et al, 2013 ; Wei et al, 2013 ; Brooks et al, 2016 ; Rauber Wurfel et al, 2019 ; Voss-Rech et al, 2019 ; Valeris-Chacin et al, 2021 ; Chinivasagam et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Management and environmental factors influence the prevalence and abundance of food-borne pathogens and commensal bacteria in peanut hull-based broiler litter

Oladeinde

Awosile²,

Woyda³

et al. 2023

Poultry Science

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Population Dynamics of ThermotolerantCampylobacterin Broilers Reared on Reused Litter

Cited by 11 publications

References 26 publications

Investigating Major Recurring Campylobacter jejuni Lineages in Luxembourg Using Four Core or Whole Genome Sequencing Typing Schemes

Investigating Major Recurring Campylobacter jejuni Lineages in Luxembourg Using Four Core or Whole Genome Sequencing Typing Schemes

Impact of farm‐level strategies against thermotolerant Campylobacter in broiler chickens, using a quantitative risk assessment model and meta‐analysis

Management and environmental factors influence the prevalence and abundance of food-borne pathogens and commensal bacteria in peanut hull-based broiler litter

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