Pomegranate (Punica granatum) phenolics ameliorate hydrogen peroxide-induced oxidative stress and cytotoxicity in human keratinocytes

Liu, Chang; Guo, Hao; DaSilva, Nicholas A.; Li, Dongli; Zhang, Kun; Wan, Yinsheng; Gao, Xing‐Hua; Chen, Hong‐Duo; Seeram, Navindra P.; Ma, Hang

doi:10.1016/j.jff.2019.02.015

Cited by 59 publications

(43 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Treatment of MSX maintained the normal shape of cell nuclei, as compared to H 2 O 2 and MGO challenged cells, which had irregular shaped nuclei (Figure 1D). This finding is in agreement with our previously reported study showing that phenolics from a commercially available standardized pomegranate fruit extract (Pomella) attenuated H 2 O 2 ‐induced cytotoxicity in HaCaT cells 21 …”

Section: Resultssupporting

confidence: 94%

“…Our laboratory has initiated a research program focused on the identification of AGE inhibitors from several medicinal plants and functional foods (foods that provide health benefits in addition to macro‐ and micronutrients) 12‐20 . Among these natural products, we reported that phenolic‐enriched extracts of pomegranate fruit ( Punica granatum ) and red maple leaves ( Acer rubrum ) showed potent inhibitory effects against AGEs formation and protected human keratinocytes against oxidative stress induced cytotoxicity ( 12,15,16,21 ). In addition, we also reported that a polyphenol‐enriched maple syrup extract (MSX) showed inhibitory activity against AGEs formation and protective effects against AGEs‐ and MGO‐induced cytotoxicity in normal human colon cells 13 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phenolic‐enriched maple syrup extract protects human keratinocytes against hydrogen peroxide and methylglyoxal induced cytotoxicity

Sheng

Liu

Petrovas

et al. 2020

Dermatologic Therapy

Self Cite

View full text Add to dashboard Cite

Reactive carbonyl species including methylglyoxal (MGO) are oxidation metabolites of glucose and precursors of advanced glycation end products (AGEs). They are important mediators of cellular oxidative stress and exacerbate skin complications. Published data supports that certain phenolic compounds can exert cellular protective effects by their antioxidant activity. A phenolic‐enriched maple syrup extract (MSX) was previously reported to show protective effects against AGEs‐ and MGO‐induced cytotoxicity in human colon cells but its skin protective effects remain unknown. The protective effects of MSX were evaluated against hydrogen peroxide (H2O2)‐ and MGO‐induced cytotoxicity in human keratinocytes (HaCaT cells). Cellular viability and antioxidant activity were evaluated by the luminescent cell viability CellTiter‐Glo assay and the reactive oxygen species (ROS) assay, respectively. A single‐cell gel electrophoresis (Comet assay) was used to measure the strand breaks in the DNA of HaCaT cells. MSX (at 50 μg/mL) ameliorated H2O2‐ and MGO‐induced cytotoxicity by increasing cell viability by 21.5% and 25.9%, respectively. MSX reduced H2O2‐ and MGO‐induced ROS production by 69.4% and 56.6%, respectively. MSX also reduced MGO‐induced DNA damage by 47.5%. MSX showed protective effects against H2O2‐ and MGO‐induced cytotoxicity in HaCaT cells supporting its potential for dermatological and/or cosmeceutical applications.

show abstract

Section: Resultssupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Phenolic‐enriched maple syrup extract protects human keratinocytes against hydrogen peroxide and methylglyoxal induced cytotoxicity

Sheng

Liu

Petrovas

et al. 2020

Dermatologic Therapy

Self Cite

View full text Add to dashboard Cite

show abstract

“…Given the promising physico-chemical characteristics of the vesicles (i.e., small size and homogeneity) and their high biocompatibility in skin cells (over 100% of fibroblast viability), further studies were carried out. In particular, the ability of the extract to protect skin cells from oxidative The exposure of fibroblasts to hydrogen peroxide increases the intracellular ROS concentration causing cell death via apoptosis or necrosis [27]. The results in Figure 4 confirmed a marked cell death after exposure to hydrogen peroxide, with an approximate 50% survival.…”

Section: Antioxidant Assaymentioning

confidence: 75%

Incorporation of Lippia citriodora Microwave Extract into Total-Green Biogelatin-Phospholipid Vesicles to Improve Its Antioxidant Activity

Leyva-Jiménez¹,

Manca

Manconi

et al. 2020

Nanomaterials

View full text Add to dashboard Cite

Phytochemicals from Lippia citriodora leaves were extracted by applying an innovative technology based on the use of microwaves, which represents an alternative method to extract bioactive substances. The obtained extract was incorporated into phospholipid vesicles in order to promote the antioxidant effect of the bioactive molecules present in L. citriodora extract. The extract was analyzed by High Performance Liquid Chromatography coupled to Time-Of-Flight mass spectrometer by electrospray (HPLC-ESI-TOF-MS) and different phytochemicals were detected and quantified. The whole extract was incorporated in liposomes, glycerosomes (liposomes modified with glycerol) and propylene glycol-containing vesicles (PG-PEVs). Moreover, a biopolymer obtained from fish by-product, that is Thunnus albacares skin, was added to improve the bioactivity of the formulations. The in vitro biocompatibility and the antioxidant efficacy of the extract in solution or loaded in the vesicles were tested in primary mouse embryonic fibroblasts (3T3). The results showed the superior bioactivity of the vesicle formulations over the aqueous solution of the extract, which points to an interesting strategy for the treatment of skin disorders.

show abstract

“…whole fruit extract ↓H 2 O 2 -induced oxidative stress; ↓apoptosis; natural antioxidants for skin health [37] whole fruit extract antimicrobial activity against 29 clinical Clostridium difficile isolates [38] juice extract inhibition of a-glucosidase activity [39] extract and juice prebiotic effect [40] juice ↓lipogenesis and lipolysis [41] juice inhibition of lipase, α-glucosidase and dipeptidyl peptidase-4 [42] peel extract inhibition of renal cell carcinoma growth [43] peel extract anti-neurodegenerative [44] peel extract ↑apoptosis and ↓metastasis in prostate cancer cells [45] peel and juice extract inhibition of cariogenic bacteria [46,47] peel and fruit extract stimulates osteoblastic differentiation (osteoporosis) [48,49] peel polysaccharide immunostimulatory effect [50] punicalagin antiproliferative activity against human lung, breast, cervical and prostate cancer cells [51][52][53] punicalagin ↑papillary thyroid human carcinoma cell death [54] punicalagin inhibition of lipopolysaccharide-induced memory impairment (Alzheimer's disease) [55] punicalagin attenuates osteoclast differentiation (osteoporosis) [56] pomegranate-derived products (juice, extract, oil) photo-chemopreventive effect in human reconstituted skin [57] urolithins inhibition of neuroinflammation (Alzheimer's disease) [58] 3.1.…”

Section: Derivative Effect Referencesmentioning

confidence: 99%

Food Applications and Potential Health Benefits of Pomegranate and its Derivatives

Kandylis

Kokkinomagoulos

2020

Foods

185

112

View full text Add to dashboard Cite

Pomegranate (Punica granatum L.) is an ancient fruit that is particularly cultivated in west Asia, though it is also cultivated in the Mediterranean region and other parts of the world. Since ancient years, its consumption has been associated with numerous health benefits. In recent years, several in vitro and in vivo studies have revealed its beneficial physiological activities, especially its antioxidative, antimicrobial and anti-inflammatory properties. Furthermore, human-based studies have shown promising results and have indicated pomegranate potential as a protective agent of several diseases. Following that trend and the food industry’s demand for antioxidants and antimicrobials from natural sources, the application of pomegranate and its extracts (mainly as antioxidants and antimicrobials), has been studied extensively in different types of food products with satisfactory results. This review aims to present all the recent studies and trends in the applications of pomegranate in the food industry and how these trends have affected product’s physicochemical characteristics and shelf-life. In addition, recent in vitro and in vivo studies are presented in order to reveal pomegranate’s potential in the treatment of several diseases.

show abstract

Pomegranate (Punica granatum) phenolics ameliorate hydrogen peroxide-induced oxidative stress and cytotoxicity in human keratinocytes

Cited by 59 publications

References 45 publications

Phenolic‐enriched maple syrup extract protects human keratinocytes against hydrogen peroxide and methylglyoxal induced cytotoxicity

Phenolic‐enriched maple syrup extract protects human keratinocytes against hydrogen peroxide and methylglyoxal induced cytotoxicity

Incorporation of Lippia citriodora Microwave Extract into Total-Green Biogelatin-Phospholipid Vesicles to Improve Its Antioxidant Activity

Food Applications and Potential Health Benefits of Pomegranate and its Derivatives

Contact Info

Product

Resources

About