Polysaccharides and polyphenols in sea buckthorn leaf tea have synergistic impact on studied colonic strains in vitro and bacteria in vivo

Abstract

Present research on prebiotics focuses on either polysaccharides or polyphenols. This study compared the individual and combined impact of polysaccharide, quercetin, and gallic acid (GA) treatment on three human faecal strains. In vitro pure culturing and correlation analysis confi rmed that the growth of both benefi cial microbe B. longum subsp. longum (0.695, 0.205: R 2 , slope, respectively) and pathogenic C. perfringens (0.712, 0.085: R 2 , slope, respectively) increased due to polysaccharide treatment, and only GA treatment would inhibit C. perfringens (0.789, -0.165: R 2 , slope, respectively) growth. In vivo studies also revealed that genome copies of Bifi dobacterium increased and C. perfringens decreased in the faeces, when a blend of the three nutrients rather than single polysaccharide or polyphenols were fed to rats. These data suggested that combined prebiotic treatment improved human faecal strain composition better than single treatment.

Hippophae rhamnoides L.: A Comprehensive Review on the Botany, Traditional Uses, Phytonutrients, Health Benefits, Quality Markers, and Applications

“…Many studies had shown that H. rhamnoides L. extract and its components such as polyphenols, flavonoids, polysaccharides, and volatile oils had antibacterial effects, as shown in Table . − H. rhamnoides L. had a broad antibacterial spectrum, and had certain inhibitory effects on Gram-positive bacteria, Gram-negative bacteria, and some anaerobic bacteria. In addition, H. rhamnoides L. polysaccharides and polyphenols had synergistic effects, which inhibited the number of Escherichia coli and Clostridium perfringens in the feces of mice, and increased the number of probiotic bifidobacteria, showing a certain role in regulating the intestinal flora …”

“…In addition, H. rhamnoides L. polysacchar-ides and polyphenols had synergistic effects, which inhibited the number of Escherichia coli and Clostridium perf ringens in the feces of mice, and increased the number of probiotic bifidobacteria, showing a certain role in regulating the intestinal flora. 107 Quality Markers Based on Protobotanical Phylogeny. In China, there are 5 species and 8 subspecies of H. rhamnoides L..…”

The ratio of xylooligosaccharide to ferulic acid affects faecal ferulic acid content, short chain fatty acid output, and gut stress

“…The cycle conditions were 30 s at 94 8C, followed by 40 cycles of 5 s at 94 8C and 30 s at 60 8C. The primers for 16S rRNA of Bifidobacterium and Escherichia coli were generated in our previous study (Du et al, 2018). Ten-fold serial dilutions of target stain plasmid were used for the generation of a standard curve.…”