Polystyrene microplastics induce gut microbiota dysbiosis and hepatic lipid metabolism disorder in mice

Liang, Lu; Wan, Zhiqin; Luo, Ting; Fu, Zhengwei; Jin, Yuanxiang

doi:10.1016/j.scitotenv.2018.03.051

Cited by 665 publications

(348 citation statements)

References 66 publications

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“…In the wild, due to food shortage, protein and fat intake decreased, and the Bacteroidetes content increased to help host to increase their nutrition. A disruption of the symbiosis between the microbiota and host is known as dysbiosis and is described in multiple chronic diseases, such as obesity and malnutrition (Castaner et al 2018;Zhang et al 2018;Jeong et al 2019), neurological disorders (Kurokawa et al 2018;Quagliariello et al 2018;Sun and Shen 2018), inflammatory bowel disease (IBD) (Costa et al 2012;Roche-Lima et al 2018), metabolic syndrome (Zhao et al 2018), cancer and other diseases (Katsimichas et al 2018;Lu et al 2018;Panebianco et al 2018;Pulikkan et al 2018;Zitvogel et al 2018). We presume that the health of the wild group of Tibetan wild asses was better than the captive group.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the gut microbiota composition between the wild and captive Tibetan wild ass ( Equus kiang )

et al. 2019

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Aims The gut microbiota has a great effect on the health and nutrition of the host. Manipulation of the intestinal microbiota may improve animal health and growth performance. The objectives of our study were to characterize the faecal microbiota between wild and captive Tibetan wild asses and discuss the differences and their reasons. Methods and Results Through high‐throughput sequencing of the 16S rRNA V4‐V5 region, we studied the gut microbiota composition and structure of Tibetan wild asses in winter, and analysed the differences between wild and captive groups. The results showed that the most common bacterial phylum in Tibetan wild ass faeces samples was Bacteroidetes, while the phylum Firmicutes was dominant in captive Tibetan wild ass faecal samples. The relative abundance of Firmicutes, Tenericutes and Spirochaetes were significantly higher (P < 0·01) than in the wild groups. Conclusions Captivity reduces intestinal microbial diversity, evenness and operational taxonomic unit number due to the consumption of industrial food, therefore, increasing the risk of disease prevalence and affecting the health of wildlife. Significance and Impact of the Study We studied the effect of the captive environment on intestinal micro‐organisms. This article provides a theoretical basis for the ex‐situ conservation of wild animals in the future.

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Section: Discussionmentioning

confidence: 99%

Comparison of the gut microbiota composition between the wild and captive Tibetan wild ass ( Equus kiang )

et al. 2019

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“…Oxidative stress is tightly associated with hepatic toxicity and is often caused by environmental heavy metals. Metal‐induced alterations in antioxidant enzyme activities have been extensively studied . Pb has been found to derail the antioxidant defense system by interfering with the metals that are essential for antioxidant enzyme activities, supporting the role of oxidative stress in lead hepatotoxicity .…”

Section: Discussionmentioning

confidence: 99%

“…Real‐time polymerase chain reaction (RT‐qPCR) was performed in an Eppendorf Master Cyclerep RealPlex2 (Wesseling‐Berzdorf, Germany) tube. Oligonucleotide primers used were obtained from previous studies . Samples were normalized to GAPDH levels.…”

Section: Methodsmentioning

confidence: 99%

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Chronic exposure to low doses of Pb induces hepatotoxicity at the physiological, biochemical, and transcriptomic levels of mice

Luo

Shen

Zhou

et al. 2019

Environmental Toxicology

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Lead (Pb), a non-essential heavy metal, is a major global environmental contaminant with serious toxicological consequences. In the present study, the effects on hepatotoxicity of mice with chronic exposure to low doses of Pb were evaluated. While oral exposure to 0.03 or 0.1 mg/L Pb for 15 weeks in male adult mice had no significant effect on body weights, Pb exposure resulted in liver histopathological effects and increase of hepatic activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). In addition, hepatic reactive oxygen species (ROS) and malondialdehyde (MDA) significantly accumulated after treatment. Conversely, glutathione (GSH) decreased significantly in both 0.03 and 0.1 mg/L Pb-treated groups. Moreover, the hepatic activities of superoxide dismutase 1 (SOD) and catalase (CAT) increased significantly following treatment with 0.1 mg/L Pb for 15 weeks, concomitant with increases in transcriptions of hepatic Sod, Cat, and Gpx. Furthermore, transcriptions of hepatic metallothionein (MT), zinc transporter 5 (Znt5) and copper transporter 1 (Ctr1), and subsequent protein levels were also increased in liver of mice when exposed to 0.1 mg/L Pb for 15 weeks. In addition, the transcriptome data showed that Pb has substantial influence on several pathways, including PPAR signaling pathways, AMPK signaling pathways, fatty acid metabolism, and drug metabolism. Our data suggested that chronic Pb exposure could induce hepatotoxicity at the physiological, biochemical, and transcriptomic levels in mice. K E Y W O R D Shepatotoxicity, oxidative stress, gene expression, Pb

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“…The gene transcription was normalized to the level of GAPDH transcript in each sample. The following PCR protocol was used as denaturation for 1 minute at 95°C, followed by 40 cycles of 15 seconds at 95°C and 1 minute at 60°C according to previous studies …”

Section: Methodsmentioning

confidence: 99%

Propamocarb exposure decreases the secretion of neurotransmitters and causes behavioral impairments in mice

Zhang

Jin

Wang

et al. 2018

Environmental Toxicology

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Propamocarb (PM) is a type of fungicide, which is widely used in the greenhouse-based production of vegetables and fruits globally. It has been considered to have generally low toxicity. However, the teratogenicity or neurotoxicity for mammals remains unclear. In this study, we aimed to explore its effect on the secretion of neurotransmitters and behavioral impairments.Male adult mice were exposed to 10 and 40 mg/L PM for 2 weeks (acute exposure) or 3 and 10 mg/L PM for 10 weeks (chronic exposure). It was observed that acute or chronic exposure to PM changed the levels of serotonin (5-HT) and dopamine in the serum and colon and the transcription of TPH2 and DRD2 in the colons of mice. In addition, the locomotor test, the open field test, and the Morris water maze analysis also showed that acute exposure to PM caused behavioral impairments to some extent. The results obtained in the present study indicated that PM has the potential to induce neurotoxicity in animals. K E Y W O R D Sbehavioral impairments, mice, neurotoxicity, neurotransmitters, propamocarb

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Polystyrene microplastics induce gut microbiota dysbiosis and hepatic lipid metabolism disorder in mice

Cited by 665 publications

References 66 publications

Comparison of the gut microbiota composition between the wild and captive Tibetan wild ass ( Equus kiang )

Comparison of the gut microbiota composition between the wild and captive Tibetan wild ass ( Equus kiang )

Chronic exposure to low doses of Pb induces hepatotoxicity at the physiological, biochemical, and transcriptomic levels of mice

Propamocarb exposure decreases the secretion of neurotransmitters and causes behavioral impairments in mice

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