Poplar Bark Storage Protein and a Related Wound-Induced Gene Are Differentially Induced by Nitrogen

Coleman, Gary D.; Bañados, M. Pilar; Chen, T. H. H.

doi:10.1104/pp.106.1.211

Cited by 52 publications

(39 citation statements)

References 20 publications

(32 reference statements)

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“…Several environmental factors, among which photoperiod (Coleman et al 1991;Langheinrich & Tishner 1991), nitrogen availability (van Cleve & Apel 1993; Coleman et al 1994), temperature (van Cleve & Apel 1993) and wounding (Davis et al 1993) influence BSP expression. However, the molecular mechanisms controlling BSP expression are not well characterized.…”

Section: Remaining Five Spots As Shown Inmentioning

confidence: 99%

Unravelling the response of poplar (Populus nigra) roots to mechanical stress imposed by bending

Scippa

Trupiano

Rocco

et al. 2008

Plant Biosystems - An International Journal Dealing with all As

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Mechanical stress is a widespread environmental condition that can be caused by several factors (i.e. gravity, touch, wind, soil density, soil compaction and grazing, slope) and that can severely affect plant stability. In response to mechanical stress and to improve their anchorage, plants have developed complex mechanisms to detect mechanical perturbation and to induce a suite of modifications at anatomical, physiological, biochemical, biophysical and molecular level. Although it is well recognized that one of the primary functions of root systems is to anchor the plant to the soil, root response to mechanical stresses have been investigated mainly at morphological and biomechanical level, whereas investigations about the molecular mechanisms underlying these important alterations are still in an initial stage. We have used an experimental system in which the taproot poplar seedlings are bent to simulate mechanical perturbation to begin investigate the mechanisms involved in root response to mechanical stress. The results reported herein show that, in response to bending, the poplar root changes its morphology by emitting new lateral roots, and its biomechanical properties by increasing the root biomass and lignin synthesis. In addition, using a proteomic approach, we found that several proteins involved in the signal transduction pathway, detoxification and metabolism are up-regulated and/or down-regulated in the bent root. These results provide new insight into the obscure field of woody root response to mechanical stress, and can serve as a basis for future investigations aimed at unravelling the complex mechanism involved in the reaction of root biology to environmental stress.

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Section: Remaining Five Spots As Shown Inmentioning

confidence: 99%

Unravelling the response of poplar (Populus nigra) roots to mechanical stress imposed by bending

Scippa

Trupiano

Rocco

et al. 2008

Plant Biosystems - An International Journal Dealing with all As

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“…In general, bark storage proteins accumulate in the bark of many woody plants during autumn and winter and their expression is controlled by photoperiod (Coleman et al 1991(Coleman et al , 1992. Poplar bark storage proteins have been proven to be associated with short-day photoperiods and induced by nitrogen and are active in floral tissues (Coleman et al 1994;Zhu and Coleman 2001). NADP-dependent malic enzymes provide high CO 2 concentrations for Rubisco fixation in the C 4 leaf chloroplasts (Estavillo and Rao 2007).…”

Section: Discussionmentioning

confidence: 97%

Genes expressed in Taiwania reproductive organs include two male-cone protein 1 precursor homologs

Chu

Shen

Lee

et al. 2011

J. Plant Biochem. Biotechnol.

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Taiwania (Taiwania cryptomerioides Hayata), a conifer native to Taiwan, is an economically important tree species due to its high resistance to insect infection and high wood utilization properties; however, when it was grown in clonal orchards, no filled seeds or fertile pollen were obtained. To address this reproductive dilemma, a suppressive subtractive hybridization (SSH) library was constructed from male cones, female cones and leaves. Reverse transcription-polymerase chain reaction (RT-PCR) analysis confirmed that two bark storage proteins, a betaphellandrene synthase, an endochitinase A2 precursor and two male-cone protein 1 precursors were specifically expressed in the reproductive organs of Taiwania. Comparisons of these two male-cone protein 1 precursors with those of Pinus radiata suggest that these genes may have different functions during different stages of male cone development and that different specific genes may be obtained from male cones collected at different stages of development.

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“…In a number of woody perennial species, organic nitrogen is stored in specialized tissues such as bark, to be further remobilized for the formation of developing organs in periods of active growth (Coleman et al 1994). In woody perennial plants, the reaction catalyzed by cytosolic glutamine synthetase (GS1) may be a major step in controlling plant growth and development because it combines the re-assimilation with transport of nitrogen released in metabolic processes (Sua´rez et al 2002).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, much less is known about the characteristics of GS in woody plants, particularly gymnosperms, despite their economic and ecological importance. Woody perennial plants are a unique model with which to study ammonium assimilation since, in many woody species, N assimilated from the soil is not used immediately but is stored in the bark to be further remobilized the next growing season for the formation of new developing organs (Coleman et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of the 5'-upstream region of a gibberellin-inducible cytosolic glutamine synthetase gene ( GS1b ) expressed in pine vascular tissue

Gómez‐Maldonado

Cánovas

Ávila

2004

Planta

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The promoter region of the cytosolic glutamine synthetase GS1b gene was isolated from the conifer Pinus sylvestris L. (Scots pine). The 1,171-bp stretch of sequence lying immediately upstream of the transcriptional start site was sufficient to drive the expression of a reporter gene in a manner consistent with the expression pattern of the native GS1b gene. Computer analysis of putative cis elements in this promoter region revealed the presence of an AT box, an AC motif similar to those found in other genes expressed in the vascular tissue, and a gibberellin (GA)-responsive element. Consistent with the latter finding, GS1b gene expression was induced by exogenously supplied gibberellic acid (GA3) in germinating pine embryos and pine seedlings. In order to examine if the putative GA-response element found in the GS1b promoter could function in the regulation of GS1b expression, a series of deletions of the upstream gene region were fused to the uidA reporter gene, and transient expression analyzed either in untreated or in GA3-treated pine (Pinus pinaster Ait.) protoplasts. Deletion analysis revealed that sequences containing the GA-responsive element, located between -1005 and -724 bp were essential for the increased promoter activity observed in response to GA3. Furthermore, electrophoretic-mobility-shift assays showed that pine nuclear proteins bind to a 22-bp sequence that contains the GA-response element, located between -768 and -747 bp relative to the transcription start site.

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Poplar Bark Storage Protein and a Related Wound-Induced Gene Are Differentially Induced by Nitrogen

Cited by 52 publications

References 20 publications

Unravelling the response of poplar (Populus nigra) roots to mechanical stress imposed by bending

Unravelling the response of poplar (Populus nigra) roots to mechanical stress imposed by bending

Genes expressed in Taiwania reproductive organs include two male-cone protein 1 precursor homologs

Molecular analysis of the 5'-upstream region of a gibberellin-inducible cytosolic glutamine synthetase gene ( GS1b ) expressed in pine vascular tissue

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