Poor-quality red blood cell labelling with technetium-99m: case report and review of the literature

Adalet, İşık; Cantez, Sema

doi:10.1007/bf00175767

Cited by 7 publications

References 7 publications

(7 reference statements)

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First evaluation of technetium-99m dimercaptopropionyl albumin as a possible tracer agent for ventriculography in a volunteer

Verbeke

Vanhecke²,

Mortelmans³

et al. 1994

Eur J Nucl Med

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Technetium-99m labelled red blood cells (99mTc-RBCs) are the standard radiopharmaceutical for radionuclide ventriculography but suffer from some practical disadvantages such as risk of viral contamination and lengthy preparation (in vitro labelling) or poor labelling efficiency due to patient medication interactions (in vivo labelling). 99mTc-labelled human serum albumin (HSA) is not really a valuable alternative as the activity diffuses too rapidly out of the vascular space due to the weak binding of the radionuclide. We have modified HSA by reaction with N-hydroxysuccinimidyl 2,3-di(S-acetylmercapto)propionate (SAMP) to introduce a varying number of 2,3-dimercaptopropionyl (DMP) side chains. The resulting DMP-HSA can be rapidly labelled with 99mTc at room temperature by simple addition of stannous ions and eluate of a 99mTc generator. After evaluation in mice and rabbits, two different 99mTc-DMP-HSA preparations - obtained after reaction of SAMP with albumin in a molar ratio of, respectively, 8:1 and 16:1 - were tested in a volunteer and compared to 99mTc-RBCs. The blood time-activity curves of the three preparations were quite similar but both 99mTc-DMP-HSA preparations were excreted much less into the urine than 99mTc-RBCs. Ventriculography was performed with the three tracer agents, each time with a 1-week interval. In the three studies, the heart was clearly visualized and the left and right ventricle could be easily delineated. The ejection fractions obtained after administration of the three preparations were similar. With both 99mTc-DMP-HSA preparations the low activity in the spleen was a distinct advantage and facilitated delineation of the left ventricle.(ABSTRACT TRUNCATED AT 250 WORDS)

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First evaluation of technetium-99m dimercaptopropionyl albumin as a possible tracer agent for ventriculography in a volunteer

Verbeke

Vanhecke²,

Mortelmans³

et al. 1994

Eur J Nucl Med

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Failure of in vivo labelling of erythrocytes with technetium-99m: It is not Teflon that is the problem but rather the injection technique

Verbeke

Verbruggen

1997

Eur J Nucl Med

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Failure to label red blood cells adequately in daily practice using an in vivo method: methodological and clinical considerations

et al. 1995

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This study was conducted to evaluate the frequency and possible causes of poor red blood cell (RBC) labelling when performing equilibrium gated blood pool (GBP) radionuclide angiography at rest with an in vivo method. The influence of the mode of administration on tagging efficiency was studied by investigating the image quality in 160 patients referred for evaluation of left ventricular (LV) function prior to or after coronary angiography, while using a roughly standardized administration protocol. The patients were subclassified into four groups according to the way both molecules involved in the tagging procedure were administered. When poor image quality was found (in 9.4% of the patients), the labelling efficiency was quantified and the frequency of failed tagging in each group was calculated. A significant association was found between poor labelling and the use of a Teflon catheter or butterfly needle for the injection of the stannous agent. In another 737 patients, in order to avoid the problems observed in the first group, a strict administration protocol was applied to analyse the frequency of poor tagging and its possible causes. Suboptimal image quality was present in 88 patients (11.9%). Quantitatively confirmed poor tagging was present in 36 of the 88 (40.9%, or 4.9% of the whole group); the remaining 52 patients showed borderline normal labelling (> 80% bound fraction). Drug interference was studied by comparing the medications used by the 36 patients showing poor binding with those used by 44 control patients. A significant relationship was found between the use of heparin or chemotherapy and the tagging. The influence of several clinical factors on the labelling was also investigated.(ABSTRACT TRUNCATED AT 250 WORDS)

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Poor-quality red blood cell labelling with technetium-99m: case report and review of the literature

Cited by 7 publications

References 7 publications

First evaluation of technetium-99m dimercaptopropionyl albumin as a possible tracer agent for ventriculography in a volunteer

First evaluation of technetium-99m dimercaptopropionyl albumin as a possible tracer agent for ventriculography in a volunteer

Failure of in vivo labelling of erythrocytes with technetium-99m: It is not Teflon that is the problem but rather the injection technique

Failure to label red blood cells adequately in daily practice using an in vivo method: methodological and clinical considerations

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