Population-based pediatric reference intervals for general clinical chemistry analytes on the Abbott Architect ci8200 instrument

Ridefelt, Peter; Aldrimer, Mattias; Rödöö, Per-Olof; Niklasson, F; Jansson, Leif; Gustafsson, Jan Åke; Hellberg, Dan

doi:10.1515/cclm-2011-0787

Cited by 30 publications

(37 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, calculation of reference intervals revealed that several age and sex partitions were required to reflect minor fluctuations in electrolyte concentrations, i.e., chloride and sodium each required 4 age partitions, whereas potassium required 2. Although our data agree with the range of reference values that are typical for healthy individuals, the robust dataset demonstrates an even more complex pattern than previously observed (11)(12)(13). Another large study of Ͼ7000 Chinese adults aged 20 -79 years showed a similar electrolyte profile, in which at least 6 age partitions were required for electrolytes (14 ), further supporting the requirement of a large sample population to fully capture such minor fluctua- To maintain participant confidentiality (on the basis of Statistics Canada policy), each data point represents the mean of ≥11 closely associated data points.…”

Section: Discussionsupporting

confidence: 85%

“…Declining phosphate concentrations likely reflect both the cessation of bone growth after puberty and various stages of bone mineralization, as exemplified by the low values observed from 48 to 79 years of age. High total calcium and phosphate in childhood have previously been observed in the CALIPER study (1 ), and the downward trends with age are corroborated by other studies (13,15 ). Interestingly, the lower calcium and phosphate values observed for females have also been reported by some groups (11 ) but not others (14 ).…”

Section: Discussionsupporting

confidence: 76%

See 1 more Smart Citation

Biochemical Marker Reference Values across Pediatric, Adult, and Geriatric Ages: Establishment of Robust Pediatric and Adult Reference Intervals on the Basis of the Canadian Health Measures Survey

et al. 2015

View full text Add to dashboard Cite

BACKGROUND Biological covariates such as age and sex can markedly influence biochemical marker reference values, but no comprehensive study has examined such changes across pediatric, adult, and geriatric ages. The Canadian Health Measures Survey (CHMS) collected comprehensive nationwide health information and blood samples from children and adults in the household population and, in collaboration with the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER), examined biological changes in biochemical markers from pediatric to geriatric age, establishing a comprehensive reference interval database for routine disease biomarkers. METHODS The CHMS collected health information, physical measurements, and biosamples (blood and urine) from approximately 12 000 Canadians aged 3–79 years and measured 24 biochemical markers with the Ortho Vitros 5600 FS analyzer or a manual microplate. By use of CLSI C28-A3 guidelines, we determined age- and sex-specific reference intervals, including corresponding 90% CIs, on the basis of specific exclusion criteria. RESULTS Biochemical marker reference values exhibited dynamic changes from pediatric to geriatric age. Most biochemical markers required some combination of age and/or sex partitioning. Two or more age partitions were required for all analytes except bicarbonate, which remained constant throughout life. Additional sex partitioning was required for most biomarkers, except bicarbonate, total cholesterol, total protein, urine iodine, and potassium. CONCLUSIONS Understanding the fluctuations in biochemical markers over a wide age range provides important insight into biological processes and facilitates clinical application of biochemical markers to monitor manifestation of various disease states. The CHMS-CALIPER collaboration addresses this important evidence gap and allows the establishment of robust pediatric and adult reference intervals.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 76%

Biochemical Marker Reference Values across Pediatric, Adult, and Geriatric Ages: Establishment of Robust Pediatric and Adult Reference Intervals on the Basis of the Canadian Health Measures Survey

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Approximately 50 analytes were measured, and the results for the other analytes have been presented elsewhere [18,19,20,21]. The Regional Ethical Review Board of Uppsala (No.…”

Section: Methodsmentioning

confidence: 99%

“…The present study is part of a larger project [18] designed to define reference intervals for general clinical chemistry in healthy children 6 months to 18 years of age. The aim is to define the prevalence of elevated ALP in a population of apparently healthy children, and to calculate age- and gender-specific pediatric reference intervals for serum ALP.…”

Section: Introductionmentioning

confidence: 99%

Alkaline Phosphatase in Healthy Children: Reference Intervals and Prevalence of Elevated Levels

Ridefelt¹,

Gustafsson

Aldrimer

et al. 2014

Horm Res Paediatr

Self Cite

View full text Add to dashboard Cite

Background: Transient hyperphosphatasemia (TH) is an often unnoticed benign entity, primarily affecting children below 5 years of age. However, the prevalence among healthy children is unknown. We used data from a Swedish pediatric reference interval project to estimate the prevalence of high alkaline phosphatase (ALP) among healthy children and to calculate pediatric reference intervals. Methods: Blood was collected from 699 subjectively healthy children aged 6 months to 18 years. After exclusion of subjects with high ALP, age- and gender-specific reference intervals were calculated. Results: Six children had ALP levels >16.7 µkat/l (>1,000 U/l), including 4 females and 2 males aged 7-22 months. The prevalence in the age group from 6 months to 2 years was 6.2% (6/97). None of the older children had levels of ALP >16.7 µkat/l. The study did not include the follow-up of these apparently healthy children. Consequently, conditions others than TH explaining the elevated ALP could not be excluded. However, general chemistry analyses, such as liver enzymes, calcium, intact PTH and vitamin D, were essentially normal in these children. Conclusions: The prevalence of high ALP among subjectively healthy children was approximately 2.4% below 5 years of age and 6.2% below 2 years. Reference intervals vary with age and gender.

show abstract

“…Ovo je moguće pod uslovom da se i proizvođači opreme pridržavaju propisanih standarda, primenjuju savremene statističke metode i izvode multicentrične studije [6]. Danas se zajednički RV formiraju u određenom regionu jedne zemlje ili u nekoliko zemalja, dok se ostale laboratorije oslanjaju na ovako dobijene vrednosti [7,8].…”

Section: Discussionunclassified

Reference value parameters of complete blood count in children aging 6 and 7

Djelkapic¹,

Lesovic²

2016

Tim Med Glas

View full text Add to dashboard Cite

Sažetak:Nekada se za skup rezultata dobijenih testiranjem zdrave populacije koristio termin "normalne vrednosti". Napredak u tumačenju laboratorijskih nalaza je postignut upotrebom termina "referentne vrednosti" koji je precizniji i podrazumeva raspon od 95% vrednosti rezultata koji su dobijeni prilikom određivanja nekog parametra kod referentne populacije. Za pravilnu interpretaciju laboratorijskih nalaza neophodno je detaljno poznavati postupke i metode koji se koriste u laboratoriji, kao i uticaj pojedinih faktora na vrednosti rezultata. Poznato je da većina laboratorija u svetu koristi referentne vrednosti (RV) koje preporučuju proizvođači opreme, koje su već objavljene u literaturi ili koriste RV drugih laboratorija. Cilj našeg ispitivanja je bio da se odrede referentne vrednosti za parametre krvne slike koji odgovaraju uslovima rada u našoj laboratoriji. Istraživanje je trajalo 2 godine, obuhvatilo je 1248 zdrave dece (665 dečaka i 593 devojčice), starosti 6 i 7 godina. Za uzorkovanje venske krvi su korišćene vakutajner epruvete (Vacuette, Grenier-Bio-One, K2E, ljubičasti zapušač). Parametri krvne slike (KKS), leukociti ( RDW 10,12%). Poređenjem rezultata sa drugim laboratorijama došli smo do zaključka da se RV za pojedine parametre neznatno razlikuju, dok su kod drugih te razlike znatne, kao što je slučaj sa leukocitima kod kojih je gornja granica od 11,4 do 15,5 x 109/L. S obzirom na to da ovakve razlike mogu otežavati interpretaciju laboratorijskih nalaza, mišljenja smo da svaka laboratorija treba da odredi RV za svoje uslove rada. Klučne reči:referentne vrednosti, referentvi interval, referentni opseg, deca, pedijatrijski. Summary:The term "normal value" used to be applied for a set of results obtained by testing the health of population. The progress in the interpretation of laboratory results was achieved by using the term "reference value", which is more precise and includes a range of 95% of the results obtained in determining a parameter with the reference population. For the proper interpretation of laboratory findings, it is necessary to know in detail the procedures and methods used in laboratories, and the impact of certain factors on the value of the results. It is known that most laboratories in the world, use the reference value (RV) recommended by the manufacturers of equipment that have already been published in the literature or used as the RV of other laboratories. The aim of our study was to determine reference values for Complete Blood Count (CBC) parameters corresponding to the working conditions in our laboratory. The study lasted for 2 years, included 1.248 healthy children (665 boys and 593 girls), aging 6-7. For the sampling of venous blood vacutainer tubes (Vacuette, Grenier-Bio-One, K2E, purple stopper) were used. The parameters of the CBC leucocytes (WBC), erythrocytes (RBC), haemoglobin (Hgb), haematocrit (Hct), platelets (PLT) and erythrocyte indices (MCV, MCH, MCHC, RDW) were measured at HmX haematology analyzer (Beckman-Coulter). Reference limits were defined as the mean ...

show abstract

Population-based pediatric reference intervals for general clinical chemistry analytes on the Abbott Architect ci8200 instrument

Abstract: The data generated is primarily applicable to a Caucasian population when using the Abbott Architect platform, but could be used by any laboratory if validated for the local patient population.

Cited by 30 publications

References 23 publications

Biochemical Marker Reference Values across Pediatric, Adult, and Geriatric Ages: Establishment of Robust Pediatric and Adult Reference Intervals on the Basis of the Canadian Health Measures Survey

Biochemical Marker Reference Values across Pediatric, Adult, and Geriatric Ages: Establishment of Robust Pediatric and Adult Reference Intervals on the Basis of the Canadian Health Measures Survey

Alkaline Phosphatase in Healthy Children: Reference Intervals and Prevalence of Elevated Levels

Reference value parameters of complete blood count in children aging 6 and 7

Contact Info

Product

Resources

About