Population dynamics of mites of the family pyroglyphidae and micromycetes in laboratory cultures

Петрова-Никитина, А.Д.; Antropova, A. B.; Биланенко, Е. Н.; Мокеева, В. Л.; Chekunova, L. N.; Bulgakova, Tatyana A.; Zheltikova, T. M.

doi:10.1134/s0013873811030134

Cited by 7 publications

(2 citation statements)

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“…We hypothesize that mite feeding on the yeasts leads to overgrazing and replacement by A. penicillioides. A previous study showed that this fungus was introduced to the culture via mites [37]. The microbiome analyses in the current study supports this nding, because the mites introduced into the culture contained 50% of A. penicillioides reads.…”

Section: Discussionsupporting

confidence: 88%

“…The microbiome analyses in the current study supports this nding, because the mites introduced into the culture contained 50% of A. penicillioides reads. The density of the fungus (observed as the numbers of its spores) increased in the culture with increasing time and mite density [37]. Here we detected a positive relationship between guanine and the relative abundance of amplicons derived from A. penicillioides in the environmental culture, and this may indicate that this fungus uses guanine as a source of nitrogen for mycelial growth.…”

Section: Discussionsupporting

confidence: 51%

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Microbiome variation during culture growth of the European house dust mite,Dermatophagoides pteronyssinus

Nešvorná

Pekár

Shcherbachenko

et al. 2021

FEMS Microbiology Ecology

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In culture, the house dust mite, Dermatophagoides pteronyssinus, shows different growth patterns, but the composition and changes in the associated microbial community during mite culture growth are poorly known. In this study, we analyzed temporal changes in microbial communities including ‘internal’ communities (inside mites, ingested) and ‘environmental’ communities (from culture environment). Microbial community structure was correlated with guanine content (a nitrogenous waste product of mites) and mite population density. Both internal and environmental microbial communities were remarkably consistent between biological replicates from the same culture age group and were composed of relatively few dominant taxa–11 bacterial and 3 fungal operational taxonomic units (OTUs). Significant changes over time in microbial community structure in the bulk culture environment and in internal mite samples were observed. The yeast, Saccharomyces cerevisiae, a main component of the mite diet, gradually disappeared during mite culture growth and was replaced by fungi from the genera Aspergillus and Candida in both ‘internal’ and ‘environmental’ samples. In environmental samples, bacteria from the genus Lactobacillus and S. cerevisiae were negatively correlated, and Aspergillus and Candida positively correlated, with guanine content. The relative abundance of bacteria from the genus Kocuria increased with mite density but declined with increasing guanine content. The relative abundance of bacteria from the genus Virgibacillus was negatively correlated with mite density in ‘internal’ samples. Gram-positive bacteria dominated bacterial microbiomes at all time points in our experiments, indicating a more limited possibility for vaccine contamination by bacterial endotoxins (heat-stable lipopolysaccharides produced mostly by Gram-negative bacteria) in our experimental cultures.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 51%