Polysaccharides on gelatin-based hydrogels differently affect chondrogenic differentiation of human mesenchymal stromal cells

Sartore, Luciana; Manferdini, Cristina; Saleh, Yasmin; Dey, Kamol; Gabusi, Elena; Ramorino, Giorgio; Zini, Nicoletta; Almici, Camillo; Re, Federica; Russo, Domenico; Mariani, Erminia; Lisignoli, Gina

doi:10.1016/j.msec.2021.112175

Cited by 20 publications

(17 citation statements)

References 48 publications

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“…Within tissue, cells always go through a dynamic microenvironment [ 24 ]. Numerous recent works revealed how time-dependent response (viscoelasticity) of scaffolds modulated stem cell behavior and stated that faster stress relaxing scaffolds promoted stem cell proliferation and differentiation towards specific tissue [ 5 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. All these outcomes warrant an in-depth study of mechanical properties of the hydrogel before a biological test using stem cells and motivated performance of the present study.…”

Section: Introductionmentioning

confidence: 99%

Degradation-Dependent Stress Relaxing Semi-Interpenetrating Networks of Hydroxyethyl Cellulose in Gelatin-PEG Hydrogel with Good Mechanical Stability and Reversibility

Dey

Agnelli

Borsani

et al. 2021

Gels

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The mechanical milieu of the extracellular matrix (ECM) plays a key role in modulating the cellular responses. The native ECM exhibits viscoelasticity with stress relaxation behavior. Here, we reported the preparation of degradation-mediated stress relaxing semi-interpenetrating (semi-IPN) polymeric networks of hydroxyethyl cellulose in the crosslinked gelatin-polyethylene glycol (PEG) architecture, leveraging a newly developed synthesis protocol which successively includes one-pot gelation under physiological conditions, freeze-drying and a post-curing process. Fourier transform infrared (FTIR) confirmed the formation of the semi-IPN blend mixture. A surface morphology analysis revealed an open pore porous structure with a compact skin on the surface. The hydrogel showed a high water-absorption ability (720.00 ± 32.0%) indicating the ability of retaining a hydrophilic nature even after covalent crosslinking with functionalized PEG. Detailed mechanical properties such as tensile, compressive, cyclic compression and stress relaxation tests were conducted at different intervals over 28 days of hydrolytic degradation. Overall, the collective mechanical properties of the hydrogel resembled the mechanics of cartilage tissue. The rate of stress relaxation gradually increased with an increasing swelling ratio. Hydrolytic degradation led to a marked increase in the percentage dissipation energy and stress relaxation response, indicating the degradation-dependent viscoelasticity of the hydrogel. Strikingly, the hydrogel maintained the structural stability even after degrading two-thirds of its initial mass after a month-long hydrolytic degradation. This study demonstrates that this semi-IPN G-PEG-HEC hydrogel possesses bright prospects as a potential scaffolding material in tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Degradation-Dependent Stress Relaxing Semi-Interpenetrating Networks of Hydroxyethyl Cellulose in Gelatin-PEG Hydrogel with Good Mechanical Stability and Reversibility

Dey

Agnelli

Borsani

et al. 2021

Gels

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“…In addition, vibration bands corresponding to the C–O–C (at 1082 cm –1 ) and C–O–H (shoulder at 1114 cm –1 ) functional groups dramatically increased for the cross-linked films. The significant improvement in these peaks implies that the epoxy rings of the PEGDE is taking part in the cross-linking reaction. ,− Further confirmation of chemical cross-linking was conducted by simple dissolution tests, where the non-cross-linked Col:Gel films immediately dissolves upon immersion into PBS buffer solutions (pH 7.4, 10 mM), but the cross-linked counterparts remain intact for at least 10 days.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, vibration bands corresponding to the C−O−C (at 1082 cm −1 ) and C−O−H (shoulder at 1114 cm −1 ) functional groups dramatically increased for the cross-linked films. The significant improvement in these peaks implies that the epoxy rings of the PEGDE is taking part in the cross-linking reaction 24,[37][38][39].…”

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confidence: 99%

Bioinspired Collagen/Gelatin Nanopillared Films as a Potential Implant Coating Material

Erturk

Altuntaş

Irmak

et al. 2022

ACS Appl. Bio Mater.

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Collagen-based Sharpey's fibers are naturally located between alveolar bone and tooth, and they have critical roles in a well-functioning tooth such as mechanical stability, facile differentiation, and disease protection. The success of Sharpey's fibers in these important roles is due to their unique location, vertical alignment with respect to tooth surface, as well as their micronanofiber architecture. Inspired by these structures, herein, we introduce the use of nanoporous anodic aluminum oxide molds in a drop-casting setup to fabricate biopolymeric films possessing arrays of uniform Collagen:Gelatin (Col:Gel) nanopillars. Obtained structures have diameters of ∼90 nm and heights of ∼300 nm, yielding significantly higher surface roughness values compared to their flat counterparts. More importantly, the nanostructures were parallel to each other but perpendicular to the underlying film surface imitating the natural collagenous structures of Sharpey's fibers regarding nanoscale morphology, geometrical orientation, as well as biochemical content. Viability testing showed that the nanopillared Col:Gel films have high cell viabilities (over 90%), and they display significantly improved attachment (ca. ∼ 2 times) and mineralization for Saos-2 cells when compared to flat Col:Gel films and Tissue Culture Polystyrene (TCPS) controls, plausibly due to their largely increased surface roughness and area. Hence, such Sharpey's fiber-inspired bioactive nanopillared Col:Gel films can be used as a dental implant coating material or tissue engineering platform with enhanced cellular and osteogenic properties.

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“…Viscoelasticity is the capacity of a hydrogel to exhibit both viscous and elastic behavior following the application of force [ 27 ]. Moreover, microstructural and spatial hydrogel properties such as porosity and anisotropy (hydrogel with well oriented structure) that create the architecture of the hydrogels also significantly influence the chondrogenic differentiation of MSCs [ 18 , 28 , 29 ]. Finally, it has been demonstrated that functionalized hydrogels with peptide or nanoparticles show positive effects on chondrogenic differentiation, with the ability to regulate cell activity and to show a tunable biodegradation profile [ 30 , 31 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stromal Cells Laden in Hydrogels for Osteoarthritis Cartilage Regeneration: A Systematic Review from In Vitro Studies to Clinical Applications

Manferdini

Gabusi

Saleh

et al. 2022

Cells

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This systematic review is focused on the main characteristics of the hydrogels used for embedding the mesenchymal stromal cells (MSCs) in in vitro/ex vivo studies, in vivo OA models and clinical trials for favoring cartilage regeneration in osteoarthritis (OA). PubMED and Embase databases were used to select the papers that were submitted to a public reference manager Rayyan Systematic Review Screening Software. A total of 42 studies were considered eligible: 25 articles concerned in vitro studies, 2 in vitro and ex vivo ones, 5 in vitro and in vivo ones, 8 in vivo ones and 2 clinical trials. Some in vitro studies evidenced a rheological characterization of the hydrogels and description of the crosslinking methods. Only 37.5% of the studies considered at the same time chondrogenic, fibrotic and hypertrophic markers. Ex vivo studies focused on hydrogel adhesion properties and the modification of MSC-laden hydrogels subjected to compression tests. In vivo studies evidenced the effect of cell-laden hydrogels in OA animal models or defined the chondrogenic potentiality of the cells in subcutaneous implantation models. Clinical studies confirmed the positive impact of these treatments on patients with OA. To speed the translation to the clinical use of cell-laden hydrogels, further studies on hydrogel characteristics, injection modalities, chemo-attractant properties and adhesion strength are needed.

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Polysaccharides on gelatin-based hydrogels differently affect chondrogenic differentiation of human mesenchymal stromal cells

Cited by 20 publications

References 48 publications

Degradation-Dependent Stress Relaxing Semi-Interpenetrating Networks of Hydroxyethyl Cellulose in Gelatin-PEG Hydrogel with Good Mechanical Stability and Reversibility

Degradation-Dependent Stress Relaxing Semi-Interpenetrating Networks of Hydroxyethyl Cellulose in Gelatin-PEG Hydrogel with Good Mechanical Stability and Reversibility

Bioinspired Collagen/Gelatin Nanopillared Films as a Potential Implant Coating Material

Mesenchymal Stromal Cells Laden in Hydrogels for Osteoarthritis Cartilage Regeneration: A Systematic Review from In Vitro Studies to Clinical Applications

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