Pomegranate morpho-chemodiversity: computational investigations based on in-vivo and in-vitro screening

Ejjilani, Assia; Houmanat, Karim; Hanine, Hafida; Hssaini, Lahcen; Elfazazi, Kaoutar; Hernández, Francisca; Hmid, Ilham; Razouk, Rachid

doi:10.1016/j.heliyon.2022.e09345

Cited by 3 publications

(6 citation statements)

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“…The six fully ripe fruits were randomly collected from each genotype tree, The ripeness degree was detected visually when fruits lost all traces of green color and became tinged with a yellowish or reddish color, depending on the genotypes. After that, morphological traits, such as the weight (gm) per fruit, with a weight of 100 Arils (gm) were recorded using an accuracy balance, while the thickness of the pomegranate peel (mm) was determined using a digital caliper (Ejjilani et al 2022).…”

Section: Morphological Parameters Studiedmentioning

confidence: 99%

“…Primers 17, NLSSR3, and 16, which showed the highest polymorphism percentages of 90.41, 80.00, and 76.47%, respectively, with the highest number of unique bands of 6, 6, and 9, respectively. Genotype C ampli ed the highest number of DNA fragments (115) and unique bands (13). (PIC) ranged from 0.449 to 0.768, while genetic diversity ranged from 0.56 to 0.80.…”

Section: Issr Markers With Polymorphism Parametersmentioning

confidence: 99%

“…In addition, pomegranate accessions for economic and industrial purposes were greatly necessary and selected depending on the development of agronomic and biochemical potential, databases, and genetic programs (Ejjilani et al 2022). Thus, many researchers have focused mostly on local cultivars for morphological fruit properties such as fruit fresh weight, skin thickness, and physio-chemical descriptors (Hmid et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, many researchers have focused mostly on local cultivars for morphological fruit properties such as fruit fresh weight, skin thickness, and physio-chemical descriptors (Hmid et al 2018). Morphometric and biochemical characters can enhance the signi cance of the discernment and classi cation of local germplasm such that it could be the basis for further use and also contribute to the improvement of industrial processes using pomegranate as a raw material (Ejjilani et al 2022). On the other side, local cultivars could also provide a crucial source of genes for breeding, especially in pedoclimatic conditions (Karapetsi et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…All of the above aspects are guiding pillars for the enhancement and development of the species and contribute to its competitiveness globally. Therefore, to obtain novel pomegranate cultivars with higher yield and better quality, it is essential to screen available pomegranate genetic resources and conserve them for better orienting breeding programs (Ejjilani et al 2022).…”

Section: Introductionmentioning

confidence: 99%

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Morpho-phytochemical Screening and Molecular Diversity of Pomegranate Accessions Grown in Halabja Governorate, Kurdistan Region-Iraq

Faraj

2023

Preprint

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Halabja governorate in the Kurdistan region-Iraq is famous for having high-quality pomegranate accessions. The current study was conducted to evaluate the morpho-phytochemical amount of pomegranate functional food and the genetic diversity as an important tool for the characterization of the genetic resources for germplasm management and the identification of the best genotypes for genetic improvement. In this study, phenotypical parameter, highly significant morphometric differences (P ≤ 0.05) among 24 genotypes, with the mean values of fruit weight, peel thickness, and weight of 100 Arils, total flavonoid content (TFC) and total phenolic content (TPC), antioxidant activity, total soluble solid content (TSS) 299.21 g, 3.47 mm and 38.59 g, 54.50,47.97 µg/ml, 21.08 µg/ml and 14.59 Brix respectively. In addition, inter-simple sequence repeat (ISSR) was utilized to assess the genetic diversity of the collected pomegranate genotypes. Twelve random primers of produced products plus a number of the amplified primer bands ranging from 3 to 12 and total number of 83 amplified bands were produced, among which 78 bands were polymorphic and 5 bands were monomorphic. The highest, lowest, and mean values of polymorphic bands were (11, 3, and 6.5), respectively. The PIC values ranged from 0.58 to 0.90. The dendrogram clusters for all the selections showed dissimilarity coefficients ranging between 0.22 to 0.23 (G4 vs. G5) to 0.63 (G13 vs. G14), and five groups (A, B, C, D, and F) with a mean dissimilarity (0.49). According to the results, morphometric and biochemical properties are significant aspects of development, discernment, and classification. Alongside with the ISSR marker, which is a useful technique for allowing the identification of different selections and assessing the genetic similarity among studied pomegranate accessions, this would facilitate their use as identified genetic stock in future breeding programs.

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Section: Morphological Parameters Studiedmentioning

confidence: 99%

Section: Issr Markers With Polymorphism Parametersmentioning

confidence: 99%