Population dynamics of filamentous bacteria in Danish wastewater treatment plants with nutrient removal

Mielczarek, Artur Tomasz; Kragelund, Caroline; Eriksen, Poul Svante; Nielsen, Per Halkjær

doi:10.1016/j.watres.2012.04.009

Cited by 110 publications

(74 citation statements)

References 62 publications

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“…Despite the microbiological methods used, plenty of the studies on microbial communities of activated sludge bioreactors have been conducted to profile taxonomic information based on 16S rRNA genes, using technical approaches of clone library (Wells et al 2011;Yang et al 2011), denaturing gradient gel electrophoresis (DGGE) (Yang et al 2011), terminal restriction fragment length polymorphism (T-RELP) (Wells et al 2011;Yang et al 2011), fluorescence in situ hybridization (FISH) (Mielczarek et al 2012(Mielczarek et al , 2013, Illumina (Albertsen et al 2012), 454-pyrosequencing (Hu et al 2012;Wang et al 2012a), and PhyloChips (Xia et al 2010). However, 16S rRNA gene-based ways may not be efficient in distinguishing some closely related but ecologically distinct groups of microorganisms as a consequence of the low evolutionary rate of 16S rRNA genes (Palys et al 1997).…”

Section: Introductionmentioning

confidence: 99%

“…Earlier studies using the methods of T-RFLP and clone library indicated that the detected richness (numbers of operational taxonomic units (OTUs)) and dominant phyla and OTUs did not change much (Wells et al 2011) and revealed relatively stable AOB structures in full-scale wastewater treatment systems with relatively stable treatment efficiencies during a year of monitoring. Other studies applying FISH suggested that the relative abundances of filamentous bacteria (Mielczarek et al 2012), and abundant polyphosphate-accumulating organisms (PAOs) (Mielczarek et al 2013) showed minor changes in full-scale WWTPs with good treatment efficiencies during 3-year investigations. From this perspective, we considered that a single grab of sample from functionally stable systems could be effective, as it could at least reveal microbial richness and abundant groups.…”

Section: Introductionmentioning

confidence: 99%

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Overall functional gene diversity of microbial communities in three full-scale activated sludge bioreactors

Xia

Wang

Wen

et al. 2014

Appl Microbiol Biotechnol

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Understanding microbial community composition is thought to be crucial for improving process functioning and stabilities of full-scale activated sludge reactors in wastewater treatment plants (WWTPs). However, functional gene compositions of microbial communities within them have not been clearly elucidated. To gain a complete picture of microbial community, in this study, GeoChip 4.2 was used to profile the overall functional genes of three full-scale activated sludge bioreactors, the 16S rRNA gene diversities of which had been unveiled by 454-pyrosequencing in our previous investigation. Triplicate activated sludge samples from each system were analyzed, with the detection of 38,507 to 40,654 functional genes. A high similarity of 77.3-81.2 % shared functional genes was noted among the nine samples, verified by the high 16S rRNA gene similarity with shared operational taxonomic units (OTUs) constituting 66.4-70.0 % of the detected sequences in each system. Correlation analyses showed that the abundances of a wide array of functional genes were associated with system performances. For example, the abundances of carbon degradation genes were strongly correlated to chemical oxygen demand (COD) removal efficiencies (r=0.8697, P<0.01). Lastly, we found that sludge retention time (SRT), influent total nitrogen concentrations (TN inf), and dissolved oxygen (DO) concentrations were key environmental factors shaping the overall functional genes. Together, the results revealed vast functional gene diversity and some links between the functional gene compositions and microbe-mediated processes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overall functional gene diversity of microbial communities in three full-scale activated sludge bioreactors

Xia

Wang

Wen

et al. 2014

Appl Microbiol Biotechnol

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show abstract

“…Badania przeprowadzone z pomocą nowoczesnych technik biologii molekularnej w 28 oczyszczalniach ścieków w Danii wykazały, że bakterie nitkowate stanowią przeciętnie około 24% bakterii tworzących kłaczki osadu czynnego [11]. Wiele wskazuje na to, że bakterie nitkowate odgrywają kluczową rolę w formowaniu kłaczków osadu czynnego tworząc szkielet, do którego przyłączają się bakterie zooglealne [2].…”

Section: Wstępunclassified

New automated procedure to determine the contribution of filamentous bacteria in activated sludge flocs

Liwarska-Bizukojć¹,

Bizukojć²

2013

JCEEA

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“…The relatively high observed abundances of these groups indicates that they are likely the most important filamentous organisms in these systems (Mielczarek et al, 2012;McIlroy et al, 2015b) and they simply must make a substantial contribution to the bulk nutrient transformations observed. Although the ecophysiology of the 'Candidatus Microthrix parvicella' and P2CN44 phylotypes has been described (Kragelund et al, 2011;McIlroy et al, 2013), minimal ecological and physiological information is available for members of the B45 phylotype.…”

Section: Introductionmentioning

confidence: 99%

“…Semiquantitative survey data suggest that these organisms are particularly well suited to flourish under the dynamic electron acceptor conditions, where carbon substrates prevail under the anaerobic conditions, characteristic of plants designed to remove phosphorus (P) and nitrogen (N) (Speirs et al, 2009;Mielczarek et al, 2012). This suggests an ability for anaerobic carbon storage and/or respiration/fermentation.…”

Section: Introductionmentioning

confidence: 99%

Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge

et al. 2016

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Overgrowth of filamentous bacteria in activated sludge wastewater treatment plants (WWTPs) leads to impaired sludge settleability, a condition known as bulking, which is a common operational problem worldwide. Filaments with the Eikelboom 0092 morphotype are commonly associated with such bulking episodes. Members of the uncultured B45 phylotype, which is embraced within the phylum Chloroflexi, were recently shown to exhibit this morphology. Although these organisms are among the most abundant populations recorded in activated sludge processes, nothing is known about their metabolic characteristics. In this study, a genome sequence, representing the B45 phylotype, was retrieved from a metagenome generated from an activated sludge WWTP. The genome consisted of two chromosomes and one plasmid, which were 4.0, 1.0 and 0.04 Mbps in size, respectively. A metabolic model was constructed for this organism, based on annotation of its genome, showing its ability to generate energy by respiration, utilizing oxygen, nitrite or nitrous oxide as electron acceptors, or by fermentation of sugars. The ability of B45 members to ferment sugars under anaerobic conditions was validated in situ with microautoradiography-fluorescence in situ hybridization. The provisional name of 'Candidatus Promineofilum breve' is proposed for this species. This study represents the first detailed information on an uncultured genus of filamentous organisms from activated sludge.

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Population dynamics of filamentous bacteria in Danish wastewater treatment plants with nutrient removal

Cited by 110 publications

References 62 publications

Overall functional gene diversity of microbial communities in three full-scale activated sludge bioreactors

Overall functional gene diversity of microbial communities in three full-scale activated sludge bioreactors

New automated procedure to determine the contribution of filamentous bacteria in activated sludge flocs

Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge

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