Population based quantification of dendrites: evidence for the lack of microtubule-associate protein 2a,b in Purkinje cell spiny dendrites

Hamodeh, Salah; Eicke, Daniel; Napper, Ruth M. A.; Harvey, Richard F.; Sultan, Fahad

doi:10.1016/j.neuroscience.2010.08.021

Cited by 10 publications

(18 citation statements)

References 47 publications

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“…However, in 3D space, crossed branches of the same arbor did not contact each other, and, as could be readily visualized by rotating the PC, the small interbranch distances usually did not extend beyond the plane of the main dendritic trunk. Primary dendrites reportedly range from 4 to 6 μ m in thickness [11, 17] and from 8 to 9 μ m, including the 2–4 μ m-space between neighbors [46]. Our measurements of primary dendrite thickness agree well with those cited above (results not shown).…”

Section: Resultssupporting

confidence: 88%

“…This relatively large separation is because Bergmann glia, basket cells, and other interneurons need to be accommodated as well. In addition, PC dendritic diameters average 0.5–2.2 μ m for spiny distal dendrites and 3.45 μ m for smooth proximal dendrites [11]. Given these dimensions, with fine optical sectioning, it is fairly simple to resolve the dendrites of neighboring PCs.…”

Section: Methodsmentioning

confidence: 99%

“…Lastly, classical methods for tracing dendritic morphology are so time-consuming that it is impossible to reconstruct enough cells for appropriate analyses of dendritic morphologies across anatomical regions of interest. Newer methods are being developed [7–9], although few explore the local architecture of neuronal populations [10, 11] and their connectivity to different cell types [12, 13]. …”

Section: Introductionmentioning

confidence: 99%

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Comparative Morphology of Dendritic Arbors in Populations of Purkinje Cells in Mouse Sulcus and Apex

Nedelescu

Abdelhack

2013

Neural Plasticity

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Foliation divides the mammalian cerebellum into structurally distinct subdivisions, including the concave sulcus and the convex apex. Purkinje cell (PC) dendritic morphology varies between subdivisions and changes significantly ontogenetically. Since dendritic morphology both enables and limits sensory-motor circuit function, it is important to understand how neuronal architectures differ between brain regions. This study employed quantitative confocal microcopy to reconstruct dendritic arbors of cerebellar PCs expressing green fluorescent protein and compared arbor morphology between PCs of sulcus and apex in young and old mice. Arbors were digitized from high z-resolution (0.25 µm) image stacks using an adaptation of Neurolucida's (MBF Bioscience) continuous contour tracing tool, designed for drawing neuronal somata. Reconstructed morphologies reveal that dendritic arbors of sulcus and apex exhibit profound differences. In sulcus, 72% of the young PC population possesses two primary dendrites, whereas in apex, only 28% do. Spatial constraints in the young sulcus cause significantly more dendritic arbor overlap than in young apex, a distinction that disappears in adulthood. However, adult sulcus PC arbors develop a greater number of branch crossings. These results suggest developmental neuronal plasticity that enables cerebellar PCs to attain correct functional adult architecture under different spatial constraints.

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Section: Resultssupporting

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Morphology of Dendritic Arbors in Populations of Purkinje Cells in Mouse Sulcus and Apex

Nedelescu

Abdelhack

2013

Neural Plasticity

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“…The antibody was derived from hybridoma clone AP‐20 (Binder et al, ; Kalcheva et al, ) and recognizes the highly conserved region of human MAP2 between amino acids 995 and 1332 (Kalcheva et al, ). We have previously quantified the dendritic staining of this antibody in the cerebellar cortex and CA1 regions of rats (Hamodeh et al, ). The staining showed selective dendritic labeling of the Purkinje cells somata, primary and secondary dendrites, and the somata and dendrites of the molecular layer interneurons of the cerebellar cortex, as expected from previous descriptions (Binder et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…We have previously introduced an analysis of fibers based on a quantitative 3D immunohistochemical method (3D‐QIHC). By using microtubule‐associated protein (MAP2a,b) immunostaining of dendrites and confocal laser scanning microscopy, we reconstructed and analyzed quantitatively the dendrites of the cerebellar molecular layer (Hamodeh et al, ). This approach allowed us to calculate dendritic length density and average dendritic diameter rapidly enough to permit large sample analysis.…”

mentioning

confidence: 99%

Systematic analysis of neuronal wiring of the rodent deep cerebellar nuclei reveals differences reflecting adaptations at the neuronal circuit and internuclear levels

Hamodeh

Baizer²,

Sugihara

et al. 2014

J of Comparative Neurology

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A common view of the architecture of different brain regions is that, despite their heterogeneity, they have optimized their wiring schemes to make maximal use of space. Based on experimental findings, computational models have delineated how about two-thirds of the neuropil is filled out with dendrites and axons optimizing cable costs and conduction time while keeping the connectivity at the highest level. However, whether this assumption can be generalized to all brain regions has not yet been tested. Here we quantified and charted the components of the neuropil in the four deep cerebellar nuclei (DCN) of the rat's brain. We segmented and traced the neuropil stained with one of two antibodies, one antibody against dendritic microtubule-associated proteins (MAP2a,b) and the second against the Purkinje cell axons (PCP2). We compared fiber length density, average fiber diameter, and volume fraction within different components of the DCN in a random, systematic fashion. We observed differences in dendritic and axonal fiber length density, average fiber diameters, and volume fraction within the four different nuclei that make up the DCN. We observe a relative increase in the length density of dendrites and Purkinje cell axons in two of the DCN, namely, the posterior interposed nucleus and the lateral nucleus. Furthermore, the DCN have a surprisingly low volume fraction of their dendritic length density, which we propose is related to their special circuitry. In summary, our results show previously unappreciated functional adaptations among these nuclei.

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Methylmercury Neurotoxicity: Why Are some Cells more Vulnerable than Others?

Kaur

Aschner

Syversen

2012

Methylmercury and Neurotoxicity

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Population based quantification of dendrites: evidence for the lack of microtubule-associate protein 2a,b in Purkinje cell spiny dendrites

Cited by 10 publications

References 47 publications

Comparative Morphology of Dendritic Arbors in Populations of Purkinje Cells in Mouse Sulcus and Apex

Comparative Morphology of Dendritic Arbors in Populations of Purkinje Cells in Mouse Sulcus and Apex

Systematic analysis of neuronal wiring of the rodent deep cerebellar nuclei reveals differences reflecting adaptations at the neuronal circuit and internuclear levels

Methylmercury Neurotoxicity: Why Are some Cells more Vulnerable than Others?

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