Polysome Profiling Shows the Identity of Human Adipose-Derived Stromal/Stem Cells in Detail and Clearly Distinguishes Them from Dermal Fibroblasts

Zych, Jaiesa; Spangenberg, Lucía; Stimamiglio, Marco Augusto; Abud, Ana Paula Ressetti; Shigunov, Patrícia; Marchini, Fabrício Klerynton; Kuligovski, Crisciele; Cofré, Axel R.; Schittini, Andressa V.; Aguiar, Alessandra Melo de; Senegaglia, Alexandra Cristina; Brofman, Paulo Roberto Slud; Goldenberg, Samuel; Dallagiovanna, Bruno; Naya, Hugo; Correa, Alejandro

doi:10.1089/scd.2013.0496

Cited by 9 publications

(11 citation statements)

References 42 publications

(58 reference statements)

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“…Mechanisms for fatty metaplasia have been proposed including cell damage with intracytoplasmic lipid accumulation and differentiation from facultative fibroblasts. More recent stem cell investigations have identified dermal stem cells distinctive from dermal fibroblasts that may be stimulated to differentiate to adipocytes …”

Section: Discussionmentioning

confidence: 99%

Lipomatous metaplasia of superficial dermis

Tidwell

Rosenberg

2015

J Cutan Pathol

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Lipomatous metaplasia is an uncommon phenomenon. After identifying the presence of a band of adipocytes in the superficial reticular dermis underlying two excisions for basal cell carcinoma, we prospectively reviewed all skin specimens accessioned in our laboratory over a 6-month period and identified eight additional cases. In each example there was a band of adipocytes in the upper dermis, at the level of solar elastosis that was widely separated from the subcutaneous fat by a normal appearing reticular dermis. The cells were positive for S100 and negative for CD163. No connection between the superficial band of adipocytes and the subcutaneous or periappendageal fat was seen. The alterations were flat in configuration without polypoid changes. Eyerich et al. reported lipomatous metaplasia in the dermis of a patient with acute generalized exanthematic pustulosis and psoriasis, and postulated this to be a postinflammatory phenomenon. Fatty metaplasia occurs within a variety of cutaneous neoplasms including nevi, adnexal tumors and peripheral nerve sheath tumors. However, superficial dermal fatty metaplasia beneath cutaneous neoplasms is a newly described phenomenon and we suspect this process represents fatty metaplasia within solar elastosis and that it may occur more frequently than recognized.

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Section: Discussionmentioning

confidence: 99%

Lipomatous metaplasia of superficial dermis

Tidwell

Rosenberg

2015

J Cutan Pathol

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“…After induction of osteogenic differentiation for 24 h, cells were trypsinized, plated on 24-well plates (2,5 × 10 4 cells/well) and kept for 10, 20 and 40 min in a humidified incubator at 37 °C with 5% CO 2 as previously described 56 . The non-adherent cells were removed by washing twice with PBS.…”

Section: Methodsmentioning

confidence: 99%

Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis

Robert

Angulski

Spangenberg

et al. 2018

Sci Rep

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Mesenchymal stem cells (MSCs) have been widely studied with regard to their potential use in cell therapy protocols and regenerative medicine. However, a better comprehension about the factors and molecular mechanisms driving cell differentiation is now mandatory to improve our chance to manipulate MSC behavior and to benefit future applications. In this work, we aimed to study gene regulatory networks at an early step of osteogenic differentiation. Therefore, we analyzed both the total mRNA and the mRNA fraction associated with polysomes on human adipose tissue-derived stem cells (hASCs) at 24 h of osteogenesis induction. The RNA-seq results evidenced that hASC fate is not compromised with osteogenesis at this time and that 21 days of continuous cell culture stimuli are necessary for full osteogenic differentiation of hASCs. Furthermore, early stages of osteogenesis induction involved gene regulation that was linked to the management of cell behavior in culture, such as the control of cell adhesion and proliferation. In conclusion, although discrete initial gene regulation related to osteogenesis occur, the first 24 h of induction is not sufficient to trigger and drive in vitro osteogenic differentiation of hASCs.

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“…A classical technique called polysome profiling has been used to assess the mRNAs bound to ribosomes through ultracentrifugation in a sucrose gradient, and further identify them by high-throughput methods [ 23 – 25 ]. Previously, we used polysome profiling to study the fate of human adipose stem cells (hASCs) and their commitment to adipogenesis [ 25 , 26 ]. We showed that 60% of the genes, which were differentially expressed after 72 h of differentiation induction, were controlled by post-transcriptional regulation.…”

Section: Introductionmentioning

confidence: 99%

Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes

et al. 2019

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BackgroundCardiac cell fate specification occurs through progressive steps, and its gene expression regulation features are still being defined. There has been an increasing interest in understanding the coordination between transcription and post-transcriptional regulation during the differentiation processes. Here, we took advantage of the polysome profiling technique to isolate and high-throughput sequence ribosome-free and polysome-bound RNAs during cardiomyogenesis.ResultsWe showed that polysome-bound RNAs exhibit the cardiomyogenic commitment gene expression and that mesoderm-to-cardiac progenitor stages are strongly regulated. Additionally, we compared ribosome-free and polysome-bound RNAs and found that the post-transcriptional regulation vastly contributes to cardiac phenotype determination, including RNA recruitment to and dissociation from ribosomes. Moreover, we found that protein synthesis is decreased in cardiomyocytes compared to human embryonic stem-cells (hESCs), possibly due to the down-regulation of translation-related genes.ConclusionsOur data provided a powerful tool to investigate genes potentially controlled by post-transcriptional mechanisms during the cardiac differentiation of hESC. This work could prospect fundamental tools to develop new therapy and research approaches.Electronic supplementary materialThe online version of this article (10.1186/s12864-019-5550-3) contains supplementary material, which is available to authorized users.

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Polysome Profiling Shows the Identity of Human Adipose-Derived Stromal/Stem Cells in Detail and Clearly Distinguishes Them from Dermal Fibroblasts

Cited by 9 publications

References 42 publications

Lipomatous metaplasia of superficial dermis

Lipomatous metaplasia of superficial dermis

Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis

Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes

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