Polysaccharide-mediated green synthesis of silver nanoparticles from Sargassum siliquosum J.G. Agardh: Assessment of toxicity and hepatoprotective activity

Vasquez, Ross D.; Apostol, Jovencio G.; Leon, Joshua D. de; Mariano, Jonathan D.; Mirhan, Chriselle Marie C.; Pangan, Samuel S.; Reyes, Alyssa Gail M.; Zamora, Emmanuel T.

doi:10.1016/j.onano.2016.03.001

Cited by 55 publications

(28 citation statements)

References 33 publications

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“…These findings are in agreement with other results that show zero mortality, absence of abnormal reactions, and minimal effect on kidney and liver indices in male and female Sprague Dawley rats administered orally with 2,000 mg/kg bw of Ag-NPs produced by Sargassum siliquosum, indicating low toxicity of these nanoparticles. 85 Moreover, in the same study, it was observed that a pretreatment with Ag-NPs prevented liver cell damage caused by paracetamol at 100 and 200 mg/kg bw dose. Thus, Ag-NPs obtained by green synthesis provide a cheaper nontoxic method that ensures effectiveness and safety in the application of these nanoparticles in different fields of study.…”

Section: Discussion Synthesis and Characterization Of Ag-npsmentioning

confidence: 80%

In vivo antimicrobial activity of silver nanoparticles produced via a green chemistry synthesis using <em>Acacia rigidula</em> as a reducing and capping agent

Escárcega‐González¹,

Garza-Cervantes²,

Vázquez-Rodríguez³

et al. 2018

IJN

136

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IntroductionOne of the main issues in the medical field and clinical practice is the development of novel and effective treatments against infections caused by antibiotic-resistant bacteria. One avenue that has been approached to develop effective antimicrobials is the use of silver nanoparticles (Ag-NPs), since they have been found to exhibit an efficient and wide spectrum of antimicrobial properties. Among the main drawbacks of using Ag-NPs are their potential cytotoxicity against eukaryotic cells and the latent environmental toxicity of their synthesis methods. Therefore, diverse green synthesis methods, which involve the use of environmentally friendly plant extracts as reductive and capping agents, have become attractive to synthesize Ag-NPs that exhibit antimicrobial effects against resistant bacteria at concentrations below toxicity thresholds for eukaryotic cells.PurposeIn this study, we report a green one-pot synthesis method that uses Acacia rigidula extract as a reducing and capping agent, to produce Ag-NPs with applications as therapeutic agents to treat infections in vivo.Materials and methodsThe Ag-NPs were characterized using transmission electron microscopy (TEM), high-resolution TEM, selected area electron diffraction, energy-dispersive spectroscopy, ultraviolet–visible, and Fourier transform infrared.ResultsWe show that Ag-NPs are spherical with a narrow size distribution. The Ag-NPs show antimicrobial activities in vitro against Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and a clinical multidrug-resistant strain of P. aeruginosa) and Gram-positive (Bacillus subtilis) bacteria. Moreover, antimicrobial effects of the Ag-NPs, against a resistant P. aeruginosa clinical strain, were tested in a murine skin infection model. The results demonstrate that the Ag-NPs reported in this work are capable of eradicating pathogenic resistant bacteria in an infection in vivo. In addition, skin, liver, and kidney damage profiles were monitored in the murine infection model, and the results demonstrate that Ag-NPs can be used safely as therapeutic agents in animal models.ConclusionTogether, these results suggest the potential use of Ag-NPs, synthesized by green chemistry methods, as therapeutic agents against infections caused by resistant and nonresistant strains.

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Section: Discussion Synthesis and Characterization Of Ag-npsmentioning

confidence: 80%

In vivo antimicrobial activity of silver nanoparticles produced via a green chemistry synthesis using <em>Acacia rigidula</em> as a reducing and capping agent

Escárcega‐González¹,

Garza-Cervantes²,

Vázquez-Rodríguez³

et al. 2018

IJN

136

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“…On the other hand, carbohydrate-stabilized nanoparticles usually involve carboxymethyl polysaccharide 29 , 30 and can also be made via biogenic synthesis. 31 , 32 Nevertheless, very few reports have been performed on AgNPs stabilized by nanocellulose. 33 This material can be extracted from various types of biomasses, including oranges, 34 and presents a great potential as a renewable nanomaterial owing to its sustainability, high versatility (in the form of nanofibrils, nanowhiskers, or nanocrystals), and ease of extraction.…”

Section: Introductionmentioning

confidence: 99%

Bio-based synthesis of silver nanoparticles from orange waste: effects of distinct biomolecule coatings on size, morphology, and antimicrobial activity

Barros¹,

Cruz²,

Mayrink³

et al. 2018

NSA

102

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PurposeDespite the numerous reports on biological syntheses of silver nanoparticles (AgNPs), little is known about the composition of their capping agents, protein corona of plant extract-mediated synthesis, and their influence on the properties of AgNPs. Here, orange (Citrus sinensis) waste was utilized as a source of an extract for AgNP synthesis (the protein corona composition of which was elucidated), and also as a starting material for hesperidin and nanocellulose extraction, which were used for bio-based AgNP synthesis. A comparison of the results using the two methods of synthesis is presented.MethodsAgNPs were synthesized using orange (C. sinensis) peel extract (Or-AgNPs) in a biological route, and using hesperidin (Hsd-AgNPs) and nanocellulose (extracted from oranges) in a green chemical route. Characterization of nanoparticles was carried out using zeta potential and hydrodynamic size measurements, transmission electron microscopy, and X-ray diffraction. Elucidation of proteins from protein corona was performed via ultra performance liquid chromatography-tandem mass spectrometer experiments. Antimicrobial activity was assessed via minimum inhibitory concentration assays against Xanthomonas axonopodis pv. citri (Xac), the bacterium that causes citric canker in oranges.ResultsOr-AgNPs were not completely uniform in morphology, having a size of 48.1±20.5 nm and a zeta potential of −19.0±0.4 mV. Stabilization was performed mainly by three proteins, which were identified by tandem mass spectrometry (MS/MS) experiments. Hsd-AgNPs were smaller (25.4±12.5 nm) and had uniform morphology. Nanocellulose provided a strong steric and electrostatic (−28.2±1.0 mV) stabilization to the nanoparticles. Both AgNPs presented roughly the same activity against Xac, with the minimum inhibitory concentration range between 22 and 24 μg mL−1.ConclusionDespite the fact that different capping biomolecules on AgNPs had an influence on morphology, size, and stability of AgNPs, the antibacterial activity against Xac was not sensitive to this parameter. Moreover, three proteins from the protein corona of Or-AgNPs were identified.

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“…Authors concluded that AgNPs synthesized from S. siliquosum have hepatoprotective effects that can keep liver enzymes near to normal in intoxicated groups. However, the toxic effects of AgNPs on the kidney are still not clear and further studies needed to clarify their safety [162].…”

Section: Toxicity Of Polysaccharide-based Agnpsmentioning

confidence: 99%

Green synthesis of polysaccharide-based inorganic nanoparticles and biomedical aspects

Shavandi¹,

Saeedi

Ali

et al. 2019

Functional Polysaccharides for Biomedical Applications

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Polysaccharide-mediated green synthesis of silver nanoparticles from Sargassum siliquosum J.G. Agardh: Assessment of toxicity and hepatoprotective activity

Cited by 55 publications

References 33 publications

In vivo antimicrobial activity of silver nanoparticles produced via a green chemistry synthesis using <em>Acacia rigidula</em> as a reducing and capping agent

In vivo antimicrobial activity of silver nanoparticles produced via a green chemistry synthesis using <em>Acacia rigidula</em> as a reducing and capping agent

Bio-based synthesis of silver nanoparticles from orange waste: effects of distinct biomolecule coatings on size, morphology, and antimicrobial activity

Green synthesis of polysaccharide-based inorganic nanoparticles and biomedical aspects

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