Population dynamics of<i>Epicoccum nigrum</i>, a biocontrol agent against brown rot in stone fruit

Cal, A. De; Larena, Inmaculada; Liñán, María García; Torres, R.; Lamarca, N.; Usall, J.; Domenichini, P.; Bellini, A.; Eribe, X. Ochoa de; Melgarejo, P.

doi:10.1111/j.1365-2672.2008.04030.x

Cited by 56 publications

(30 citation statements)

References 40 publications

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“…We cannot comment at this stage on the morphospecies, but three of the fungal genera identified, Epicoccum, Trichoderma and Ulocladium, contain mycoparasitic species. Some endophytic Epicoccum species have been screened as potential biocontrol agents of fungal pathogens of diverse crops, including potato, sugarcane and stone fruits (De Cal et al 2009;Lahlali and Hijri 2010;Fávaro et al 2012); whilst Trichoderma is a well known mycoparasitic genus that has been widely used for biocontrol of plant pathogens (Harman 2000; Holmes et al 2004)). Similarly, some Ulocladium species have also generated interest as mycoparasites of plant pathogens, and Kohl et al (1997) reported that Ulocladium atrum is antagonistic to Botrytis disease in onions.…”

Section: Fig 2 Continuedmentioning

confidence: 99%

Quality control by leaf-cutting ants: evidence from communities of endophytic fungi in foraged and rejected vegetation

Rocha

Jorge

Lúcia

et al. 2014

Arthropod-Plant Interactions

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Leaf-cutting ants of the genera Acromyrmex and Atta forage vegetation for incorporation into their mutualistic fungal gardens. However, the presence of certain endophytic fungi in this predominantly leaf-based material could affect the fungal garden and thus the choice of material by the ants. The present study was conducted to document the endophytic fungal communities occurring in the vegetation being transported by workers of Atta laevigata into their nests and to compare this community structure with that of the vegetative material subsequently rejected from the nests. We found considerable diversity in the fungi isolated. Acremonium, Cylindrocladium, Drechslera, Epicoccum, Fusarium, Trichoderma, Ulocladium and two unidentified morphospecies were significantly more common in rejected compared with foraged material, and some of these genera include mycoparasites, which could represent a threat to the fungal gardens. Conversely, Colletotrichum, Pestalotiopsis, Phomopsis, Xylaria and an unidentified morphospecies were more common in carried compared with rejected material. The possibility that ants have a 'quality-control' mechanism based on the presence of antagonistic fungal endophytes is discussed, as is the potential use of these fungi as biocontrol agents against Attini pests.

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Section: Fig 2 Continuedmentioning

confidence: 99%

Quality control by leaf-cutting ants: evidence from communities of endophytic fungi in foraged and rejected vegetation

Rocha

Jorge

Lúcia

et al. 2014

Arthropod-Plant Interactions

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“…in peaches and nectarines [7], [8], [9], against Sclerotinia sclerotiorum in sunflowers [10] and against Pythium in cotton [11]. Many studies have focused on the ability of this fungus to produce antimicrobial compounds such as epicorazins A–B [12], epicoccins A–D [13], epicoccarines A–B and epipyridone [14], flavipin [15] and epirodins [16].…”

Section: Introductionmentioning

confidence: 99%

Polyphasic Analysis of Intraspecific Diversity in Epicoccum nigrum Warrants Reclassification into Separate Species

et al. 2011

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Background Epicoccum nigrum Link (syn. E. purpurascens Ehrenb. ex Schlecht) is a saprophytic ascomycete distributed worldwide which colonizes a myriad of substrates. This fungus has been known as a biological control agent for plant pathogens and produces a variety of secondary metabolites with important biological activities as well as biotechnological application. E. nigrum produces darkly pigmented muriform conidia on short conidiophores on sporodochia and is a genotypically and phenotypically highly variable species. Since different isolates identified as E. nigrum have been evaluated as biological control agents and used for biocompound production, it is highly desirable that this species name refers to only one lineage. However, according to morphological and genetic variation, E. nigrum present two genotypes that may comprise more than one species.Methodology/Principal FindingsWe report the application of combined molecular (ITS and β-tubulin gene sequence analysis, PCR-RFLP and AFLP techniques), morphometric, physiological, genetic compatibility and recombination analysis to study the taxonomic relationships within an endophytic population that has been identified as E. nigrum. This combined analysis established two genotypes showing morphological, physiological and genetic divergence as well as genetic incompatibility characterized by colony inhibition, strongly indicating that these genotypes correspond to different species. Genotype 1 corresponds to E. nigrum while genotype 2 represents a new species, referred to in this study as Epicoccum sp.Conclusions/SignificanceThis research contributes to the knowledge of the Epicoccum genus and asserts that the classification of E. nigrum as a single variable species should be reassessed. In fact, based on the polyphasic approach we suggest the occurrence of cryptic species within E. nigrum and also that many of the sequences deposited as E. nigrum in GenBank and culture collection of microbial strains should be reclassified, including the reference strain CBS 161.73 sequenced in this work. In addition, this study provides valuable tools for differentiation of Epicoccum species.

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“…E. nigrum has been used as a biological control agent on peaches and nectarines orchards against Monilinia spp. [28] and Pythium cotton [29].…”

Section: Discussionmentioning

confidence: 99%

Composition of endophytic fungal community associated with leaves of maize cultivated in south Brazilian field

Szilagyi-Zecchin¹,

Adamóski²,

Gomes³

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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The objective of this study was to conduct a survey about fungi associated with leaves from two different maize plant lineages and to analyze their microbiota diversity. Isolated fungi were identified by morphological analysis and molecular taxonomy was performed using ITS1-5.8S-ITS2 rDNA. About 27 fungi morphotypes were obtained, 15 of them were from the first maize lineage. About 86.7% of the individuals belonged to the Dothideomycetes class (Phoma sorghina, Epicocum nigrum, Cladosporium sp., Bipolaris zeicola, and Alternaria alternata complex) and 13.3% to the Sordariomycetes class (Diaporthe/Phomopsis sp. and Nigrospora sp.). This ratio was opposite in the other maize lineage with 25.0% of Dothideomycetes (E. nigrum and Pleosporales) and 75.0% of Sordariomycetes (Gibberella fujikuroi complex, Fusarium graminearum complex, Diaporthe/Phomopsis sp., and Nigrospora sp.). By concerning the analyses of morphological characteristics and molecular phylogeny, this study intended to identify the groups of saprophytic, phytopathogenic, and mycotoxin fungi, which differently co-inhabit leaf tissue of maize plants in both tested lineages.

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Population dynamics ofEpicoccum nigrum, a biocontrol agent against brown rot in stone fruit

Cited by 56 publications

References 40 publications

Quality control by leaf-cutting ants: evidence from communities of endophytic fungi in foraged and rejected vegetation

Quality control by leaf-cutting ants: evidence from communities of endophytic fungi in foraged and rejected vegetation

Polyphasic Analysis of Intraspecific Diversity in Epicoccum nigrum Warrants Reclassification into Separate Species

Composition of endophytic fungal community associated with leaves of maize cultivated in south Brazilian field

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