Population dynamics and distribution of hydrocarbon utilizing bacteria in Automobile workshops within Uyo metropolis, Akwa Ibom State

Ndubuisi-Nnaji, U. U.; John, Oum; Ofon, U. A.

doi:10.4314/jasem.v19i4.3

Cited by 2 publications

(5 citation statements)

References 9 publications

(10 reference statements)

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“…This indicated a high bacterial density in the sampled soils despite being contaminated with petroleum hydrocarbons. The result is similar to that of Ndubuisi-Nnaji [15], who analyzed soil samples of an automobile workshop in Uyo Metropolis, Nigeria. The findings of Akeredolu and Akinnibosun [16] have also reported a high bacterial load in soil contaminated with petroleum products in Benin City, Nigeria.…”

Section: Discussionsupporting

confidence: 84%

“…The occurrence of Bacillus spp. in this study corresponds with that of Ndubuisi-Nnaji [15] and Eric et al [19] who reported the identification of Bacillus spp. from oil polluted soil samples.…”

Section: B a B Asupporting

confidence: 91%

“…Despite recent advancements in biosurfactant science, it has remained difficult to obtain efficient biosurfactant-producing microbial strains. The existence of biosurfactant-producing bacteria and fungi in a variety of settings has been described in previous research [5,9,14,15,22]. All of the described species have been tested in the lab, however, the varying success rates due to biosurfactant generation have rendered much of the reported success more theoretical.…”

Section: Introductionmentioning

confidence: 96%

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Isolation and Screening of Biosurfactant-producing Bacteria from Hydrocarbon-contaminated Soil in Kano Metropolis, Nigeria

Fardami¹,

Kawo²,

Yahaya³

et al. 2022

J Biochem Microbiol Biotechnol

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Biosurfactants are surface-active biomolecules produced by microorganisms that have different applications in solving many environmental problems. This study was carried out to screen biosurfactant-producing bacteria isolated from hydrocarbon-contaminated soil of Kano Metropolis, Kano State- Nigeria. Soil samples were collected and processed. Biosurfactant-producing bacteria were enumerated, isolated and characterized using cultural, morphological and biochemical characteristics. Blood haemolysis, oil drop collapse and oil displacement tests were employed for the screening of the bacterial isolates for the potential to produce biosurfactant. The viable aerobic heterotrophic bacterial count of the samples ranges from 1.0 to 8.4×106 cfu/g. Eight bacterial genera were biochemically identified as Pseudomonas aeruginosa, Enterobacter cloacae, Pantoea agglomerans, Pseudomonas sp., Bacillus subtilis, Enterobacter alvei, Bacillus sp. and Klebsiella sp. Bacillus subtilis had the highest frequency of occurrence of 5(27%) while Bacillus sp. and Enterobacter alvei have the least occurrences of 1(6%) each. The eight identified bacterial isolates were all positive for the haemolysis test, drop collapse and oil displacement test.

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Section: Discussionsupporting

confidence: 84%

Section: B a B Asupporting

confidence: 91%

Section: Introductionmentioning

confidence: 96%

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Isolation and Screening of Biosurfactant-producing Bacteria from Hydrocarbon-contaminated Soil in Kano Metropolis, Nigeria

Fardami¹,

Kawo²,

Yahaya³

et al. 2022

J Biochem Microbiol Biotechnol

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“…reported that indiscriminate disposal and contamination of soil by used lubricating oil is prevalent in developing countries where there are no effective regulatory policies on the environment. According to the authors, oil spills from industries, filling stations and activities of automechanic workshops, all combine to contribute to soil contamination by hydrocarbons (Ndubuisi-Nnaji et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…In Nigeria, oil pollution problem has been prevalent since the commencement of oil exploration and establishment of the petroleum industry (Okoh et al, 2001). Different methods have been used in the biodegradation of oil polluted soils; some of these include the use of oil degrading microorganisms (Okwute et al, 2015, Ndubuisi-Nnaji et al, 2015, chicken droppings (Okwute and Ijah, 2014), melon shells, sawdust, rice husk, meshed groundnut husk (Stephen et al, 2013;Stephen and Temola, 2014).…”

Section: Introductionmentioning

confidence: 99%

Microbiological and physicochemical studies of spent lubricating oil contaminated soil amended with wood ash

Stephen¹,

Okwute²,

Peter³

et al. 2016

J. Biosci. Biotechnol. Discov.

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Microbiological and physicochemical studies of spent lubricating oil contaminated soil amended with wood ash (10% amendment level) were carried out for a period of 56 days. The aim of this study was to evaluate the effect of wood ash in conditioning spent lubricating oil contaminated soil. Serial dilution and pour plate methods were used in enumerating microbial growth. The pH, nitrate, moisture, phosphorus, organic matter content were also determined. The heterotrophic bacteria count ranged from 1.0×10 4 to 2.1×10 4 cfu/g for the oil free soil (OFS), 1.3×10 4 to 2.7×10 4 cfu/g for the polluted control soil (PCS) and 2.1×10 4 to 2.3×10 4 cfu/g for the amended soil (AS) while the fungal count ranged from 4.0×10 3 to 4.7×10 4 cfu/g for OFS, 4.0×10 3 to 8.2×10 4 cfu/g for PCS and 4.0×10 3 to 10.8×10 4 cfu/g for AS. Higher microbial counts were found on the PCS and AS compared to the OFS. There were no significant differences at 5% probability level between the treatments. The organisms isolated were species of Bacillus, Staphylococcus, Micrococcus, Aspergillus, Mucor, Penicillium and Saccharomyces. There were no significant differences (p>0.05) in the moisture content, nitrate, organic matter content and electrical conductivity. However, there were significant differences in the pH, organic carbon and phosphorus contents of the soil samples at 5% probability level. The results obtained in this study demonstrate the potential of wood ash to considerably increase the organic carbon, phosphorus and pH of the soil to slightly alkaline condition which favours the biodegradation of the spent lubricating oil contaminated soil.

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Population dynamics and distribution of hydrocarbon utilizing bacteria in Automobile workshops within Uyo metropolis, Akwa Ibom State

Cited by 2 publications

References 9 publications

Isolation and Screening of Biosurfactant-producing Bacteria from Hydrocarbon-contaminated Soil in Kano Metropolis, Nigeria

Isolation and Screening of Biosurfactant-producing Bacteria from Hydrocarbon-contaminated Soil in Kano Metropolis, Nigeria

Microbiological and physicochemical studies of spent lubricating oil contaminated soil amended with wood ash

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