Population and genetic diversity of Tibetan red deer based on fecal DNA

Hu, Hejiao; Xing, B.; Yang, Minghao; Mpemba, Hamenya; Lv, Zhonghai; Zhang, Minghai

doi:10.1007/s11676-017-0419-2

Cited by 4 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on published studies on red deer, eight pairs of microsatellite primers (ETH225, T501, T156, BM848, T530, DM45, N, T507) were used for individual identification [ 27 , 28 , 29 ]. The 5′ end of the upstream primer in each microsatellite locus was fluorescently labeled ( Table S2 ).…”

Section: Methodsmentioning

confidence: 99%

The Effect of Landscape Environmental Factors on Gene Flow of Red Deer (Cervus canadensis xanthopygus) in the Southern of the Greater Khingan Mountains, China

Guo

et al. 2023

Biology

Self Cite

View full text Add to dashboard Cite

Red deer (Cervus canadensis xanthopygus) living in the north of China are restricted and threatened due to human activities and the changes in the natural environment, which influence the dispersal and effective gene flow between different groups of red deer. Effective gene flow plays an important role in maintaining genetic diversity and structure and ensuring population health. In order to evaluate the genetic diversity level and understand the gene flow between different red deer groups, 231 fresh fecal samples were collected from the southern part of the Greater Khingan Mountains, China. A microsatellite marker was used for genetic analysis. The results showed that the genetic diversity of red deer was intermediate in this region. Significant genetic differentiation among different groups was found in the main distribution area (p < 0.01) using F-statistics and the program STRUCTURE. Different degrees of gene flow existed in red deer groups, and the roads (importance = 40.9), elevation (importance = 38.6), and settlements (importance = 14.1) exerted main effects on gene flow between red deer groups. Human-made factors should be noticed and strictly supervised in this region to avoid excessive disturbance to the normal movement of the red deer. Further conservation and management of red deer should reduce the intensity of vehicular traffic in the concentrated distribution areas of red deer, especially during the heat season. This research helps us better understand the genetic level and health status of red deer in the southern part of the Greater Khingan Mountains and provides theoretical references for protecting and restoring the red deer populations in China.

show abstract

Section: Methodsmentioning

confidence: 99%

The Effect of Landscape Environmental Factors on Gene Flow of Red Deer (Cervus canadensis xanthopygus) in the Southern of the Greater Khingan Mountains, China

Guo

et al. 2023

Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…We repeated the sampling route marked by red cloth strips to collect fecal pellets from 20 to 27 December in 2016. When we encountered newly left feces along transects marked by a red cloth strip and GIS, we sampled, transported and stored them by the same method [19,41,42].…”

Section: Sampling Designmentioning

confidence: 99%

“…So far, there is a lack of strong evidence to explain the relationship between the number of footprint trails and the number of roe deer individuals. In terms of the area used to convert abundance to density, the current studies in China estimated the density by dividing the population size by the whole study area instead of the effective area [12,31,42]. In general, the effective area is always smaller than the whole study area, meaning that using the whole area is still very likely to underestimate the deer density to some degree.…”

Section: Population Abundance and Densitymentioning

confidence: 99%

Estimating Abundance of Siberian Roe Deer Using Fecal-DNA Capture-Mark-Recapture in Northeast China

Chen

et al. 2020

Animals

View full text Add to dashboard Cite

It is necessary to estimate the population abundance of deer for managing their populations. However, most estimates are from high-density populations inhabiting the forests of North America or Europe; there is currently a lack of necessary knowledge regarding low-density deer populations in different forest habitats. In this article, we used fecal DNA based on the capture-mark-recapture method to estimate the population abundance of Siberian roe deer (Capreolus pygargus) in Liangshui National Nature Reserve in the Lesser Xing’an Mountains, northeast China, where the deer population was found to be of a low density by limited studies. We used a robust survey design to collect 422 fecal pellet groups in 2016 and extracted DNA from those samples, generating 265 different genotypes; we thus identified 77 deer individuals based on six microsatellite markers (Roe1, Roe8, Roe9, BM757, MB25 and OarFCB304). With capture and recapture records of these 77 individuals, the abundance of roe deer was estimated to be 87 deer (80–112, 95% CI) using the Program CAPTURE. Using an effective sampling area which resulted from the mean maximum recapture distance (MMRD), we converted the population abundance to a density of 2.9 deer/km2 (2.7–3.7, 95% CI). Our study estimated the roe deer population abundance by a feces-based capture-mark-recapture approach in northeast China, successfully demonstrating the applicability of non-invasive genetic sampling in monitoring populations of deer in this area, which contributes to the development of low-density deer population ecology and management.

show abstract

“…Microsatellite DNA marker is widely used in research of animal genetic diversity due to its unique characteristics (wide distribution, rich polymorphism, codominant inheritance, and rapid detection). At present, the application of microsatellites in cervid animals mainly includes sika deer, red deer, and black tail deer (Brinkman et al, 2010;Hu et al, 2018;Lv et al, 2014;Talbot et al, 1996). However, the research on whitered deer is still limited to describing its location and apparent characteristics, which have not been reported at the molecular level, such as the research on the genetic relationships and genetic diversity among 28 individuals in the white-red deer population.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis of the Only White-Red Deer Population in China

Han¹

2023

PJZ

View full text Add to dashboard Cite

The white-red deer population in China is composed of 28 individuals introduced from New Zealand. However, the genetic relationships between these individuals is unknown. The aim of the present study was to determine the genetic diversity and genetic relationship in the white-red deer population. We used ten microsatellite loci to genotype all 28 white-red deer individuals in China, and constructed a Neighborjoining (NJ) tree based on genetic distances. In addition, genetic structure analysis was carried out. The results showed that a total of 67 alleles were detected, and the average number of alleles at each locus was 6.7. There was high genetic diversity but severe inbreeding in the white-red deer population, and there is no father-mother-offspring relationship or father-offspring relationship in white-red deer population. Our study provides the essential data support and reference for the conservation and sustainable development of the domestic white-red deer population in China.

show abstract

Population and genetic diversity of Tibetan red deer based on fecal DNA

Cited by 4 publications

References 15 publications

The Effect of Landscape Environmental Factors on Gene Flow of Red Deer (Cervus canadensis xanthopygus) in the Southern of the Greater Khingan Mountains, China

The Effect of Landscape Environmental Factors on Gene Flow of Red Deer (Cervus canadensis xanthopygus) in the Southern of the Greater Khingan Mountains, China

Estimating Abundance of Siberian Roe Deer Using Fecal-DNA Capture-Mark-Recapture in Northeast China

Genetic Diversity Analysis of the Only White-Red Deer Population in China

Contact Info

Product

Resources

About