Polysaccharides from Traditional Chinese Medicines: Extraction, Purification, Modification, and Biological Activity

Chen, Yun; Yao, Fangke; Ke, Ming; Wang, Deyun; Hu, Yuanliang; Liu, Jiaguo

doi:10.3390/molecules21121705

Cited by 154 publications

(88 citation statements)

References 195 publications

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“…Polysaccharides have been recognized as one of the substances used worldwide in food preparation and processing as thickeners, emulsifiers, or stabilizers (Hu, Nie, Li, Wang, & Xie, 2017). Numerous studies have reported that polysaccharides have anticancer, antioxidant, and antibacterial activities (Chen, Yao, et al, 2016;Li, Yuan, Zhou, Zeng, & Lu, 2016;Ou, Yu, Zhang, & Zhu, 2016;Wang, Liu, Huang, & Zhang, 2016). Certain studies have shown that aqueous and ethanol extracts of the rhizome of Osmunda japonica Thunb possess antifungal activity (Zhu et al, 2013).…”

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confidence: 99%

Effect of polysaccharide derived from Osmunda japonica Thunb ‐incorporated carboxymethyl cellulose coatings on preservation of tomatoes

Zhang

Liu

et al. 2019

J Food Process Preserv

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Polysaccharides from Osmunda japonica Thunb (Op) exhibit a wide range of biological activities including antibacterial, anti‐inflammatory, antioxidant, and antitumor effects. Carboxymethyl cellulose (CMC) is a type of cellulose derivative that can form dense films. CMC can slow the respiratory transpiration of tomatoes and reduce water evaporation. Coatings of CMC (0.7% concentration) with various levels of Op (0.02%, 0.04%, 0.06%, 0.08%, and 0.1%) were used to preserve tomatoes at room temperature. The treatment, weight loss, hardness, titratable acidity (TA), respiratory intensity, and concentrations of ascorbic acid, polyphenol oxidase, and malondialdehyde (MDA) were evaluated in tomatoes after 0, 5, 10, 15, 20, and 25 days of storage. Compared with untreated tomatoes, the tomatoes with Op‐incorporated CMC coatings had reduced respiratory intensity, weight loss and ascorbic acid loss, increased TA, controlled MDA, and inhibited the decay of tomatoes. Therefore, the use of Op‐incorporated CMC coatings extended the shelf life and maintained the quality of postharvest tomatoes. Practical applications In the past decades, the use of edible coatings has attracted widespread interest in extending the shelf life of particularly perishable foods. Currently, applications of edible coatings have been proven to be promising as a tool to improve the quality and extend the shelf life of various fruits. But there have been no reports on the effect of Op‐incorporated CMC treatment on the postharvest quality of tomatoes during storage. The purpose of this work is to investigate the efficiency of Op‐incorporated CMC treatment to extend shelf life and provide theoretical guidance for the preservation of tomatoes.

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confidence: 99%

Effect of polysaccharide derived from Osmunda japonica Thunb ‐incorporated carboxymethyl cellulose coatings on preservation of tomatoes

Zhang

Liu

et al. 2019

J Food Process Preserv

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“…В составе растительной клетки присутствуют свободные моносахариды, водорастворимые полисахариды, пектиновые вещества, целлюлозы и гемицеллюлозы. Выделение сахаров из растительного сырья происходит в несколько этапов [8]. Первым шагом является обезжиривание, для этого обычно применяют экстракцию по Сокслету с использованием органического растворителя, такого как петролейный эфир и 95% этанол, этот шаг обязателен для богатых липидами растений.…”

Section: извлечение углеводов из растительного сырьяunclassified

The allocation of carbohydrates from vegetable raw materials and their identification with the use of capillary electrophoresis

2019

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Relevance. Carbohydrates are integral components of the cells and tissues of all living organisms of the plant and animal world; they are important components of the plant cell walls, as well as the extracellular matrix of animal and human tissues. The type of monosaccharide composition of polysaccharides determines their biological functions for the organism. However, it is difficult to establish the relationship between the structure of the carbohydrate molecule and its biological function.Methods. In this article, the author provides an overview of methods for identifying the monosaccharide composition of plant polymers by capillary electrophoresis, in order to optimize the sample preparation procedure and the conditions of analysis. A scheme for the stepwise release of polysaccharides from raw materials is given: the isolation of soluble monosaccharides and polysaccharides, the extraction of the pectin fraction, the extraction of cellulose and hemicelluloses, in order to study the composition of each fraction. A procedure for acid hydrolysis of polymers to monosaccharides using oxidizing agents such as sulfuric and trifluoroacetic acid is described. In the final part of the article, three different schemes for the identification of the monosaccharide composition were analyzed, namely, separation of the components under strongly alkaline conditions, preliminary derivatization with 1-phenyl-3-methyl-5-pyrazolone, preliminary preparation of (S) (-) α-methylbenzylamine S-PEA) derivatives.Results or Findings. An analysis of the literature shows that the composition of polysaccharides is widely studied by various methods. At the same time, a number of problems remain associated with the implementation of these methods in terms of sample preparation and identification of all monosaccharides characteristic of the studied raw materials. The method of capillary electrophoresis can solve some of these problems; however, little has been studied. The study of carbohydrates in plant objects begins with the procedure for extracting carbohydrates (polysaccharides and monosaccharides) from the plant matrix, hydrolysis of polysaccharides, and subsequent identification using the CE method. The optimization of this scheme of the CE method for determining the composition of polysaccharides is an urgent scientific task.

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“…And APS is safe without any distinct toxicity and side effects [9], thus can be used for a long time. However, APS is mainly extracted using water extraction method, which has several disadvantages, such as low active ingredients (polysaccharide content), especially the yield (only about 2.5%) of crude APS [10], and high cost. All of these limit the development and utilization of APS.…”

Section: Introductionmentioning

confidence: 99%

Cellulase-Assisted Extraction, Characterization, and Bioactivity against Rheumatoid Arthritis of Astragalus Polysaccharides

Cao

Wang

et al. 2019

International Journal of Polymer Science

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This study investigated the effect of cellulase on the isolation of crude Astragalus polysaccharide (APS), analyzed the monosaccharide component of deproteinized APS, detected the molecular weights of purified APS, and examined the biological activities and the preliminary mechanism against rheumatoid arthritis (RA). Compared with water extraction method, cellulase-assisted extraction increased the yield of crude APS to 154% and polysaccharide contents to 121%. Crude APS was then purified by ethanol precipitation, Sevag deproteinization, and high-performance liquid chromatography (HPLC) analysis; monosaccharide contents of APS were different after cellulase-assisted method, especially galacturonic acid content which significantly increased. DEAE-52 cellulose column chromatography isolated three polysaccharide fractions, including a neutral polysaccharide (APS-water) and two acidic polysaccharides (APS-NaCl1 and APS-NaCl2). Using high-performance gel permeation chromatography (HPGPC), the molecular weights of APS-water, APS-NaCl1, and APS-NaCl2 were identified as 67.7 kDa, 234.1 kDa, and 189.4 kDa, respectively. Then their therapeutic effects and possible mechanism against RA were explored using type II collagen-induced arthritis (CIA) rat model. APS could significantly reduce paw swelling, serum concentration of IL-1β and TNF-α, and the expression levels of NF-κB-p65 and IκBα in synovial membranes in CIA rats. Our study indicated that cellulase significantly increases the yield and polysaccharide contents of crude APS, improves the product quality, and preserves the biological features against RA in CIA rats.

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Polysaccharides from Traditional Chinese Medicines: Extraction, Purification, Modification, and Biological Activity

Cited by 154 publications

References 195 publications

Effect of polysaccharide derived from Osmunda japonica Thunb ‐incorporated carboxymethyl cellulose coatings on preservation of tomatoes

Effect of polysaccharide derived from Osmunda japonica Thunb ‐incorporated carboxymethyl cellulose coatings on preservation of tomatoes

The allocation of carbohydrates from vegetable raw materials and their identification with the use of capillary electrophoresis

Cellulase-Assisted Extraction, Characterization, and Bioactivity against Rheumatoid Arthritis of Astragalus Polysaccharides

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