Polytrauma induces increased expression of pyruvate kinase in neutrophils

Abstract

Polytrauma (PT) leads to systemic activation of polymorphonuclear neutrophils (PMNs). Organ damage commonly found in these patients is ascribed to respiratory bursts of activated PMNs. With the use of sodium dodecyl sulfate–polyacrylamide gel electrophoresis, PMN extracts from PT patients were found to contain a clear protein band not seen in control PMNs from healthy volunteers. This band was identified by amino acid sequencing and Western blotting as pyruvate kinase (PK). Enzymatic assays revealed a 600-fold increase in PK activity in PMNs of PT patients, with the highest levels occurring between the fifth and seventh posttraumatic day. In lymphocytes, no such increase was detectable. As PK is a major regulatory enzyme in glycolysis, glucose-dependent lactate production in PMNs from PT patients was assayed. These cells showed a higher glycolytic lactate production than controls. It was additionally demonstrated that acute activation of respiratory burst activity depends mainly on breakdown of glucose to lactate via the pentose-phosphate pathway and glycolysis. In PMNs from PT patients, this glucose-dependent respiratory burst activity was more than twofold higher than in controls. The increase in expression and activity of PK in PMNs from PT patients may contribute to the high glucose-dependent respiratory burst activity seen in these cells.

Transcriptional reprogramming of metabolic pathways in critically ill patients

“…We addressed this issue in a post hoc analysis; this analysis did not find any correlation between neutrophils counts and glycolytic genes (Additional file 3 ). Such a finding is in keeping with a previous study showing that an increase in glycolytic enzyme activity was independent of neutrophil counts [ 22 ]. Nevertheless, we could not exclude the effect of cell count on gene-expression (such effect, if present, was likely to be very small).…”

A Model of the Oscillatory Metabolism of Activated Neutrophils

“…Further analysis of the glycolytic pathway in neutrophils should provide further insights into the coupling of these two oscillators. Importantly, combined modeling/experimental studies should help explain cellmediated damage due to high glucose levels in diabetes and the unusual metabolic changes in neutrophils during polytrauma (Oehler et al, 2000).…”

Unusual malignant neoplasms occurring around dental implants: A report of 2 cases

“… 11 Thus, dental implants could be regarded as a form of mandibular trauma that produces ongoing inflammation, leading to the overstimulation of plasma cells, which could culminate in a malignant clone, as seen in chronic infectious disease processes. 12 13 …”