Polysaccharide fraction of Agaricus brasiliensis avoids tumor-induced IL-10 production and changes the microenvironment of subcutaneous Ehrlich adenocarcinoma

Pinto, Andréa Vanessa Ferreira da Silva; Martins, Priscila Raquel; Romagnoli, Graziela Gorete; Campanelli, Ana Paula; Terezan, Ana Paula; Filho, Édson Rodrigues; Eira, Augusto Ferreira da; Kaneno, Ramon

doi:10.1016/j.cellimm.2009.01.002

Cited by 24 publications

(15 citation statements)

References 27 publications

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“…Most research had taken an approach of isolating pharmaceutically active mushroom compounds, especially mushroom polysaccharides, had been reported to exhibit significant anti-tumor activities [30,31]. However, there have been limited studies investigate the protein drugs for oral administration purposes as protein drugs are readily degraded in the low pH environment of the stomach.…”

Section: Discussionmentioning

confidence: 99%

Oral administration of an Enoki mushroom protein FVE activates innate and adaptive immunity and induces anti-tumor activity against murine hepatocellular carcinoma

Chang

Hsieh

Yeh

et al. 2010

International Immunopharmacology

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Section: Discussionmentioning

confidence: 99%

Oral administration of an Enoki mushroom protein FVE activates innate and adaptive immunity and induces anti-tumor activity against murine hepatocellular carcinoma

Chang

Hsieh

Yeh

et al. 2010

International Immunopharmacology

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“…The extracts were co-cultured with effector and target cells for 2 h and 24 h for the cytotoxicity and cytokine production assays, respectively. Cell culture studies of cytokine production typically use 24-to 72-h periods (Masuda et al, 2009;Pinto et al, 2009;Ukawa et al, 2007;Yu et al, 2009). Longer co-culturing periods are thus necessary for the detection of cytokine production than for that of cell cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effect of mushrooms on cytotoxic activity and cytokine production of intestinal lamina propria leukocytes does not necessarily depend on β-glucan contents

et al. 2011

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We evaluated the effects of seven mushroom extracts (Grifola frondosa, Pholiota nameko, Panellus serotinus, Hypsizygus marmoreus, Pleurotus cornucopiae, Armillaria mellea, and Flammulina velutipes) on cytotoxic activity and cytokine production of lamina propria leukocytes (LPLs) isolated from rat small (S) and large (L) intestinal mucosa. Boiling water extracts from seven species of mushrooms showed no direct cytotoxicity against the YAC-1 target cells. However, prominent increases of cytotoxicity were observed in S-and L-LPLs co-cultured with P. serotinus extract. Cytokine production (TNFα, IFNγ, IL-12 p70, and IL-4) of S-and L-LPLs was stimulated in response to P. cornucopiae extract. Mushroom extracts contribute to target cell adhesion and/or cytokine production in the effector cells. The promotion of cytotoxic activity in S-and L-LPLs was not necessarily related to β-glucan content of the mushroom.

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“…Agaricus brasiliensis Wasser et al (formerly Agaricus blazei Murrill) is a mushroom native to Brazil that has a wide range of medicinal properties including antitumor and immunostimulatory activities [1–6]. These activities are mainly attributed to β-glucans found in the polysaccharide fraction of their fruiting bodies [3, 5, 7].…”

Section: Introductionmentioning

confidence: 99%

Agaricus brasiliensis polysaccharides stimulate human monocytes to capture Candida albicans, express toll-like receptors 2 and 4, and produce pro-inflammatory cytokines

Martins

Soares

Domeneghini

et al. 2017

J Venom Anim Toxins Incl Trop Dis

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Background Agaricus brasiliensis is a medicinal mushroom with immunomodulatory and antitumor activities attributed to the β-glucans presented in the polysaccharide fraction of its fruiting body. Since β-glucans enhance cellular immunoresponsiveness, in this study we aimed to evaluate the effect of an acid-treated polysaccharide-rich fraction (ATF) of A. brasiliensis on the ability of human monocytes to adhere/phagocyte C. albicans yeast cells, their expression of pattern recognition receptors and their ability to produce cytokines.MethodsAdhesion/phagocytosis of FITC-labeled C. albicans was evaluated by flow cytometry. Cells were incubated with specific fluorochrome-labeled antibodies for TLR2 and 4, βGR and MR and also evaluated by flow cytometry. Monocytes were cultured with ATF, and culture supernatants were collected for analysis of in vitro cytokine production by ELISA (TNF-α, IL-1β, IL-12 and IL-10).ResultsATF significantly increased the adherence/phagocytosis of C. albicans by monocytes and this was associated with enhanced expression of TLR2 and TLR4, while no effect was observed on βGR or MR. Moreover, expression of TLR4 and TLR2 was associated with higher levels of in vitro production of TNF-α and IL-1, respectively. Production of IL-10 was also increased by ATF treatment, but we found no association between its production and the expression of Toll-like receptors.ConclusionOur results provided us with evidence that A. brasiliensis polysaccharides affect human monocytes probably through the modulation of Toll-like receptors.

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Polysaccharide fraction of Agaricus brasiliensis avoids tumor-induced IL-10 production and changes the microenvironment of subcutaneous Ehrlich adenocarcinoma

Cited by 24 publications

References 27 publications

Oral administration of an Enoki mushroom protein FVE activates innate and adaptive immunity and induces anti-tumor activity against murine hepatocellular carcinoma

Oral administration of an Enoki mushroom protein FVE activates innate and adaptive immunity and induces anti-tumor activity against murine hepatocellular carcinoma

Immunomodulatory effect of mushrooms on cytotoxic activity and cytokine production of intestinal lamina propria leukocytes does not necessarily depend on β-glucan contents

Agaricus brasiliensis polysaccharides stimulate human monocytes to capture Candida albicans, express toll-like receptors 2 and 4, and produce pro-inflammatory cytokines

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