Polystyrene microplastics effects on zebrafish embryological development: Comparison of two different sizes

Insight on cytotoxic NHC gold(I) halide complexes evaluated in multifaceted culture systems

“…At 6 h post fertilization (hpf), four groups with ten embryos each one, in duplicate (20 embryos for group in total), were set up in 6-well microplates in according to La Pietra et al, (2024) ; three groups were treated with 10 mL of Au4Mc 5.53 μM, Au6Mc 5.03 μM or DMSO 0.01 %, respectively, and one group was exposed to only E3 medium as control group. Embryos treated were incubated under static conditions and monitored up to 72 h (h) of treatment.…”

“…The use of chemotherapeutics during pregnancy, breeding, or at a young age could pose a risk, so, it is important to test their toxicity in vivo during these stages ( Gao et al, 2017 ). Zebrafish, a vertebrate approximately 71 % genetically identical to humans ( Howe et al, 2013 ), is an excellent model organism that lends itself well to the study of the toxicity of new compounds or pollutants in both medical ( Hung et al, 2019 , Van Sebille et al, 2019 ) and ecotoxicological fields ( Capriello et al, 2019 , Capriello et al, 2021 , La Pietra et al, 2024 ). Its embryos are used for embryotoxicity testing because of rapid reproduction, transparency, and rapid development ( Yang et al, 2009 ).…”

Comparative Study of Condensed and Hydrolysable Tannins during Early Stages of Zebrafish Development

“…The treatments were carried out in 6-well plates at 28.0 °C with 10 embryos per well adding 10 mL of solution that was renewed every day [68]. A total of 20 embryos were used for each group [69] and the experiments were all triplicated according to the principle of 3Rs (Replacement, Reduction and Refinement) (3Rs principles) [70], to limit the overuse of animals.…”