Polysaccharides of Spina Gleditsiae, A Potential Treasure in Anticancer and Antioxidant Drug Discovery

Zhao

et al. 2021

Food Sci. Technol

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Galla Chinensis was a traditional Chinese medicine used as an ingredient in many prescriptions for the treatment of oxidant damage-associated symptoms in China. The current research on the isolation of polysaccharides from Galla Chinensis and their antioxidant activities still remain as an ongoing challenge. The purpose of this study is to develop an isolation technique for polysaccharides from Galla Chinensis and determine their antioxidant properties in vitro. An ultrasonic-assisted method of polysaccharides extraction from Galla Chinensis were firstly developed and the key parameters were optimized response surface analysis as crushing degree 100 mesh, ultrasonic time 20 min, and materials/liquid ratio 1: 40. The polysaccharides presented dose-dependent antioxidant and free radical scavenging effects in DPPH, ABTS, total antioxidant and Iron ion reduction assays. Moreover, it also demonstrated an effective protective effect for human under H 2 O 2 -induced oxidative damage on human embryonic kidneyfibroblast 293 and hepatoma 7721 cells by cell proliferation assay. The study firstly determined the antioxidant activities of polysaccharides from Galla Chinensis, thus providing a new natural resource for future investigation and development of the polysaccharides-based antioxidant drugs, health products or addictives.

Section: Introductionmentioning

confidence: 99%

Response surface optimization of polysaccharide extraction from Galla Chinensis and determination of its antioxidant activity in vitro

Zhao

et al. 2021

Food Sci. Technol

Self Cite

“…8,9 Previously, triterpenoid saponins and polysaccharides had been found to possess great anticancer activities on cancer cell lines in vitro. 9,10 However, the chemical foundation underlying the anticancer activities of Spina Gleditsiae has not been systematically investigated as yet, and an overall perspective on its anticancer phytochemical foundation still remains an ongoing challenge. The multivariate data analysis (MVA)guided method, developed recently and characterized as minimum consuming of time, raw materials, and solvents, could provide an advanced technique for the fast screening of bioactive agents of complex medicinal products.…”

mentioning

confidence: 99%

“…7 The functional compounds, belonging to 5 major groups of secondary metabolites (triterpenes, sterols, flavonoids, phenolic, and alkaloids), have been isolated and elucidated from Spina Gleditsiae. 8,9 Previously, triterpenoid saponins and polysaccharides had been found to possess great anticancer activities on cancer cell lines in vitro. 9,10 However, the chemical foundation underlying the anticancer activities of Spina Gleditsiae has not been systematically investigated as yet, and an overall perspective on its anticancer phytochemical foundation still remains an ongoing challenge.…”

mentioning

confidence: 99%

Multivariate Data Analysis Guided Identification of Anticancer Constituents of Spina Gleditsiae for Human Hepatoma Cells

Natural Product Communications

Chen

et al. 2020

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Spina Gleditsia has been traditionally used as a lead ingredient of many formulas for cancer treatment in Traditional Chinese Medicine. This work aimed to reveal the phytochemical foundation of Spina Gleditsiae and underpin a fundamental understanding of its anticancer activities. The growth inhibition activities were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay of 7 solvent extracts of Spina Gleditsia in the human liver cancer cell line HepG2. The activities were then mapped onto the secondary metabolite profile of crude extracts by principal components analysis (PCA) of High Performance Liquid Chromatography-Ultraviolet (HPLC-UV) data. The top 5 PCA components of the map discriminated extract activities, mainly based on the differential content of 3 compounds. The identified compounds were then tested individually using cytotoxic assay on cancer cells in vitro. Principal components analysis constructed secondary metabolite mapping quickly identified functional compounds from the crude herbal extracts. (2 R,3 R)-7,3′,4′-Trihydroxy-5-methoxy-flavanonol, (2 R,3 R)-5,7,3′-trihydroxy-4′-methoxy-flavanonol, and isovitexin inhibited the growth of human liver cancer cell line growth with activities of 38.6 ± 1.9, 40.7 ± 0.9, and 41.5 ± 3.1 μg/mL, respectively, comparable with that of cisplatin (IC50: 41.6 ± 2.9 μg/mL). (2 R,3 R)-7,3′,4′-Trihydroxy-5-methoxy-flavanonol, (2 R,3 R)-5,7,3′-trihydroxy-4′-methoxy-flavanonol, and isovitexin are, for the first time, identified as the anticancer constituents of Spina Gleditsia. It thus strongly underpins a fundamental understanding of the anticancer activities of Spina Gleditsia and supports its ongoing clinical uses in China.

“…14 They thus have been identified as the main antioxidant ingredient of many herbal medicines. [15][16][17][18] However, the antioxidant effects of Galla Turcica polysaccharides were barely reported. To underpin a fundamental understanding of bioactive materials and antioxidant activities of Galla Turcica and support its ongoing clinical uses in China, the aim of the study was to develop and optimize an ultrasonic-assisted extraction method by response surface analysis for polysaccharides isolation from Galla Turcica.…”

mentioning

confidence: 99%

Extraction Optimization of Galla Turcica Polysaccharides and Determination of Its Antioxidant Activities In Vitro

Natural Product Communications

Zhao

et al. 2020

Self Cite

Though natural polysaccharides commonly show antioxidant activities, the current research on the isolation of polysaccharides from Galla Turcica and their antioxidant activities still remain as an ongoing challenge. In this work, response surface analysis was employed to optimize an ultrasonic-assisted extraction method for polysaccharides of Galla Turcica. Their antioxidant and free radical scavenging activities were then evaluated using 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), total antioxidant activity, and iron ion reduction assays. Moreover, the protective effects of polysaccharides of Galla Turcica were determined on human embryonic kidney fibroblast 293 and hepatoma 7721 cells by cell proliferation assay. Overall, the key parameters of Galla Turcica polysaccharides extraction were optimized as crushing degree 100 mesh, ultrasonic time 50 min, and materials–liquid ratio 1:50. The isolated polysaccharides presented dose-dependent antioxidant and free radical scavenging effects in vitro. It also demonstrated an effective protective effect for human cells under oxidative damage. The results firstly determined the antioxidant activities of polysaccharides from Galla Turcica, thus providing a new natural resource for future investigation and development of the polysaccharides-based antioxidant drugs, health products, or additives.