Polysaccharides isolated from Cordyceps Sinensis contribute to the progression of NASH by modifying the gut microbiota in mice fed a high-fat diet

Chen, Lei; Zhang, Liangyu; Wang, Wendong; Qiu, Wei; Liu, Lei; Ning, Anhong; Cao, Jing; Huang, Min; Zhong, Ming-Chao

doi:10.1371/journal.pone.0232972

Cited by 27 publications

(20 citation statements)

References 81 publications

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“…The changes in the gut microbiota are related with speci c metabolic states, such as obesity [40]. Actinobacteria was positively correlated with obesity [41], the high level of Actinobacteria aggravated the disorder of the intestinal ora and contributed to the progression from obesity to the related diseases [42]. Saccharibacteria are prominent in the oral cavity, and increased abundance of Saccharibacteria is correlated with the development of periodontitis [43] and in ammatory bowel disease [44].…”

Section: Discussionmentioning

confidence: 99%

Evaluation on the characteristics of gut microbiome in polycystic ovary syndrome rats induced by dihydrotestosterone or letrozole

Zheng

Xü

et al. 2021

Preprint

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Background: Etiology of polycystic ovary syndrome (PCOS) is unclear. Recent reports indicated that gut microbiota regulates metabolism and plays a major role in the development of PCOS. The present study aimed to investigate and compare the effects of dihydrotestosterone or letrozole on the gut microbiome in rats.Methods: We used dihydrotestosterone (DHT) or letrozole (LET) to induce PCOS model rats. At the end of the experiment, ovarian morphology, hormonal and metabolic status were investigated in all rats. We analyzed the gut microbiome of rats by deep sequencing the 16S rDNA V3-V4 hypervariable regions. Predictive metagenomics profiling using PICRUST revealed functional alternation of the KEGG pathways in rats.Result: Rats induced by letrozole exhibited endocrine and reproductive characteristics, such as hyperandrogenism, abnormal oestrus cycles or complete acyclic, polycystic ovaries, and obesity. DHT-induced rats were obesity, irregular oestrus cycles, polycystic ovaries, with lower level of HDL-C and lower activity of SOD than controls. The bacterial diversity (Chao1 and Ace) of DHT group was decreased compared with the control group. Furthermore, principal coordinates analysis revealed that DHT and control groups could be distinguished from each other. The DHT group exhibited increased numbers of Firmicutes, Cyanobacteria, Actinobacteria and Saccharibacteria, and decreased Bacteroidetes compared with the control group at the phylum level. Moreover, PICRUST-predicted KEGG pathways related to Carbohydrate metabolism, Amino acid metabolism, Metabolism of terpenoids and polyketides, Metabolism of other amino acids, Replication and repair, Lipid metabolism and Energy metabolism, Translation, Folding, sorting and degradation and Xenobiotics biodegradation and metabolism were significantly elevated in the DHT group. And pathways related to Metabolism of terpenoids and polyketides, Replication and repair, Xenobiotics biodegradation and metabolism, Nucleotide metabolism, Infectious diseases and Excretory system were significantly elevated in the LET group.Conclusion: The findings of the present study indicated that DHT affected the composition and diversity of gut microbial community, and leads to the gut dysbiosis. KEGG function profiles calculated by PICRUST suggested that DHT may affect metabolism of the gut microbiota more directly than letrozle.

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Section: Discussionmentioning

confidence: 99%

Evaluation on the characteristics of gut microbiome in polycystic ovary syndrome rats induced by dihydrotestosterone or letrozole

Zheng

Xü

et al. 2021

Preprint

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“…CSP1-2 fraction also demonstrated an antihypertensive effect on spontaneously hypertensive rats (SHR) by stimulating the secretion of vasodilator NO, decreasing the level of ET-1, epinephrine, noradrenaline, and angiotensin II, inhibiting the increase of transforming growth factor β1 (TGF-β1) and lowering the level of inflammatory mediator of C-reactive protein (CRP) [94]. CSP soluble dietary fiber had protective effects against obesity on HFD-feeding C57BL/6J mice; however, the study also showed that, though CSP could prevent the increase in body weight, it could also result in aggravated liver fibrosis and steatosis [98]. CSP has also been shown to improve microbial community diversity and modulated the overall structure of gut microbiota, thus can be a potential prebiotic agent [96,97].…”

Section: Mushroom Species Containing Bioactive Polysaccharidesmentioning

confidence: 99%

Macrofungi as a Nutraceutical Source: Promising Bioactive Compounds and Market Value

Niego

Rapior

Thongklang

et al. 2021

JoF

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Macrofungi production and economic value have been increasing globally. The demand for macrofungi has expanded rapidly owing to their popularity among consumers, pleasant taste, and unique flavors. The presence of high quality proteins, polysaccharides, unsaturated fatty acids, minerals, triterpene sterols, and secondary metabolites makes macrofungi an important commodity. Macrofungi are well known for their ability to protect from or cure various health problems, such as immunodeficiency, cancer, inflammation, hypertension, hyperlipidemia, hypercholesterolemia, and obesity. Many studies have demonstrated their medicinal properties, supported by both in vivo and in vitro experimental studies, as well as clinical trials. Numerous bioactive compounds isolated from mushrooms, such as polysaccharides, proteins, fats, phenolic compounds, and vitamins, possess strong bioactivities. Consequently, they can be considered as an important source of nutraceuticals. Numerous edible mushrooms have been studied for their bioactivities, but only a few species have made it to the market. Many species remain to be explored. The converging trends and popularity of eastern herbal medicines, natural/organic food product preference, gut-healthy products, and positive outlook towards sports nutrition are supporting the growth in the medicinal mushroom market. The consumption of medicinal mushrooms as functional food or dietary supplement is expected to markedly increase in the future. The global medicinal mushroom market size is projected to increase by USD 13.88 billion from 2018 to 2022. The global market values of promising bioactive compounds, such as lentinan and lovastatin, are also expected to rise. With such a market growth, mushroom nutraceuticals hold to be very promising in the years to come.

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“…Recently, the cordycepin and Cordyceps-derived polysaccharides have been found to attenuate microbiota dysfunction and improve obesity in rat and mice [17,18]. Many experiments have found that Cordyceps-derived polysaccharides have many physiological regulation potentials, including preventing damage of intestinal cells [19] and promoting immunostimulatory activity [20].…”

Section: Introductionmentioning

confidence: 99%

Polysaccharides Obtained from Cordyceps militaris Alleviate Hyperglycemia by Regulating Gut Microbiota in Mice Fed a High-Fat/Sucrose Diet

Lee

Chen

Hsu

et al. 2021

Foods

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Polysaccharides isolated from fungus Cordyceps militaris display multi-biofunctions, such as immunostimulation, down-regulation of hyperlipidemia, and anti-cancer function. The occurrence of obesity and metabolic syndrome is related to the imbalance of gut microbiota. In this study, the effects of C. militaris and its fractions on modifying metabolic syndrome in mice were evaluated. Mice were fed a high-fat/high-sucrose diet (HFSD) for 14 weeks to induce body weight increase and hyperlipidemia symptoms in mice, and then the mice were simultaneously given a HFSD and C. militaris samples for a further 8 weeks. The results indicated that the fruit body, polysaccharides, and cordycepin obtained from C. militaris had different efficacies on regulating metabolic syndrome and gut microbiota in HFSD-treated mice. Polysaccharides derived from C. militaris decreased the levels of blood sugar and serum lipids in mice fed HFSD. In addition, C. militaris-polysaccharide treatment obviously improved intestinal dysbiosis through promoting the population of next generation probiotic Akkermansia muciniphila in the gut of mice fed HFSD. In conclusion, polysaccharides derived from C. militaris have the potential to act as dietary supplements and health food products for modifying the gut microbiota to improve the metabolic syndrome.

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Polysaccharides isolated from Cordyceps Sinensis contribute to the progression of NASH by modifying the gut microbiota in mice fed a high-fat diet

Cited by 27 publications

References 81 publications

Evaluation on the characteristics of gut microbiome in polycystic ovary syndrome rats induced by dihydrotestosterone or letrozole

Evaluation on the characteristics of gut microbiome in polycystic ovary syndrome rats induced by dihydrotestosterone or letrozole

Macrofungi as a Nutraceutical Source: Promising Bioactive Compounds and Market Value

Polysaccharides Obtained from Cordyceps militaris Alleviate Hyperglycemia by Regulating Gut Microbiota in Mice Fed a High-Fat/Sucrose Diet

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