Polysaccharide-Derived Ice Recrystallization Inhibitors with a Modular Design: The Case of Dextran-Based Graft Polymers

Wu, Xiaojun; Qiu, Yuwei; Chen, Changhong; Gao, Yi Qin; Wang, Yanlin; Yao, Fanglian; Zhang, Hong; Li, Junjie

doi:10.1021/acs.langmuir.2c02032

Cited by 3 publications

(4 citation statements)

References 71 publications

(125 reference statements)

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“…Poly(vinyl alcohol) (PVA) exhibits very high IRI activity and is often used as a positive standard for IRI activity. The molecular weight, degree of deacetylation, and tacticity of PVA affect the IRI activity. , Grafted PVAs to hyaluronic acid, pectin, and alginate maintain the IRI activity . Poly(Fulvo-co-Oxo), a facially amphipathic polymer, exhibits high IRI activity .…”

Section: Introductionmentioning

confidence: 99%

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Oligonucleotides as Inhibitors of Ice Recrystallization

Kim

Park

et al. 2023

Biomacromolecules

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Oligonucleotides of adenine (A20), guanine (G20), cytosine (C20), thymine (T20), cytosine–guanine ((CG)20), and adenine–thymine ((AT)20) were investigated as model compounds for ice recrystallization inhibition (IRI). Dehydroxy uracil (dU20), U20, and T20 were also compared to investigate the effect of minute changes in the hydrophobicity of the oligonucleotides on the IRI activity. Among the oligonucleotides considered in this study, T20 exhibited the best performance for IRI. In addition, the degree of polymerization of oligothymines varied over 5, 10, 20, 30, 50, and 100, and T20 was found to be the most effective for IRI. The IRI mechanism was investigated by comparing U20 and T20, which exhibited the lowest and highest IRI activity, respectively, among the oligonucleotides for their dynamic ice-shaping, thermal hysteresis, and ice nucleation inhibition. Little or no dynamic ice-shaping activity and small thermal hysteresis were observed for both nucleotides. All of the findings suggest that not the ice–polymer adhesion but the hydrophobic interactions of T20 in the interface layer might interfere with the water deposition on the ice crystal surfaces and contribute to the IRI activity of the T20 oligonucleotide.

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Section: Introductionmentioning

confidence: 99%

“…13,14 Grafted PVAs to hyaluronic acid, pectin, and alginate maintain the IRI activity. 15 Poly(Fulvo-co-Oxo), a facially amphipathic polymer, exhibits high IRI activity. 16 Recently, poly(Ala-co-Lys) was reported to exhibit very high IRI activity, comparable to that of PVA.…”

Section: ■ Introductionmentioning

confidence: 99%

Oligonucleotides as Inhibitors of Ice Recrystallization

Kim

Park

et al. 2023

Biomacromolecules

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“…In previous studies, we have identified some novel antifreeze agents and discussed corresponding mechanism. − Herein, we choose histidine and phenylalanine as two model molecules to investigate their IRI activity through experiment and molecular dynamics (MD) simulation. Both are α-amino acids containing an alanine moiety and one aromatic ring.…”

Section: Introductionmentioning

confidence: 99%

Perturbation of Hydrogen Bonding Network Plays an Important Role in Determining Antifreeze Activity

Chen

Liu

et al. 2023

J. Phys. Chem. C

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In order to develop new antifreeze agents, it is crucial to understand the antifreeze activity. The adsorption-inhibition mechanism has been widely accepted, and most previous studies focus on icebinding affinity of additive molecules but rarely discuss their influence on the hydrogen bonding network of ice. Herein, we investigate the ice recrystallization inhibition (IRI) activity of two structurally similar molecules, histidine (His) and phenylalanine (Phe). It is verified by experiment that Phe has excellent IRI activity, while His does not. Molecular dynamics simulation is conducted to study the process of ice growth with amino acids and to explore the origin of the difference between His and Phe. We find that His is prone to be swallowed up by ice and thus no longer hinders its growth, whereas Phe can remain unswallowed on the ice surface for a long period, which is attributed to their different abilities to perturb the hydrogen bonding network. Based on a statistical analysis of oxygen−hydrogen bond orientations, we propose a method to evaluate the order of the hydrogen bonding network. With this method, the IRI activity of His and Phe can be well interpreted, and the influence of different groups on the hydrogen bonding network can be quantified. This study takes the structural integrity of ice into consideration and provides a possibility to investigate the influence of different additive molecules on hydrogen bonding network, which is expected to improve the understanding of antifreeze mechanism.

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“…The scaled-up production of AFPs with a reasonable cost is still difficult. Synthetic polymers have been widely developed as AFP biomimetics, such as poly(vinyl alcohol) (PVA), − poly(ethylene glycol) (PEG), and their block copolymers. , Besides, pronounced IRI activities have also been seen on graphene oxide (GO), nanocelluloses with amphiphilic structures, and facially amphipathic glycopolymers synthesized by ring-opening metathesis polymerization, analogous to natural antifreezing glycoprotein such as poly([( l -Ala) x - co -( l -Thr succinate) y , nanogels, high molecular weight polyproline, oligonucleotides, and surface-functionalized MOF particles . Since synthetic materials are less active than natural AFPs, researchers are turning their focus to the higher-ordered nanostructures .…”

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confidence: 99%

The Valence-Dependent Activity of Colloidal Molecules as Ice Recrystallization Inhibitors

Tian,

Xu,

Qiu

et al. 2024

ACS Macro Lett.

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Inspired by advances in cryopreservation techniques, which are essential for modern biomedical applications, there is a special interest in the ice recrystallization inhibition (IRI) of the antifreeze protein (AFPs) mimics. There are in-depth studies on synthetic materials mimicking AFPs, from simple molecular structure levels to complex self-assemblies. Herein, we report the valence-dependent IRI activity of colloidal organic molecules (CMs). The CMs were prepared through polymerization-induced particle-assembly (PIPA) of the ABC-type triblock terpolymer of poly(acryloxyethyl trimethylammonium chloride)-b-poly(benzyl acrylate)-b-poly(diacetone acrylamide) (PATAC-b-PBzA-b-PDAAM) at high monomer conversions. Stabilized by the cationic block of PATAC, the strong intermolecular H-bonding and incompatibility of the PDAAM block with PBzA contributed to the in situ formation of Janus particles (AX1) beyond the initial spherical seed particles (AX0), as well as the high valency clusters of linear AX2 and trigonal AX3. Their distribution was controlled mainly by the polymerization degrees (DPs) of PATAC and PDAAM blocks. IRI activity results of the CMs suggest that the higher fraction of AX1 results in the better IRI activity. Increasing the fraction of AX1 from 27% to 65% led to a decrease of the mean grain size from 39.8% to 10.9% and a depressed growth rate of ice crystals by 58%. Moreover, by replacing the PDAAM block with the temperature-responsive one of poly(N-isopropylacrylamide) (PNIPAM), temperature-adjustable IRI activity was observed, which is well related to the reversible transition of AX0 to AX1, providing a new idea for the molecular design of amphiphilic polymer nanoparticle-based IRI activity materials.

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Polysaccharide-Derived Ice Recrystallization Inhibitors with a Modular Design: The Case of Dextran-Based Graft Polymers

Cited by 3 publications

References 71 publications

Oligonucleotides as Inhibitors of Ice Recrystallization

Oligonucleotides as Inhibitors of Ice Recrystallization

Perturbation of Hydrogen Bonding Network Plays an Important Role in Determining Antifreeze Activity

The Valence-Dependent Activity of Colloidal Molecules as Ice Recrystallization Inhibitors

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