Poor intracytoplasmic sperm injection outcome in infertile males with azoospermia factor c microdeletions

Zhang, Li; Mao, Jiaming; Li, Ming; Lian, Ying; Lin, Shengli; Chen, Lixue; Yan, Liying; Qiao, Jie; Liu, Ping

doi:10.1016/j.fertnstert.2021.01.025

Cited by 15 publications

(17 citation statements)

References 35 publications

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“…Liu reported that fertilization competent, viable embryo rate, and pregnancy rate of spermatozoa retrieved from men with Y chromosome AZFc chromosome deletions were similar to men without it ( 27 ). Our results showed that lower day 3 oocytes utilization rate and high-score embryo rate and lower cumulative CPR and cumulative LBR were observed in patients with Y chromosome AZFc microdeletion, which are similar to Zhang`s report but with less heterogeneity, because the objects we included were all testicular sperm by micro-TESE ( 28 ). These results are also consistent with the finding reported by Van and the primary function of the AZFc region in the Y chromosome is involvement in spermatozoa quality or function than in spermatogenesis ( 29 ).…”

Section: Discussionsupporting

confidence: 88%

Clinical Outcomes and Live Birth Rate Resulted From Microdissection Testicular Sperm Extraction With ICSI-IVF in Non-Obstructive Azoospermia: A Single-Center Cohort Study

Zheng

et al. 2022

Front. Endocrinol.

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BackgroundMost of data available in the literature reported the sperm retrieval rate and limited intracytoplasmic sperm injection (ICSI) results of microdissection testicular sperm extraction (micro-TESE) in non-obstructive azoospermia (NOA) patients with different etiologies. Unfortunately, there is currently a lack of comprehensive data to guide clinicians in conducting comprehensive consultations with NOA patients.ObjectivesTo obtain more comprehensive evidence-based data and clinical outcomes for better consultation of NOA patients who opted to undergo micro-TESE combined with ICSI-IVFMethodsIt was a retrospective study involved 968 NOA patients underwent micro-TESE during January 2015 to December 2019. Embryological, clinical, and live birth outcomes were demonstrated comprehensively and three kinds of stratification analyses were performed based on ICSI-IVF cycles using frozen and fresh sperm, different etiologies of NOA and various amounts of sperm retrieved.ResultsThe sperm retrieval rate was 44.6%, and ICSI was performed in 299 couples leading to 150 clinical pregnancies and 140 live-birth deliveries. The clinical pregnancy rate (CPR) was 50.17%, and the cumulative live birth rate (LBR) was 46.82%, and the low birth defects rate was 1.43%. No significant difference was observed about cumulative LBR in frozen sperm group and fresh sperm group (47.5% vs 42.9%, P>0.05). NOA patients with AZFc microdeletions had the lowest rate of a high-score embryo on day 3 (4.4%, P<0.05) and the lowest cumulative LBR (19.4%, P<0.05). NOA patients with lower sperm count (having fewer than 20 sperms retrieved) had significantly lower cumulative LBR than those with higher sperm count (having more than 20 sperms retrieved) (28.1% vs 51.9%, P<0.05).ConclusionsFor those NOA patients who stepped in ICSI-IVF cycles, the cumulative LBR was 46.82%. No significant difference was indicated in the LBR between ICSI-IVF cycles using frozen or fresh testicular sperm. Compared to other etiologies, NOA caused by AZFc microdeletions have the poorest embryological and clinical outcomes. Patients with less testicular sperm retrieved have poorer embryological and clinical outcomes.

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Section: Discussionsupporting

confidence: 88%

Clinical Outcomes and Live Birth Rate Resulted From Microdissection Testicular Sperm Extraction With ICSI-IVF in Non-Obstructive Azoospermia: A Single-Center Cohort Study

Zheng

et al. 2022

Front. Endocrinol.

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“…Previous studies have reported that inflammation and congenital factors may influence ICSI outcomes [39,40], while other factors such as surgery do not affect ICSI outcomes [10,15,31]. Therefore, while there were significant differences in female and male BMI and causes of OA, we contained BMI and congenital factors (the absence or dysplasia of seminal vesicles) in our linear and logistic regression analyses in order to manage possible bias [29,32]. The HQER for the PESA group, regardless of inflammation status, was higher than for the TESA group.…”

Section: Discussionmentioning

confidence: 99%

“…Oocyte retrieval was performed 34–36 h after human chorionic gonadotrophin injection, and luteal phase support was started and continued until 10 weeks of gestation. Cleaved embryos on day 3 of embryo culture or blastocysts on day 5 were transferred according to the scores by checking cell number, fragmentation, and cell symmetry [ 29 , 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…The secondary outcome measures included fertilization rate, high-quality embryo rate (HQER), implantation rate, no embryo suitable for transfer cycles rate, miscarriage rate, birth weight, and congenital anomaly birth rate. The calculations of ICSI outcomes were as described previously [ 29 ]. The biochemical pregnancy rate was defined as the number of biochemical pregnancies divided by the number of embryo transfer cycles, the clinical pregnancy rate was defined as the number of clinical pregnancies divided by the number of embryo transfer cycles, and LBDR was defined as the percentage of live birth delivery cycles out of the total number of embryo transfer cycles.…”

Section: Methodsmentioning

confidence: 99%

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Does ICSI outcome in obstructive azoospermia differ according to the origin of retrieved spermatozoa or the cause of epididymal obstruction? A comparative study

Yuan

et al. 2022

Int Urol Nephrol

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Purpose To determine whether ICSI outcomes are affected by sperm source or genital tract inflammatory status. Methods A retrospective cohort study was conducted in all consecutive obstructive azoospermia patients who underwent testicular sperm aspiration (TESA) or percutaneous epididymal sperm aspiration (PESA) and ICSI between February 1, 2017, and December 31, 2020. Couples were excluded if they were diagnosed with monogenic disease, abnormal karyotype, or had female uterine malformation. The primary objective was to determine whether ICSI outcomes are affected by the use of testicular or epididymal spermatozoa, and the secondary objective was to explore the effect of granulocyte elastase on ICSI outcomes using epididymal spermatozoa. Results Compared with TESA, inflammatory and non-inflammatory PESA patients exhibited a better high-quality embryo rate, with significant differences among the three groups (49.43 vs. 55.39% and 56.03%; odds ratio, 6.345 and 6.631; 95% confidence interval, 0.340–12.350, and 1.712–11.550; P = 0.038 and P = 0.008, respectively). The fertilization rate, clinical pregnancy rate, live birth delivery rate, and congenital anomaly birth rate were similar in patients who underwent TESA or PESA (with or without inflammation). Conclusions The high-quality embryo rate in PESA patients was higher than that in TESA patients. After successful pregnancy, ICSI outcomes did not differ between patients with obstructive azoospermia who experienced TESA or PESA and those with or without genital tract inflammation.

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“…According to the Y chromosome microdeletion detection guidelines jointly published by EAA/EMQN in 2014, the detection site includes 6 basic Y chromosome-specific STS loci (sY84, sY86, sY127, sY134, sY254, sY255, AZFa, b, c each two sites) and autonomously selected extensibility STSs (sY82, sY83, sY1064, sY1065, sY1182, sY88, sY105, sY121, sY1224, sY143, sY1192, sY153, sY160). However, due to the variety and complexity of CNVs in the AZF region, especially AZFc, even the expansion sites is far from sufficient for genotyping ( Esteves et al, 2017 ; Yamaguchi et al, 2020 ; Zhang et al, 2021 ).…”

Section: Repetitive Sequences Of the Y Chromosomementioning

confidence: 99%

Repetitive DNA Sequences in the Human Y Chromosome and Male Infertility

Xu¹,

Pang

2022

Front. Cell Dev. Biol.

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The male-specific Y chromosome, which is well known for its diverse and complex repetitive sequences, has different sizes, genome structures, contents and evolutionary trajectories from other chromosomes and is of great significance for testis development and function. The large number of repetitive sequences and palindrome structure of the Y chromosome play an important role in maintaining the stability of male sex determining genes, although they can also cause non-allelic homologous recombination within the chromosome. Deletion of certain Y chromosome sequences will lead to spermatogenesis disorders and male infertility. And Y chromosome genes are also involved in the occurrence of reproductive system cancers and can increase the susceptibility of other tumors. In addition, the Y chromosome has very special value in the personal identification and parentage testing of male-related cases in forensic medicine because of its unique paternal genetic characteristics. In view of the extremely high frequency and complexity of gene rearrangements and the limitations of sequencing technology, the analysis of Y chromosome sequences and the study of Y-gene function still have many unsolved problems. This article will introduce the structure and repetitive sequence of the Y chromosome, summarize the correlation between Y chromosome various sequence deletions and male infertility for understanding the repetitive sequence of Y chromosome more systematically, in order to provide research motivation for further explore of the molecules mechanism of Y-deletion and male infertility and theoretical foundations for the transformation of basic research into applications in clinical medicine and forensic medicine.

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Poor intracytoplasmic sperm injection outcome in infertile males with azoospermia factor c microdeletions

Cited by 15 publications

References 35 publications

Clinical Outcomes and Live Birth Rate Resulted From Microdissection Testicular Sperm Extraction With ICSI-IVF in Non-Obstructive Azoospermia: A Single-Center Cohort Study

Clinical Outcomes and Live Birth Rate Resulted From Microdissection Testicular Sperm Extraction With ICSI-IVF in Non-Obstructive Azoospermia: A Single-Center Cohort Study

Does ICSI outcome in obstructive azoospermia differ according to the origin of retrieved spermatozoa or the cause of epididymal obstruction? A comparative study

Repetitive DNA Sequences in the Human Y Chromosome and Male Infertility

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