Poor correlation between the levels of proteinase inhibitors found in seeds of different cultivars of cowpea (Vigna unguiculata) and the resistance/susceptibility to predation by Callosobruchus maculatus

Xavier‐Filho, J.; Campos, Francisco; Ary, Maria B.; Silva, Carlos P.; Carvalho, Marta M. M.; Macedo, Maria Lígia Rodrigues; Lemos, Francisco José Alves; Grant, George

doi:10.1021/jf00088a071

Cited by 103 publications

(51 citation statements)

References 29 publications

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“…The tubes, in triplicate, were centrifuged (5,000 g for 10 min at 25°C) and the supernatants were alkalinised with 400 µL of 2.0 N NaOH solution. The absorbance of the sample at 420 nm was then measured (Xavier-Filho et al 1989). One unit of activity (AU) was defined as the amount of enzyme capable of changing the absorbance at 420 nm by 0.01.…”

Section: Methodsmentioning

confidence: 99%

Potential of laticifer fluids for inhibiting Aedes aegypti larval development: evidence for the involvement of proteolytic activity

Ramos¹,

Pereira²,

Souza³

et al. 2009

Mem. Inst. Oswaldo Cruz

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Section: Methodsmentioning

confidence: 99%

Potential of laticifer fluids for inhibiting Aedes aegypti larval development: evidence for the involvement of proteolytic activity

Ramos¹,

Pereira²,

Souza³

et al. 2009

Mem. Inst. Oswaldo Cruz

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“…The total proteolytic activity of L. leucochephala seed extracts was examined using azocasein as a nonspecific substrate (XAVIER-FILHO et al, 1989). One unit of activity (UA) was defined as the amount of enzyme capable of increasing absorbance by 420 nm at 0.01 mL -1 in 60 min.…”

Section: Protein Extraction and Quantificationmentioning

confidence: 99%

Anthelmintic activity of Leucaena leucocephala protein extracts on Haemonchus contortus

Soares

Araújo

Lopes

et al. 2015

Rev. Bras. Parasitol. Vet.

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The objective of this study was to evaluate the effects of protein extracts obtained from the plant Leucaena leucocephala on the nematode parasite Haemonchus contortus. The seeds, shell and cotyledon of L. leucocephala were separated and their proteins extracted using a sodium phosphate buffer, and named as TE (total seed extract), SE (shell extract) and CE (cotyledon extract). Soluble protein content, protease, protease inhibitory and chitinase activity assays were performed. Exsheathment inhibition of H. contortus larvae were performed at concentrations of 0.6 mg mL . The effective concentration for 50% hatching inhibition (EC 50 ) was estimated by probit. Different proportions of soluble proteins, protease and chitinase were found in TE and CE. Protease inhibitory activity was detected in all extracts. The EC 50 of the CE and TE extracts were 0.48 and 0.33 mg mL -1 , respectively. No ovicidal effects on H. contortus were detected in SE extracts, and none of the protein extracts demonstrated larvicidal effects on H. contortus. We therefore conclude that protein extracts of L. leucocephala had a detrimental effect on nematode eggs, which can be correlated with the high protease and chitinase activity of these extracts.Keywords: Plant proteins, protease, protease inhibitor, chitinase. ResumoO objetivo deste estudo foi avaliar a atividade anti-helmíntica de extratos proteicos de leucena (Leucaena leucocephala) sobre Haemonchus contortus. As sementes, as cascas e os cotilédones foram moídos separadamente e as proteínas extraídas com tampão fosfato de sódio e denominados: TE (extrato total), SE (extrato casca) e CE (extrato cotilédone). O teor de proteínas, atividade proteolítica, inibitória de protease e quitinolítica dos extratos foram verificados, além da ação sobre a eclosão de ovos e desembainhamento larvar de H. contortus. A concentração efetiva para inibição de 50% da eclosão dos ovos (EC 50 ) foi calculada através do probit. Foi demonstrado que TE e CE possuem, em diferentes proporções, proteínas solúveis, protease e quitinase. Atividade inibitória de protease foi encontrada em todos os extratos. A EC 50 dos extratos CE e TE foram 0,48 e 0,33 mg de proteína mL -1 , respectivamente. O extrato SE não apresentou atividade sobre a eclosão dos ovos. Os extratos proteicos não apresentaram efeito larvicida sobre H. contortus. Conclui-se que a ação de extratos proteicos de L. leucocephala afetam negativamente a eclodibilidade dos ovos, correlacionando-se com a alta atividade de protease e quitinase dos extratos testados.Palavras-chaves: Proteínas vegetais, protease, inibidor de protease, quitinase.

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“…The ability of CpaTI to inhibit other serine proteinases (bovine chymotrypsin and porcine elastase) and cysteine proteinases (papain and bromelain) was assayed using azocasein as substrate, as described by Xavier-Filho et al [28]. All assays were performed in triplicate.…”

Section: Specificity Of Cpati For Serine and Cysteine Proteinasesmentioning

confidence: 99%

Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)

Gomes

Barbosa

Macedo

et al. 2005

Plant Physiology and Biochemistry

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Poor correlation between the levels of proteinase inhibitors found in seeds of different cultivars of cowpea (Vigna unguiculata) and the resistance/susceptibility to predation by Callosobruchus maculatus

Cited by 103 publications

References 29 publications

Potential of laticifer fluids for inhibiting Aedes aegypti larval development: evidence for the involvement of proteolytic activity

Potential of laticifer fluids for inhibiting Aedes aegypti larval development: evidence for the involvement of proteolytic activity

Anthelmintic activity of Leucaena leucocephala protein extracts on Haemonchus contortus

Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)

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