Population dynamics of rumen microbes using modern techniques in rumen enhanced solid incubation

Raizada, Neena; Sonakya, V.; Dalhoff, R.; Hausner, Martina; Wilderer, P.A.

doi:10.2166/wst.2003.0234

Cited by 5 publications

(3 citation statements)

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“…Acetotrophic methanogens were inhibited strongly below a pH of 6.2, while free ammonia (FA) concentrations could also be inhibitory at pH levels above 7.4. During two-stage anaerobic digestion of grass, it was observed that the stability of the methanogenic reactor, which was inoculated with fresh rumen resulted due to the dominance of Methanosaeta species (mainly the filamentous type) (Raizada et al 2003).…”

Section: The Influence Of Operational and Environmental Parametersmentioning

confidence: 99%

The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review

Demirel

Scherer

2008

Rev Environ Sci Biotechnol

966

497

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Among different conversion processes for biomass, biological anaerobic digestion is one of the most economic ways to produce biogas from various biomass substrates. In addition to hydrolysis of polymeric substances, the activity and performance of the methanogenic bacteria is of paramount importance during methanogenesis. The aim of this paper is primarily to review the recent literature about the occurrence of both acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of particulate biomass to methane (not wastewater treatment), while this review does not cover the activity of the acetate oxidizing bacteria. Both acetotrophic and hydrogenotrophic methanogens are essential for the last step of methanogenesis, but the reports about their roles during this phase of the process are very limited. Despite, some conclusions can still be drawn. At low concentrations of acetate, normally filamentous Methanosaeta species dominate, e.g., often observed in sewage sludge. Apparently, high concentrations of toxic ionic agents, like ammonia, hydrogen sulfide (H 2 S) and volatile fatty acids (VFA), inhibit preferably Methanosaetaceae and especially allow the growth of Methanosarcina species consisting of irregular cell clumps, e.g., in cattle manure. Thermophilic conditions can favour rod like or coccoid hydrogenotrophic methanogens. Thermophilic Methanosarcina species were also observed, but not thermophilic Methanosaetae. Other environmental factors could favour hydrogentrophic bacteria, e.g., short or low retention times in a biomass reactor. However, no general rules regarding process parameters could be derivated at the moment, which favours hydrogenotrophic methanogens. Presumably, it depends only on the hydrogen concentration, which is generally not mentioned in the literature.

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Section: The Influence Of Operational and Environmental Parametersmentioning

confidence: 99%

The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review

Demirel

Scherer

2008

Rev Environ Sci Biotechnol

966

497

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“…The broad range of metabolic capabilities of different microorganisms in the rumen ecosystem is indicative of numerous inter-species interactions at metabolic, signaling, and regulatory levels. While the major functions of Bacteroides, Firmicutes and Archaea, and the interplay between them is commonly known (Hungate, 1975; Wolin, 1979; Flint, 1997; Raizada et al, 2003; Henderson et al, 2015; Nagaraja, 2016; Anderson et al, 2017; Liu et al, 2017), the current knowledge is only partial. As described in the methods section, a detailed step-by-step procedure was employed to identify 22 potential metabolic transactions.…”

Section: Resultsmentioning

confidence: 99%

Metabolic Modeling Elucidates the Transactions in the Rumen Microbiome and the Shifts Upon Virome Interactions

2019

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The complex microbial ecosystem within the bovine rumen plays a crucial role in host nutrition, health, and environmental impact. However, little is known about the interactions between the functional entities within the system, which dictates the community structure and functional dynamics and host physiology. With the advancements in high-throughput sequencing and mathematical modeling, in silico genome-scale metabolic analysis promises to expand our understanding of the metabolic interplay in the community. In an attempt to understand the interactions between microbial species and the phages inside rumen, a genome-scale metabolic modeling approach was utilized by using key members in the rumen microbiome (a bacteroidete, a firmicute, and an archaeon) and the viral phages associated with them. Individual microbial host models were integrated into a community model using multi-level mathematical frameworks. An elaborate and heuristics-based computational procedure was employed to predict previously unknown interactions involving the transfer of fatty acids, vitamins, coenzymes, amino acids, and sugars among the community members. While some of these interactions could be inferred by the available multi-omic datasets, our proposed method provides a systemic understanding of why the interactions occur and how these affect the dynamics in a complex microbial ecosystem. To elucidate the functional role of the virome on the microbiome, local alignment search was used to identify the metabolic functions of the viruses associated with the hosts. The incorporation of these functions demonstrated the role of viral auxiliary metabolic genes in relaxing the metabolic bottlenecks in the microbial hosts and complementing the inter-species interactions. Finally, a comparative statistical analysis of different biologically significant community fitness criteria identified the variation in flux space and robustness of metabolic capacities of the community members. Our elucidation of metabolite exchange among the three members of the rumen microbiome shows how their genomic differences and interactions with the viral strains shape up a highly sophisticated metabolic interplay and explains how such interactions across kingdoms can cause metabolic and compositional shifts in the community and affect the health, nutrition, and pathophysiology of the ruminant animal.

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“…Numerous bioreactors were designed to degrade the lignocellulosic biomass with rumen microbial consortium. The processes developed in this field range from the very simple apparatus such as a batch reactor (Cerrato-Sanchez et al 2007;Hu et al 2004Hu et al , 2007Raizada et al 2003;Seon et al 2003) to a complicated apparatus designed to work on the continuous mode and even equipped with semipermeable membranes to remove the VFAs produced (Czerkawski and Cheng 1988;Dalhoff et al 2003;Gijzen et al 1988;Nair et al 2005). These anaerobic digestion processes were related to both animal physiology and environmental bioengineering in order to respectively improve the nutrition of livestock as in the rumen simulation technique (RUSI-TEC) and to improve the production of biogas from lignocellulosic wastes as in the rumen derived anaerobic digestion system (RUDAD).…”

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confidence: 99%

Animal digestive strategies versus anaerobic digestion bioprocesses for biogas production from lignocellulosic biomass

Bayané

Guiot

2010

Rev Environ Sci Biotechnol

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Herbivorous mammals and wood-eating insects are fairly effective in the digestion of plant polymers, such as lignocellulosics. In order to improve methane production from the lignocellulosic biomass, several kinds of anaerobic digestion processes derived from animal models have been devised. However, the rates of biodegradation occurring in the anaerobic bioreactors still remain lower than in animal guts. The effectiveness of the digestive systems of those animals results from the concerted action of the various enzymes (e.g. cellulases, xylanases, esterases, ligninases) produced in their guts as well as their integration with mechanical and chemical actions. Powerful pretreatment (prefermentation) operations are integrated to and support efficiently the microbial fermentation system, e.g. the rumination (i.e. mechanical) in ruminants and the secretion of endogenous cellulases (i.e. enzymatic) or the alkaline treatment (chemical) at mid-way in xylophagous insects. The oxygen gradients along the gastrointestinal tract may also stimulate the hydrolytic activities of some microbial populations. In addition, the solid retention time, the digesta flow and the removal of the end-products are well ordered to enable animals to thrive on a complex polymer such as lignocellulose. At the same time, technologies were developed to degrade lignocellulosic biomass, such as the rumen derived anaerobic digestion (RUDAD) process and the rumen simulating technique (RUSITEC), more elaborated and using rumen microbial consortia. Overall, they showed that the fermentation taking place in the rumen fermentation and even in the hindgut are biological processes that go beyond the limited environmental conditions generally found in anaerobic digesters. Hence, knowledge on herbivores' digestion mechanisms might be better exploited in the design and operation of anaerobic digesters. This literature review is a cross-analysis of the relevant information about the digestive strategies of herbivorous and wood-eating animals and the bioengineering techniques in lignocelluloses degradation. The aim is to highlight strategies of animals' digestion simulation for more effective anaerobic digestion of lignocellulosic compounds and other solid residues.The main structural polysaccharides of plant tissues, cellulose and hemicelluloses, are linked with lignin and together called lignocellulose. Lignocellulosic biomass is more and more considered as a potential

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Population dynamics of rumen microbes using modern techniques in rumen enhanced solid incubation

Cited by 5 publications

References 0 publications

The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review

The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review

Metabolic Modeling Elucidates the Transactions in the Rumen Microbiome and the Shifts Upon Virome Interactions

Animal digestive strategies versus anaerobic digestion bioprocesses for biogas production from lignocellulosic biomass

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