Population characteristics of the factors of persistence ofEscherichia coli isolated from different ecological niches

Abstract

The range of antilysozyme activity (the sign of persistence) of Escheriehia coli clones from subpopulations isolated from natural and artificial ecosystems is established. It is shown that heterogeneity of the microorganism population reflects the ecological status of its habitat. Therefore, it can be used as a test-indicator in biomonitoring and as an index in the standardization of eubiotics. Key Words: E. coli; eubiotic; population analysis; persistenceThe population method has been widely used in epidemiology for prediction of epidemics [1], in experimental research for evaluation of persistence and elimination of the causative agent [5], and in environmental studies [6].The factors of microorganism persistence, which reflect the flexibility of the adaptation mechanisms of a given population, may be employed to solve ecological, hygienic, and biotechnological problems [2].The aim of this study was to explore the possibility of using the population method for assessing the habitat of microorganisms and for standardization of cultures used for the production of eubiotics. MATERIALS AND METHODSE. coli was selected because it is an ubiquitous microorganism with a high adaptive potential. Model subpopulations and subpopulations isolated from natural (human body and open water reservoir) and from man-made (sewers) ecosystems were used. All Department of Microorganism Persistence, Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy -of Sciences, Orenburg escherichia isolated from different econiches possessed typical biological characteristics. The enterobacteria were isolated from human intestine with due consideration for their intracellular parasitism [4]. E. coli serogroups 0-20, O-111, 0-75, O-128, and O-114 were isolated from patients with enteric infections. Typical E. coli whose serotype could not be determined with the standard diagnostic kit were isolated from normal subjects, from the Ural river at the site of water intake (clean zone) and waste disposal (contaminated zone), and from sewers.E. coli (strain M-17) contained in the preparation Colibacterin were employed as a model microorganism.The subpopulations were cloned by serial dilutions and inoculations, so that no more than 100 colonies grew in each dish. Population analysis of the antilysozyme activity (ALA) of E. coli was performed by the method of imprints [8]. Antilysozyme activity was assayed as described elsewhere [3]. RESULTSIn the subpopulation which was isolated from patients and consisted of E. coli of different sero-

Immunosupressive activity of opportunist pathogens of the infectious-inflammatory process in the urinary tract of men with calculous pyelonephritis

“…Встановлювалась частота наявності антикомплементарної активності таксонів, збудників інфекційно-запального процесу за концентрації 5, 10 і 20 СН50/мл [6]. Антилізоцимну активність збудників досліджували фотометричним методом [7]. Антиімуноглобулінову активність досліджували за методом, викладеним у роботі Гайдаш І.С.…”