Polyunsaturated fatty acids in the modulation of T-cell signalling

Khan, Naim Akhtar

doi:10.1016/j.plefa.2010.02.023

Cited by 33 publications

(15 citation statements)

References 119 publications

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“…Several epidemiologic and clinical studies have shown that n-3 PUFAs are able to provide beneficial effects in a wide variety of pathologies ranging from autoimmune and inflammatory diseases to neurological and psychiatric disorders and, in particular, to several types of malignancy, including ovarian, pancreatic, prostate, renal, colorectal and breast cancer [31-33]. …”

Section: Discussionmentioning

confidence: 99%

Effects of n-3 PUFAs on breast cancer cells through their incorporation in plasma membrane

et al. 2011

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BackgroundPUFAs are important molecules for membrane order and function; they can modify inflammation-inducible cytokines production, eicosanoid production, plasma triacylglycerol synthesis and gene expression. Recent studies suggest that n-3 PUFAs can be cancer chemopreventive, chemosuppressive and auxiliary agents for cancer therapy. N-3 PUFAs could alter cancer growth influencing cell replication, cell cycle, and cell death. The question that remains to be answered is how n-3 PUFAs can affect so many physiological processes. We hypothesize that n-3 PUFAs alter membrane stability, modifying cellular signalling in breast cancer cells.MethodsTwo lines of human breast cancer cells characterized by different expression of ER and EGFR receptors were treated with AA, EPA or DHA. We have used the MTT viability test and expression of apoptotic markers to evaluate the effect of PUFAs on cancer growth. Phospholipids were analysed by HPLC/GC, to assess n-3 incorporation into the cell membrane.ResultsWe have observed that EPA and DHA induce cell apoptosis, a reduction of cell viability and the expression of Bcl2 and procaspase-8. Moreover, DHA slightly reduces the concentration of EGFR but EPA has no effect. Both EPA and DHA reduce the activation of EGFR.N-3 fatty acids are partially metabolized in both cell lines; AA is integrated without being further metabolized. We have analysed the fatty acid pattern in membrane phospholipids where they are incorporated with different degrees of specificity. N-3 PUFAs influence the n-6 content and vice versa.ConclusionsOur results indicate that n-3 PUFA feeding might induce modifications of breast cancer membrane structure that increases the degree of fatty acid unsaturation. This paper underlines the importance of nutritional factors on health maintenance and on disease prevention.

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Section: Discussionmentioning

confidence: 99%

Effects of n-3 PUFAs on breast cancer cells through their incorporation in plasma membrane

et al. 2011

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“…In fact, the best immunosuppressors might interfere, principally, with abnormal T-cell activation, as seen in the autoimmune diseases, without influencing the same in healthy situations [41]. As far as the mechanism of action of fatty acids is concerned, it has been well established that they interfere with cell signalling, particularly with the cascade of MAP kinases like ERK1/2 and p38 [42,43]. In fact, Chan et al [18] have also demonstrated that a mixture of herbs containing Zizyphus extracts also interfere with the phosphorylation of ERK1/2 and p38 in T-cells.…”

Section: Discussionmentioning

confidence: 99%

Zizyphus lotus L. (Desf.) modulates antioxidant activity and human T-cell proliferation

Benammar

Hichami

Yessoufou

et al. 2010

BMC Complement Altern Med

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BackgroundZizyphus lotus L. (Desf.) also known as Jujube, is a deciduous shrub which belongs to Rhamnaceae family. This plant is used in Algerian traditional medicine for its anti-diabetic, sedative, analgesic, anti-inflammatory and hypoglycaemic activities. In the present study, we determined the concentrations of different vitamins (vitamin A, C and E) and fatty acids in root, stem, leaves, fruit pulp and seed of Zizyphus lotus L. (Desf.) and assessed the effects of their aqueous extracts on antioxidant status and human T-cell proliferation.MethodsAqueous filtrates from different parts, i.e, root, leaf, stem, fruit pulp and seed, of Zizyphus lotus L. (Desf.) were prepared. Vitamin C levels were determined by precipitating with 10% trichloroacetic acid and vitamin A and E were assessed by HPLC. Lipid composition of these extracts was determined by gas-liquid chromatography. Anti-oxidant capacity was evaluated by using anti-radical resistance kit [Kit Radicaux Libres (KRL@; Kirial International SA, Couternon, France)]. T-cell blastogenesis was assessed by the incorporation of 3H-thymidine. IL-2 gene expression was evaluated by RT-qPCR.ResultsOur results show that fruit pulp contained higher vitamin A and C contents than other parts of the plant. Furthermore, the fruit pulp was the richest source of linoleic acid (18:2n-6), a precursor of n-6 fatty acids. Fruit seeds possessed higher vitamin C levels than leaves, roots and stem. The leaves were the richest source of vitamin E and linolenic acid (18:3n-3), a precursor of n-3 fatty acids. The antioxidant capacity of the different extracts, measured by KRL@ test, was as follows: pulp < seed

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“…Owing to the proposed competitive role of ω3 and ω6 PUFA, their dietary composition has been suggested to be a biologically plausible target for CRC management. ω3 and ω6 FAs represent naturally occurring substances required for biological process with key roles in phospholipid membrane structure and function, cellular signaling, and lipid metabolism (11, 12). In humans, the ω3 α-linolenic acid (ALA, 18:3ω3) as well as the ω6 linoleic acid (LA, 18:2ω6), precursors of several PUFA, are essential FA that cannot be synthesized by humans and must therefore be assumed from dietary sources including fish, flaxseeds, walnuts, and algae (9).…”

Section: Dietary Polyunsaturated Fatty Acidsmentioning

confidence: 99%

ω3 Polyunsaturated Fatty Acids as Immunomodulators in Colorectal Cancer: New Potential Role in Adjuvant Therapies

Miccadei¹,

Masella

Mileo³

et al. 2016

Front. Immunol.

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Diet composition may affect the onset and progression of chronic degenerative diseases, including cancer, whose pathogenesis relies on inflammatory processes. Growing evidence indicates that diet and its components critically contribute to human health, affecting the immune system, secretion of adipokines, and metabolic pathways. Colorectal cancer (CRC) is one of the leading causes of death worldwide. Antineoplastic drugs are widely used for CRC treatment, but drug resistance and/or off-target toxicity limit their efficacy. Dietary ω3 polyunsaturated fatty acids (PUFA) have been gaining great interest in recent years as possible anti-inflammatory and anticancer agents, especially in areas such as the large bowel, where the pro-inflammatory context promotes virtually all steps of colon carcinogenesis. Growing epidemiological, experimental, and clinical evidence suggests that ω3 PUFA may play a role in several stages of CRC management exhibiting antineoplastic activity against human CRC cells, improving the efficacy of radiation and chemotherapy, ameliorating cancer-associated secondary complications, and preventing CRC recurrence. These effects are most likely related to the immunomodulatory activities of ω3 PUFA that are able to influence several aspects of the inflammatory process ranging from inflammasome activation, leukocyte recruitment, production of immune mediators to differentiation, and activation of immune cells. In this review, we will focus on the potential use of ω3 PUFA as adjuvant agents together with chemo/radiotherapy, highlighting the immunomodulatory effects most likely responsible for their beneficial effects in different stages of CRC management.

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Polyunsaturated fatty acids in the modulation of T-cell signalling

Cited by 33 publications

References 119 publications

Effects of n-3 PUFAs on breast cancer cells through their incorporation in plasma membrane

Effects of n-3 PUFAs on breast cancer cells through their incorporation in plasma membrane

Zizyphus lotus L. (Desf.) modulates antioxidant activity and human T-cell proliferation

ω3 Polyunsaturated Fatty Acids as Immunomodulators in Colorectal Cancer: New Potential Role in Adjuvant Therapies

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