2011
DOI: 10.1089/hum.2010.179
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Polyswitch Lentivectors: “All-in-One” Lentiviral Vectors for Drug-Inducible Gene Expression, Live Selection, and Recombination Cloning

Abstract: Lentiviral vectors are now widely considered one of the safest and most efficient tools for gene delivery and stable gene expression. Even though inducible gene expression cassettes are mandatory for many genetic engineering strategies, most current systems suffer from various issues, such as the requirement of two vectors, which decreases the overall efficiency of the transduction, leakiness and/or insufficient levels of activation of the inducible promoter, lack of selectable marker, low titers, or general i… Show more

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Cited by 25 publications
(19 citation statements)
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“…Stable populations of NMuMG cells expressing the blasticidine-resistant marker together with TFAP2A-FLAG under a doxycycline-inducible promoter were obtained with the pCLX expression system [26]. Lentiviral particles were produced in HEK293-LV cells using the helper vectors pMDL, pREV and the envelope-encoding vector pVSV.…”
Section: Methodsmentioning
confidence: 99%
“…Stable populations of NMuMG cells expressing the blasticidine-resistant marker together with TFAP2A-FLAG under a doxycycline-inducible promoter were obtained with the pCLX expression system [26]. Lentiviral particles were produced in HEK293-LV cells using the helper vectors pMDL, pREV and the envelope-encoding vector pVSV.…”
Section: Methodsmentioning
confidence: 99%
“…Lentiviral vector stocks were generated using transient transfection of HEK 293T/17 cells with the specific lentivector transfer plasmid, the psPAX2 plasmid encoding gag/pol and the pCAG-VSVG envelope plasmid, as previously described. 42,43 Lentivector titer was performed using transduction of HT-1080 cells followed by flow cytometry quantification of GFP-positive (or mCherry positive) cells 5 days after infection, as previously described. 42,43 …”
Section: Methodsmentioning
confidence: 99%
“…42,43 Lentivector titer was performed using transduction of HT-1080 cells followed by flow cytometry quantification of GFP-positive (or mCherry positive) cells 5 days after infection, as previously described. 42,43 …”
Section: Methodsmentioning
confidence: 99%
“…The different expression constructs were generated using the Gateway recombination cloning technology on lentiviral destination vectors (Giry-Laterriè re et al, 2011a, 2011b. In a first type of vector, a single expression unit is composed of one promoter driving one gene.…”
Section: Plasmidsmentioning
confidence: 99%