Polystyrene microplastics (PS-MPs) toxicity induced oxidative stress and intestinal injury in nematode Caenorhabditis elegans

Yu, Yunjiang; Chen, Haibo; Hua, Xin; Dang, Yao; Han, Yuge; Yu, Zhiming; Chen, Xichao; Ding, Ping; Li, Hui

doi:10.1016/j.scitotenv.2020.138679

Cited by 124 publications

(35 citation statements)

References 52 publications

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“…Even though not addressed here, macroorganisms also contribute to soil aggregation 44 , and may be affected by microplastics in soil. It has been observed that polyethylene (PE) and polystyrene (PS) particles can be ingested by worms 17,18 and nematodes 45 , which might affect its growth rates and caused histopathological damage, that ultimately may affected soil aggregation dynamics.…”

Section: Different Microplastic Shapes Decreased Soil Aggregationmentioning

confidence: 99%

Microplastic shape, concentration and polymer type affect soil properties and plant biomass

Lozano

Lehnert

Linck

et al. 2020

Preprint

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Microplastics are an increasing concern in terrestrial systems. These particles can be incorporated into the soil in a wide range of shapes and polymers, reflecting the fact that manufacturers produce plastics in a variety of physical and chemical properties matching their intended use.Despite of this, little is known about the effects that the addition into the soil of microplastics of different shapes, polymer type and concentration levels may have on soil properties and plant performance.To fill this gap, we selected four microplastic shapes: fibers, films, foams and fragments; and for each shape we selected three microplastics made of one of the following polymers: polyester, polyamide, polypropylene, polyethylene, polyethylenterephthalat, polyurethane, polystyrene and polycarbonate. In a glasshouse experiment, each microplastic was added to a soil from a dry grassland at a concentration of 0.1%, 0.2%, 0.3% and 0,4%. A carrot (Daucus carota) plant grew in each pot during four weeks. At harvest, shoot and root mass, soil aggregation and microbial activity were measured.Our results showed that all microplastic shapes increased shoot and root masses. As concentration increased, microfibers increased plant biomass probably as fibers may hold water in the soil for longer. In contrast, microfilms decreased biomass with concentration, likely because they can create channels in the soil that promote water evaporation affecting plant performance. All microplastic shapes decreased soil aggregation, probably since microplastics may introduce fracture points in the aggregates affecting their stability and also due to potential negative effects on soil biota. The latter may also explain the decrease in microbial activity with, for example, polyethylene films. Similar to plant biomass, microfilms decreased soil aggregation with increasing concentration.Our study tested the microplastic shape mediation and dissimilarity hypotheses, highlighting the importance of microplastic shape, polymer type and concentration when studying the effects of microplastics on terrestrial systems.

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Section: Different Microplastic Shapes Decreased Soil Aggregationmentioning

confidence: 99%

Microplastic shape, concentration and polymer type affect soil properties and plant biomass

Lozano

Lehnert

Linck

et al. 2020

Preprint

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“…The reason behind this observation is still unknown to us and needs further investigation. Although some researchers have reported a negative effect of microplastics on C. elegans length 10,11,22 , others have shown similar results in terms of the non-correlating effect of microplastics on the worms' length. 24 Lastly, Figure 5F shows the length reduction of worms under various microplastic exposure conditions during anodal stimulations (electrical egg-laying) in our device.…”

Section: Effect Of Microplastics On the Electric Egg-laying Of C Elementioning

confidence: 87%

“…[19][20][21] Ingestion and intestinal accumulation of microplastics smaller than 5µm have been shown in C. elegans. 10,22,23 Microplastics negatively affected C. elegans phenotypical behaviours such as locomotion and body bend frequency as well as its growth and reproduction. [9][10][11][12][22][23][24] Lei et al 11 studied the effects of exposing C. elegans to 1 mg/L of 0.1-5μm polystyrene microparticles, using locomotory behaviours, growth, and lifespan as toxicity indicators.…”

Section: Introductionmentioning

confidence: 99%

“…10,22,23 Microplastics negatively affected C. elegans phenotypical behaviours such as locomotion and body bend frequency as well as its growth and reproduction. [9][10][11][12][22][23][24] Lei et al 11 studied the effects of exposing C. elegans to 1 mg/L of 0.1-5μm polystyrene microparticles, using locomotory behaviours, growth, and lifespan as toxicity indicators. Their investigations demonstrated that 1μm particles significantly deteriorated the survival and growth rate and caused oxidative damage to cholinergic and GABAnergic neurons, which was ameliorated by natural antioxidants such as curcumin.…”

Section: Introductionmentioning

confidence: 99%

“…However, different sizes of 0.1-5μm microparticles showed a size-dependent lethality with a significant decrease in the reproduction rate and brood size at 1μm microparticles. Yu et al 22 investigated the toxicity mechanisms of 1 μ m polystyrene microplastics on C. elegans at different concentrations of 0-100 μ g/L. Exposure to concentrations higher than 10μg/L induced a significant reduction in worms' head thrash, body bend, body length, and brood size.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic Electric Egg-Laying Assay and Application to In-vivo Toxicity Screening of Microplastics using C. elegans

Youssef

Archonta

Kubiseski

et al. 2021

Preprint

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Environmental pollutants like microplastics are posing health concerns on aquatic animals and the ecosystem. Microplastic toxicity studies using C. elegans as a model are evolving but methodologically hindered from obtaining statistically strong data sets, detecting toxicity effects based on microplastics uptake, and correlating physiological and behavioural effects at an individual-worm level. In this paper, we report a novel microfluidic electric egg-laying assay for phenotypical assessment of multiple worms in parallel. The effects of glucose and polystyrene microplastics at various concentrations on the worms’ electric egg-laying, length, diameter, and length contraction during exposure to electric signal were studied. The device contained eight parallel worm-dwelling microchannels called electric traps, with equivalent electrical fields, in which the worms were electrically stimulated for egg deposition and fluorescently imaged for assessment of neuronal and microplastic uptake expression. A new bidirectional stimulation technique was developed, and the device design was optimized to achieve a testing efficiency of 91.25%. Exposure of worms to 100mM glucose resulted in a significant reduction in their egg-laying and size. The effects of 1μm polystyrene microparticles at concentrations of 100 and 1000 mg/L on the electric egg-laying behaviour, size, and neurodegeneration of N2 and NW1229 (expressing GFP pan-neuronally) worms were also studied. Of the two concentrations, 1000 mg/L caused severe egg-laying deficiency and growth retardation as well as neurodegeneration. Additionally, using single-worm level phenotyping, we noticed intra-population variability in microplastics uptake and correlation with the above physiological and behavioural phenotypes, which was hidden in the population-averaged results. Taken together, these results suggest the appropriateness of our microfluidic assay for toxicological studies and for assessing the phenotypical heterogeneity in response to microplastics.

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Attenuative effects of tamarixetin against polystyrene microplastics‐induced hepatotoxicity in rats by regulation of Nrf‐2/Keap‐1 pathway

Ijaz,

Khalil,

Hamza

et al. 2023

Cell Biochemistry & Function

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Polystyrene microplastics (PS‐MPs) are environmental contaminants due to their potential to induce damages in multiple organs specifically liver. Tamarixetin (TMT) is a naturally occurring flavonoid present in Tamarix ramosissima plant that exhibits multiple pharmacological properties. Therefore, the present research was designed to evaluate the palliative role of TMT against PS‐MPs instigated liver dysfunction in rats. The exposure to PS‐MPs reduced the expressions of nuclear factor erythroid 2‐related factor 2 and antioxidant genes, while increasing the expression of Kelch‐like ECH‐associated protein 1. PS‐MPs exposed rats exhibited considerably (p < .05) higher alkaline phosphatase (ALP), aspartate aminotransferase (AST) as well as alanine aminotransferase (ALT) contents. Additionally, PS‐MPs treatment resulted in a notable decrease in anti‐oxidants activity, that is, glutathione S‐transferase (GST), superoxide dismutase (SOD), heme oxygenase‐1 (HO‐1), glutathione reductase (GSR), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH) content, whereas upregulating reactive oxygen species (ROS) and malondialdehyde (MDA) contents. Moreover, PS‐MPs intoxication noticeably increased (p < .05) the inflammatory indices (interleukin‐1ß [IL‐1ß], nuclear factor kappa B [NF‐κB], interleukin‐6 [IL‐6], tumor necrosis factor‐α [TNF‐α] levels, and cyclooxygenase‐2 [COX‐2] activity). Besides, Caspase‐3 and Bax expressions were upregulated and Bcl‐2 expression was decreased after PS‐MPs exposure. Additionally, the histomorphological examination revealed notable hepatic damage in PS‐MPs treated group. However, TMT treatment substantially (p < .05) recovered all the PS‐MPs‐induced damages and histopathological changes. Taken together, it can be deduced that TMT might be used as a pharmacological agent to ameliorate hepatic damage.

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Polystyrene microplastics (PS-MPs) toxicity induced oxidative stress and intestinal injury in nematode Caenorhabditis elegans

Cited by 124 publications

References 52 publications

Microplastic shape, concentration and polymer type affect soil properties and plant biomass

Microplastic shape, concentration and polymer type affect soil properties and plant biomass

Microfluidic Electric Egg-Laying Assay and Application to In-vivo Toxicity Screening of Microplastics using C. elegans

Attenuative effects of tamarixetin against polystyrene microplastics‐induced hepatotoxicity in rats by regulation of Nrf‐2/Keap‐1 pathway

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