Ponazuril inhibits the development of Eimeria vermiformis in experimentally infected outbred Swiss mice

Abstract

We evaluated a 15% paste formulation of ponazuril in outbred Swiss mice that were experimentally infected with Eimeria vermiformis. Thirty, 8-week-old female mice (approximately 20 g) were placed in one group of 10 mice and one group of 20 mice. Mice in both groups were gavaged with approximately 5,000 sporulated oocysts of E. vermiformis on day 0. Mice in group 2 (n=10) were treated orally on days 3 and 4 with ponazuril (suspended in 30% propylene glycol) at the rate of 20 mg/kg. Mice in group 1 (n=20) were gavaged with a similar volume of 30% propylene glycol. Rates of oocyst passage (oocysts/g feces) were determined on day 10 (peak patency) for treated and nontreated mice using a fecal aliquot oocyst counting technique. Oocysts were not observed in the feces of treated mice using the aliquot technique. Control mice passaged oocysts at a geometric mean rate of >104,000 oocysts/g feces. Control mice also produced significantly less feces on day 10. These results indicate that ponazuril is effective against E. vermiformis under the conditions utilized in this study, and that the E. vermiformis mouse model could be useful in predicting the efficacy of new anticoccidial drugs.

Detection, quantifications, and pharmacokinetics of ponazuril in healthy swine

“…Ponazuril had been evaluated for the treatment of equine protozoal myeloencephalitis (EPM) in a study sponsored by Bayer Animal Health (Pittsburgh, PA, USA; Furr & Kennedy, ). In addition to horses, ponazuril also is an effective anticoccidial drug in several host species, such as pigs (Kalthoff, ; Bach et al ., ; Mundt et al ., ), sheep (Le Sueur et al ., ), goats (Balicka‐Ramisz, ), chickens (Mathis et al ., ), mice (Billeter et al ., ), cattle (Epe et al ., ; Mundt et al ., ), dogs (Charles et al ., ), and llamas (Prado et al ., ).…”