Population analysis of Vibrio parahaemolyticus originating from different geographical regions demonstrates a high genetic diversity

Urmersbach, Sara; Alter, Thomas; Koralage, Madura Sanjeevani Gonsal; Sperling, L.; Gerdts, Gunnar; Messelhäußer, Ute; Huehn, Stephan

doi:10.1186/1471-2180-14-59

Cited by 23 publications

(20 citation statements)

References 46 publications

(106 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MLST method showed 76 STs, of which 51 (67.1%) STs were newly identified. Similar results were reported by Urmersbach et al (27), where 130 V. parahaemolyticus strains obtained from a marine environment were classified into 82 STs, and 82.9% of them were described as new. Rahman et al (28) also identified 63 unique STs in the isolates tested, and 49 (77.8%) STs were not described in the database.…”

Section: Discussionsupporting

confidence: 89%

“…It was also found that the largest PFGE 60 group, with 7 strains recovered mainly from clams in Italy, was resistant to ampicillin and streptomycin and belonged to ST481. This sequence type was previously identified in environmental isolates from Germany and Italy, as well as in clinical strains from China (27,28) (http://pubmlst.org/vparahaemolyticus). In the present study, ST481 was closely related to new ST1359 and ST1389 detected in V. parahaemolyticus from clams originating from Italy and the Netherlands, respectively.…”

Section: Discussionmentioning

confidence: 54%

See 1 more Smart Citation

Antimicrobial Resistance, Virulence Factors, and Genetic Profiles of Vibrio parahaemolyticus from Seafood

Lopatek

Wieczorek

Osek

2018

Appl Environ Microbiol

View full text Add to dashboard Cite

is a widespread bacterium in the marine environment and is responsible for gastroenteritis in humans. Foodborne infections are mainly associated with the consumption of contaminated raw or undercooked fish and shellfish. The aim of this study was to determine the antimicrobial resistance, virulence factors, and genetic profiles of isolates from seafood originating from different countries. A total of 104 (17.5%) isolates were recovered from 595 analyzed samples. The isolates were tested for the presence of the and genes, involved in the pathogenesis of infections in humans, and these genes were detected in 3 (2.9%) and 11 (10.6%) isolates, respectively. The -positive isolates also possessed the gene, which is responsible for urease production. Moreover, the activity of protease A was identified in all strains. Antimicrobial resistance revealed that most isolates were resistant to ampicillin (75.0%) and streptomycin (68.3%), whereas all strains were sensitive to chloramphenicol and tetracyclines. Most of the isolates (55.8%) showed resistance against two classes of antimicrobials, mainly to ampicillin and streptomycin (46.2%). Only one isolate displayed a multiresistant pattern. Genotypic analysis of revealed a high degree of diversity among the isolates tested. The pulsed-field gel electrophoresis (PFGE) method distinguished 73 clonal groups, and the most numerous group consisted of 7 strains. Sequencing by the multilocus sequence typing (MLST) method showed 76 sequence types (STs), of which ST481 and ST1361 were most frequently identified. In addition, 51 (67.1%) new sequence types were discovered and added to the PubMLST international database. The presence of in seafood may pose a risk for consumers, especially in countries where shellfish are eaten raw. In recent years, a significant increase of food poisoning caused by these bacteria has been also observed in Europe. Our results highlight the high level of contamination of seafood, along with the isolates being potentially pathogenic for humans. However, the first-line antimicrobials, such as tetracyclines and fluoroquinolones, remained highly effective against The monitoring of antimicrobial resistance of isolates is important to ensure the high efficacy in the treatment of human infections. Most of strains possessed new sequence types (STs), which showed the high genetic diversity of the isolates tested.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 54%

Antimicrobial Resistance, Virulence Factors, and Genetic Profiles of Vibrio parahaemolyticus from Seafood

Lopatek

Wieczorek

Osek

2018

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…So translating the in-frame nucleotide sequences into peptide sequences allows a phylogenetic analysis based on pSTs. In this study, the assignment of pSTs of the analyzed isolates to CCs was carried out as previously (Theethakaew et al, 2013 ; Urmersbach et al, 2014 ) and predicted also by goeBURST algorithm.…”

Section: Methodsmentioning

confidence: 99%

Prevalence and genetic diversity of clinical Vibrio parahaemolyticus isolates from China, revealed by multilocus sequence typing scheme

et al. 2015

View full text Add to dashboard Cite

The population structure of clinical Vibrio parahaemolyticus isolates spreading in China remains undefined. We brought 218 clinical isolates from the pubMLST database originating from different regions of China collected since the year of 1990, analyzed by multilocus sequence typing (MLST), to elucidate the prevalence and genetic diversity of V. parahaemolyticus circulating in Chinese population. The MLST scheme produced 137 sequence types (STs). These STs were clustered into six clonal complexes (CCs), six doublets, and 91 singletons, exhibiting a high level of genetic diversity. However, less diversity was displayed on the peptide level: only 46 different peptide sequence type (pST) were generated, with pST2 (44.0%, 96/218) and pST1 (15.1%, 33/218) the predominant. Further analysis confirmed all the pSTs belong to a single complex founded by pST1, pST2, pST3, and pST4. recA presented the highest degree of nucleotide diversity (0.026) and the largest number of variable sites (176) on the nucleotide level. pyrC was the most diverse locus on the peptide level, possessing the highest percentage of variable sites (9.2%, 15/163). Significant linkage disequilibrium with the alleles was detected when the Standardized Index of Association (ISA) was calculated both for the entire isolates collection (0.7169, P < 0.01) and for the 137 STs (ISA = 0.2648, P < 0.01). In conclusion, we provide an overview of prevalence and genetic diversity of clinical V. parahaemolyticus spreading in Chinese population using MLST analysis. The results would offer genetic evidences for uncovering the microevolution relationship of V. parahaemolyticus populations.

show abstract

“…In the ME tree ( Fig. 2 ) published trh2 variants of Norway [ 36 ] and some more German isolates with trh genes [ 39 ] are displayed. Few trh negative strains with ST 121 and ST 761 were incorporated into the ME tree.…”

Section: Discussionmentioning

confidence: 99%

Characterization of trh2 Harbouring Vibrio parahaemolyticus Strains Isolated in Germany

et al. 2015

View full text Add to dashboard Cite

Background Vibrio parahaemolyticus is a recognized human enteropathogen. Thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) as well as the type III secretion system 2 (T3SS2) are considered as major virulence factors. As tdh positive strains are not detected in coastal waters of Germany, we focused on the characterization of trh positive strains, which were isolated from mussels, seawater and patients in Germany.ResultsTen trh harbouring V. parahaemolyticus strains from Germany were compared to twenty-one trh positive strains from other countries. The complete trh sequences revealed clustering into three different types: trh1 and trh2 genes and a pseudogene Ψtrh. All German isolates possessed alleles of the trh2 gene. MLST analysis indicated a close relationship to Norwegian isolates suggesting that these strains belong to the autochthonous microflora of Northern Europe seawaters. Strains carrying the pseudogene Ψtrh were negative for T3SS2β effector vopC. Transcription of trh and vopC genes was analyzed under different growth conditions. Trh2 gene expression was not altered by bile while trh1 genes were inducible. VopC could be induced by urea in trh2 bearing strains. Most trh1 carrying strains were hemolytic against sheep erythrocytes while all trh2 positive strains did not show any hemolytic activity. TRH variants were synthesized in a prokaryotic cell-free system and their hemolytic activity was analyzed. TRH1 was active against sheep erythrocytes while TRH2 variants were not active at all.ConclusionOur study reveals a high diversity among trh positive V. parahaemolyticus strains. The function of TRH2 hemolysins and the role of the pseudogene Ψtrh as pathogenicity factors are questionable. To assess the pathogenic potential of V. parahaemolyticus strains a differentiation of trh variants and the detection of T3SS2β components like vopC would improve the V. parahaemolyticus diagnostics and could lead to a refinement of the risk assessment in food analyses and clinical diagnostics.

show abstract

Population analysis of Vibrio parahaemolyticus originating from different geographical regions demonstrates a high genetic diversity

Cited by 23 publications

References 46 publications

Antimicrobial Resistance, Virulence Factors, and Genetic Profiles of Vibrio parahaemolyticus from Seafood

Antimicrobial Resistance, Virulence Factors, and Genetic Profiles of Vibrio parahaemolyticus from Seafood

Prevalence and genetic diversity of clinical Vibrio parahaemolyticus isolates from China, revealed by multilocus sequence typing scheme

Characterization of trh2 Harbouring Vibrio parahaemolyticus Strains Isolated in Germany

Contact Info

Product

Resources

About