Polysialic acid biosynthesis and production in Escherichia coli: current state and perspectives

Lin, Bingcheng; Qiao, Yu; Shi, Bo; Tao, Yong

doi:10.1007/s00253-015-7019-x

Cited by 69 publications

(60 citation statements)

References 65 publications

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“…Fourthly, unclassified bacteria in the nine samples accounted for 18.82–22.48% of sequences. According to previous studies, many animal gut microbiomes contained certain proportions of unclassified bacteria (Bian et al 2013; Costa et al 2012; Wu et al 2016). The reason of this might due to the limited database of 16S RNA gene sequences and little research on classification of fecal microbes.…”

Section: Discussionmentioning

confidence: 93%

“…Previous researches also showed that the fecal microbiota of many mammals might be influenced by sex (Bian et al 2013; Liu et al 2014; Wu et al 2016). In present study, the four female golden takins demonstrated highly similar microbiota and the five golden takin males had relatively highly similar microbiota, although J6 and J7 were relatively far from the other three samples, which might indicate that the fecal microbiota of golden takins might also be influenced by the animal’s sex.…”

Section: Discussionmentioning

confidence: 98%

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Characterization of the gut microbiota in the golden takin (Budorcas taxicolor bedfordi)

Zhang

Shang

et al. 2017

AMB Expr

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The gut microbiota of mammals is a complex ecosystem, which is essential for maintaining gut homeostasis and the host’s health. The high throughput sequencing allowed us to gain a deeper insight into the bacterial structure and diversity. In order to improve the health status of the endangered golden takins, we first characterized the fecal microbiota of healthy golden takins using high throughput sequencing of the 16S rRNA genes V3–V4 hypervariable regions. Our results showed that, Firstly, the gut microbiota community comprised 21 phyla, 40 classes, 62 orders, 96 families, and 216 genera. Firmicutes (67.59%) was the most abundant phylum, followed by Bacteroidetes (23.57%) and Proteobacteria (2.37%). Secondly, the golden takin maintained higher richness in spring than in the winter while community diversity and evenness was not significantly different. Thirdly, four female golden takins demonstrated highly similar microbiota and the five golden takin males had relatively highly similar microbiota. All of our results might indicate that the fecal microbiota of golden takins were influenced by the season and the animal’s sex. The findings provided theoretical basis regarding the gut microbiota of golden takins and may offer new insights to protect this endangered species.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Characterization of the gut microbiota in the golden takin (Budorcas taxicolor bedfordi)

Zhang

Shang

et al. 2017

AMB Expr

View full text Add to dashboard Cite

show abstract

“…For example, Neu5Ac is the precursor of polysialic acid (PSA) and sialylated oligosaccharides. Thus, an enhanced Neu5Ac biosynthetic module can also improve production of PSA and sialylated oligosaccharides [41, 42]. Whole-cell biocatalyst that was designed for Neu5Ac has been used to produce 11 Neu5Ac derivatives using chemically modified GlcNAc analogues as substrates [16, 43].…”

Section: Whole-cell Biocatalyst Design Principlesmentioning

confidence: 99%

“…Several considerations influence the optimal growth of the biocatalyst, as with any fermentation, but the major issues regarding their use in biotransformations are the expression level(s) of the enzyme(s) of interest and the biomass yield. For most whole-cell biocatalysts that contain multistep pathways, coordinated expression, but not necessarily overexpression of the many enzymes involved in the pathways, is of great importance [16, 41, 49, 51]. A good balance is a prerequisite for the efficiency of the biocatalyst.…”

Section: Process Engineeringmentioning

confidence: 99%

Whole-cell biocatalysts by design

2017

Self Cite

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Whole-cell biocatalysts provide unique advantages and have been widely used for the efficient biosynthesis of value-added fine and bulk chemicals, as well as pharmaceutically active ingredients. What is more, advances in synthetic biology and metabolic engineering, together with the rapid development of molecular genetic tools, have brought about a renaissance of whole-cell biocatalysis. These rapid advancements mean that whole-cell biocatalysts can increasingly be rationally designed. Genes of heterologous enzymes or synthetic pathways are increasingly being introduced into microbial hosts, and depending on the complexity of the synthetic pathway or the target products, they can enable the production of value-added chemicals from cheap feedstock. Metabolic engineering and synthetic biology efforts aimed at optimizing the existing microbial cell factories concentrate on improving heterologous pathway flux, precursor supply, and cofactor balance, as well as other aspects of cellular metabolism, to enhance the efficiency of biocatalysts. In the present review, we take a critical look at recent developments in whole-cell biocatalysis, with an emphasis on strategies applied to designing and optimizing the organisms that are increasingly modified for efficient production of chemicals.

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“…Thus, engineering transporters from both microorganisms and plants can improve the production of PNPs in microbial hosts [54, 55]. …”

Section: Optimization Of Microbial Cell Factoriesmentioning

confidence: 99%

Engineering microbial cell factories for the production of plant natural products: from design principles to industrial-scale production

2017

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Plant natural products (PNPs) are widely used as pharmaceuticals, nutraceuticals, seasonings, pigments, etc., with a huge commercial value on the global market. However, most of these PNPs are still being extracted from plants. A resource-conserving and environment-friendly synthesis route for PNPs that utilizes microbial cell factories has attracted increasing attention since the 1940s. However, at the present only a handful of PNPs are being produced by microbial cell factories at an industrial scale, and there are still many challenges in their large-scale application. One of the challenges is that most biosynthetic pathways of PNPs are still unknown, which largely limits the number of candidate PNPs for heterologous microbial production. Another challenge is that the metabolic fluxes toward the target products in microbial hosts are often hindered by poor precursor supply, low catalytic activity of enzymes and obstructed product transport. Consequently, despite intensive studies on the metabolic engineering of microbial hosts, the fermentation costs of most heterologously produced PNPs are still too high for industrial-scale production. In this paper, we review several aspects of PNP production in microbial cell factories, including important design principles and recent progress in pathway mining and metabolic engineering. In addition, implemented cases of industrial-scale production of PNPs in microbial cell factories are also highlighted.

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Polysialic acid biosynthesis and production in Escherichia coli: current state and perspectives

Cited by 69 publications

References 65 publications

Characterization of the gut microbiota in the golden takin (Budorcas taxicolor bedfordi)

Characterization of the gut microbiota in the golden takin (Budorcas taxicolor bedfordi)

Whole-cell biocatalysts by design

Engineering microbial cell factories for the production of plant natural products: from design principles to industrial-scale production

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