Polyurethane foam scaffold as in vitro model for breast cancer bone metastasis

Angeloni, Valentina; Contessi, Nicola; Marco, Cinzia De; Bertoldi, Serena; Tanzi, Maria Cristina; Daidone, Maria Grazia; Farè, Silvia

doi:10.1016/j.actbio.2017.09.017

Cited by 59 publications

(32 citation statements)

References 56 publications

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“…This is likely because cells cultured on 2D systems have unlimited access to the ingredients of the medium such as oxygen, nutrients, metabolites, and signal molecules, thus resulting in higher proliferation rates . Additionally, the 2D substrate assisted in cell spreading and its mechanical stiffness positively affected cell growth . No significant difference was detected among the various 3D culture conditions for at least 7 d. At a late stage, scaffolds that were mechanically stretched (GA yarns MS) showed significantly higher proliferation profiles.…”

Section: Resultsmentioning

confidence: 99%

Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell‐Laden Hydrogel Yarns

Rinoldi

Costantini

Kijeńska‐Gawrońska

et al. 2019

Adv Healthcare Materials

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Fiber‐based approaches hold great promise for tendon tissue engineering enabling the possibility of manufacturing aligned hydrogel filaments that can guide collagen fiber orientation, thereby providing a biomimetic micro‐environment for cell attachment, orientation, migration, and proliferation. In this study, a 3D system composed of cell‐laden, highly aligned hydrogel yarns is designed and obtained via wet spinning in order to reproduce the morphology and structure of tendon fascicles. A bioink composed of alginate and gelatin methacryloyl (GelMA) is optimized for spinning and loaded with human bone morrow mesenchymal stem cells (hBM‐MSCs). The produced scaffolds are subjected to mechanical stretching to recapitulate the strains occurring in native tendon tissue. Stem cell differentiation is promoted by addition of bone morphogenetic protein 12 (BMP‐12) in the culture medium. The aligned orientation of the fibers combined with mechanical stimulation results in highly preferential longitudinal cell orientation and demonstrates enhanced collagen type I and III expression. Additionally, the combination of biochemical and mechanical stimulations promotes the expression of specific tenogenic markers, signatures of efficient cell differentiation towards tendon. The obtained results suggest that the proposed 3D cell‐laden aligned system can be used for engineering of scaffolds for tendon regeneration.

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Section: Resultsmentioning

confidence: 99%

Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell‐Laden Hydrogel Yarns

Rinoldi

Costantini

Kijeńska‐Gawrońska

et al. 2019

Adv Healthcare Materials

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“…2 More recently, 3D in vitro models have emerged and are being developed as low cost, promising alternatives to animals. 7,[29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48] However, most studies focus on the development of the actual 3D model and, to the best of our knowledge, there are very limited 3D studies on PDAC treatment screening (chemotherapy and radiotherapy) with most studies conducted in 3D spheroids (cell aggregates). 14,32,[34][35][36][49][50][51][52] Generally, these studies report a higher resistance of PDAC to treatment in 3D when compared to 2D cultures, a trend which is in closer alignment with in vivo studies.…”

Section: Introductionmentioning

confidence: 99%

Chemoradiotherapy screening in a novel biomimetic polymer based pancreatic cancer model

et al. 2019

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“…In the recent years, the Authors' research group acquired the know-how for the preparation of biocompatible polyurethane foams prepared by a one-step expansion procedure using water as expanding agent [22][23][24]. Among other applications, the developed 3D PU scaffolds were investigated for bone tissue engineering [25] and as in vitro model for breast cancer bone metastasis [26]. However, by an appropriate selection of the regents and relative stoichiometry, PU scaffold could be designed for regeneration of soft tissues, such as skin and adipose tissue.…”

Section: Methodsmentioning

confidence: 99%

Polymers and Scaffolds with Improved Blood Compatibility and Enhanced Cellular Response with Focus on Polyurethane Foams Functionalized with Amino-Amide Groups

Tanzi¹,

Gantz²,

Bertoldi³

et al. 2019

J. Adv. Biotechnol. Bioeng.

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Biofunctionalization of biomaterial substrates has gained increasing interest in Tissue Engineering (TE) to create functional scaffolds with improved performances and capable of directing cellular behaviour. This work takes into account the functionalization of polyurethane (PU) foams with an active molecule, the diamino-diamide diol PIME, purposely designed to be inserted in the structure of linear PUs and previously found to be able to improve hemo-, cytocompatibility and bacterial resistance of these biomaterials. Here, for the first time, the gas foaming process previously set up for biocompatible PU foams was modified, and different methods were tested to obtain structurally stable foams containing PIME. The obtained foams and respective controls showed appropriate porosity (Ø = 407 ÷ 589 ± 30 µm; > 90% open porosity), similar density (≈ 0.14 g/cm 3) and hydrophilicity (water uptake ≈ 300%) and adequate compressive properties (E = 0.058 ÷ 0.170 Mpa; Hysteresis Area = 2.07 ÷ 5.59 x 10-3 J/cm 3 , < 5% residual deformation). PU foams containing PIME exhibited a more spherical pore geometry; the foam obtained by applying a higher stirring speed during synthesis (PU-3kP) exhibited a lower mean pore size and an elastic modulus higher than that of the other foams. FT-IR spectroscopy indicated the presence of new absorption bands and structural changes attributable to the presence of PIME. Cytotoxicity tests performed with the cell line L929 verified the absence of cytotoxic effects. All foams were able to support L929 fibroblasts proliferation and vitality for seven days; PIME-containing foams showed higher values of cell proliferation (p<0.05) that the respective controls.

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Polyurethane foam scaffold as in vitro model for breast cancer bone metastasis

Cited by 59 publications

References 56 publications

Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell‐Laden Hydrogel Yarns

Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell‐Laden Hydrogel Yarns

Chemoradiotherapy screening in a novel biomimetic polymer based pancreatic cancer model

Polymers and Scaffolds with Improved Blood Compatibility and Enhanced Cellular Response with Focus on Polyurethane Foams Functionalized with Amino-Amide Groups

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