Polyurethane membrane/knitted mesh-reinforced collagen–chitosan bilayer dermal substitute for the repair of full-thickness skin defects via a two-step procedure

Wang, Xingang; Wu, Pan; Hu, Xiuyuan; You, Chuangang; Guo, Rui; Shi, Hua; Guo, Songxue; Zhou, Hanlei; Yu, Chun-Hong; Zhang, Yuanhai; Han, Chunmao

doi:10.1016/j.jmbbm.2015.11.021

Cited by 55 publications

(40 citation statements)

References 48 publications

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“…Treatment of burn wounds is difficult and clinical outcomes are often poor due to additional complications like scarring and disability in movement . The treatment of burn wounds has largely been centered on the development of autografts or artificial skin constructs . The standard‐of‐care for third‐degree burn wounds is the application of autologous split‐thickness skin grafts (STSGs).…”

Section: Introductionmentioning

confidence: 99%

In Situ Forming Injectable Silk Fibroin Hydrogel Promotes Skin Regeneration in Full Thickness Burn Wounds

Chouhan

Lohe

Samudrala

2018

Adv Healthcare Materials

176

128

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Full‐thickness skin wounds, associated with deep burns or chronic wounds pose a major clinical problem. Herein, the development of in situ forming hydrogel using a natural silk fibroin (SF) biomaterial for treating burn wounds is reported. Blends of SF solutions isolated from Bombyx mori and Antheraea assama show inherent self‐assembly between silk proteins and lead to irreversible gelation at body temperature. Investigation of the gelation mechanism reveals crosslinking due to formation of β‐sheet structures as examined by X‐ray diffraction and Fourier transform infrared spectroscopy. The SF hydrogel supports proliferation of primary human dermal fibroblasts and migration of keratinocytes comparable to collagen gel (Col) as examined under in vitro conditions. The SF hydrogel also provides an instructive and supportive matrix to the full‐thickness third‐degree burn wounds in vivo. A 3‐week comparative study with Col indicates that SF hydrogel not only promotes wound healing but also shows transitions from inflammation to proliferation stage as observed through the expression of TNF‐α and CD163 genes. Further, deposition and remodeling of collagen type I and III fibers suggests an enhanced overall tissue regeneration. Comparable results with Col demonstrate the SF hydrogel as an effective and inexpensive formulation toward a potential therapeutic approach for burn wound treatment.

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Section: Introductionmentioning

confidence: 99%

In Situ Forming Injectable Silk Fibroin Hydrogel Promotes Skin Regeneration in Full Thickness Burn Wounds

Chouhan

Lohe

Samudrala

2018

Adv Healthcare Materials

176

128

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“…Moreover, 19.7% of the studies evaluated CS in the regeneration of skin [24, 27, 29, 63–71], 14.7% in nervous tissue [30, 72–79], 11.5% in cartilage [32, 81–84, 87, 88], and 3.3% in periodontal [33, 90] structures. The remaining studies involved regeneration of colorectal [26], mammary [89], tympanic membrane [31], and vascular [34] tissues (1.6% each).…”

Section: Resultsmentioning

confidence: 99%

“…Only 6.6% of the studies [27, 41, 71, 85] showed no evidence of the scaffolds having a positive influence on tissue regeneration.…”

Section: Resultsmentioning

confidence: 99%

“…In fact, some studies have clearly shown that the association of CS with collagen [42, 44, 68], silk fibroin [73], and HA [45, 57, 58] was capable of promoting greater tissue regeneration than pure CS, as in these studies pure or combined CS scaffolds were implanted, making it possible to establish a comparison between them. However, in the vast majority of studies evaluating CS combined with other biomaterials, the favorable results obtained cannot be attributed to the associations, as pure CS was neither evaluated nor used as a control [8, 30, 32, 41, 46, 48, 49, 53, 54, 62, 63, 67, 71, 76, 77, 80, 81, 83, 86, 88]. …”

Section: Discussionmentioning

confidence: 99%

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Versatility of Chitosan-Based Biomaterials and Their Use as Scaffolds for Tissue Regeneration

Ribeiro

Vieira

Melo

et al. 2017

The Scientific World Journal

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Chitosan is a naturally occurring polysaccharide obtained from chitin, present in abundance in the exoskeletons of crustaceans and insects. It has aroused great interest as a biomaterial for tissue engineering on account of its biocompatibility and biodegradation and its affinity for biomolecules. A significant number of research groups have investigated the application of chitosan as scaffolds for tissue regeneration. However, there is a wide variability in terms of physicochemical characteristics of chitosan used in some studies and its combinations with other biomaterials, making it difficult to compare results and standardize its properties. The current systematic review of literature on the use of chitosan for tissue regeneration consisted of a study of 478 articles in the PubMed database, which resulted, after applying inclusion criteria, in the selection of 61 catalogued, critically analysed works. The results demonstrated the effectiveness of chitosan-based biomaterials in 93.4% of the studies reviewed, whether or not combined with cells and growth factors, in the regeneration of various types of tissues in animals. However, the absence of clinical studies in humans, the inadequate experimental designs, and the lack of information concerning chitosan's characteristics limit the reproducibility and relevance of studies and the clinical applicability of chitosan.

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“…Collagen is a natural protein and the major component of the extracellular matrix (ECM); moreover, it has an important structural and mechanical role in skin. It is known as an active role player in cell adhesion and proliferation, therefore, it is a widely used natural biopolymer in skin tissue engineering . Nonetheless, collagen is mechanically weak.…”

Section: Introductionmentioning

confidence: 99%

Collagen‐coated nano‐electrospun PCL seeded with human endometrial stem cells for skin tissue engineering applications

Sharif

Azami

et al. 2017

J Biomed Mater Res

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Human endometrial stem cells (hEnSCs) are known as an attractive source of stem cells for regenerative medicine. hEnSCs are easily isolated and are capable of repairing uterine through their strong ability of creating new capillaries. In this study, a three-dimensional (3D) nanofibrous polycaprolactone (PCL)/collagen scaffold was fabricated and characterized in order to be applied as a new approach for skin reconstruction. Furthermore, the behavior of hEnSCs on this scaffold was investigated. First, a PCL 3D scaffold was constructed using electrospinning technique. Plasma treated and PCL was grafted by collagen. The constructs were characterized for mechanical and structural properties. Cell attachment, proliferation, viability, and differentiation of hEnSCs were assessed after being seeded on PCL and PCL/collagen scaffolds using scanning electron microscopy, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, and real-time polymerase chain reaction tests. The results showed higher wettability for the PCL/collagen scaffold with desirable mechanical and structural characteristics compared to PCL and collagen alone. The attachment and proliferation rates of hEnSCs on the PCL/collagen scaffold were higher compared to those on the bare PCL. Hence, hEnSCs are newly discovered stem cell source for skin tissue engineering in vitro, particularly when developed on PCL/collagen nanofiber scaffolds. Therefore, application of hEnSCs for skin regeneration is a novel therapeutic approach for temporary skin substitute. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1578-1586, 2018.

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Polyurethane membrane/knitted mesh-reinforced collagen–chitosan bilayer dermal substitute for the repair of full-thickness skin defects via a two-step procedure

Cited by 55 publications

References 48 publications

In Situ Forming Injectable Silk Fibroin Hydrogel Promotes Skin Regeneration in Full Thickness Burn Wounds

In Situ Forming Injectable Silk Fibroin Hydrogel Promotes Skin Regeneration in Full Thickness Burn Wounds

Versatility of Chitosan-Based Biomaterials and Their Use as Scaffolds for Tissue Regeneration

Collagen‐coated nano‐electrospun PCL seeded with human endometrial stem cells for skin tissue engineering applications

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