Polysaccharide-based strategies for heart tissue engineering

Silva, Amanda; Juenet, Maya; Meddahi‐Pellé, Anne; Letourneur, Didier

doi:10.1016/j.carbpol.2014.06.010

Cited by 55 publications

(38 citation statements)

References 119 publications

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“…37, 21 Without Myf5 and MyoD, myogenic cells will fail to progress normally during the determination stage of myogenesis. 50, 51, 15, 46 Semi-quantitative analysis of the specimens confirmed that engrafted GMSCs expressed higher amounts of myogenic related genes (MF20, Myf5, and MyoD) than engrafted hBMMSCs (P-value < 0.05) (Fig. 3b).…”

Section: Resultsmentioning

confidence: 82%

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Muscle Tissue Engineering Using Gingival Mesenchymal Stem Cells Encapsulated in Alginate Hydrogels Containing Multiple Growth Factors

Ansari

Chen

et al. 2016

Ann Biomed Eng

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Repair and regeneration of muscle tissue following traumatic injuries or muscle diseases often presents a challenging clinical situation. If a significant amount of tissue is lost the native regenerative potential of skeletal muscle will not be able to grow to fill the defect site completely. Dental-derived mesenchymal stem cells (MSCs) in combination with appropriate scaffold material, present an advantageous alternative therapeutic option for muscle tissue engineering in comparison to current treatment modalities available. To date, there has been no report on application of gingival mesenchymal stem cells (GMSCs) in three-dimensional scaffolds for muscle tissue engineering. The objectives of the current study were to develop an injectable 3D RGD-coupled alginate scaffold with multiple growth factor delivery capacity for encapsulating GMSCs, and to evaluate the capacity of encapsulated GMSCs to differentiate into myogenic tissue in vitro and in vivo where encapsulated GMSCs were transplanted subcutaneously into immunocompromised mice. The results demonstrate that after 4 weeks of differentiation in vitro, GMSCs as well as the positive control human bone marrow mesenchymal stem cells (hBMMSCs) exhibited muscle cell-like morphology with high levels of mRNA expression for gene markers related to muscle regeneration (MyoD, Myf5, and MyoG) via qPCR measurement. Our quantitative PCR analyses revealed that the stiffness of the RGD-coupled alginate regulates the myogenic differentiation of encapsulated GMSCs. Histological and immunohistochemical/fluorescence staining for protein markers specific for myogenic tissue confirmed muscle regeneration in subcutaneous transplantation in our in vivo animal model. GMSCs showed significantly greater capacity for myogenic regeneration in comparison to hBMMSCs (P<0.05). Altogether, our findings confirmed that GMSCs encapsulated in RGD-modified alginate hydrogel with multiple growth factor delivery capacity is a promising candidate for muscle tissue engineering.

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Section: Resultsmentioning

confidence: 82%

“…3a). 29, 46 MyoD is one of the key regulators of muscle differentiation. 37 Myf5 is another protein member of this family It is a protein with a key role in regulating muscle differentiation or myogenesis.…”

Section: Resultsmentioning

confidence: 99%

Muscle Tissue Engineering Using Gingival Mesenchymal Stem Cells Encapsulated in Alginate Hydrogels Containing Multiple Growth Factors

Ansari

Chen

et al. 2016

Ann Biomed Eng

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“…Bernanke and Markwald showed that hyaluronan treatment of avian AV cushion explants resulted in an increase of mesenchymal cells invading a 3D collagen matrix [103]. Large hyaluronan molecules are space-illing polymers presenting regulatory as well as structural functions, while small hyaluronan fragments are involved in immune stimulation, angiogenesis and inlammation [104]. 2-iminothiolane-grafted hyaluronan hydrogel and sodium periodate oxidated hyaluronan cross-linked with adipic acid dihydrazide hydrogel were implanted into rat adductor muscles.…”

Section: Lim Et Al Related That Their Results On Poly(l-lactic Acid)mentioning

confidence: 99%

Three-Dimensional and Biomimetic Technology in Cardiac Injury After Myocardial Infarction: Effect of Acellular Devices on Ventricular Function and Cardiac Remodelling

Chaud¹,

Alves²,

Rebelo³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Dilated cardiomyopathy (DMC) of ischemic or non-ischemic aetiology remains a lethal condition nowadays. Despite early percutaneous or medical revascularization after an acute myocardial infarct (AMI), many patients still develop DMC and severe heart failure due to cardiac remodelling. Possibility of regenerating myocardium already damaged or at least inducing a more positive cardiac remodelling with use of biodegradable scafolds has been atempted in many experimental studies, which can be cellular or acellular. In the cellular scafolds, the cells are incorporated in the structure prior to implantation of the same into the injured tissue. Acellular scafolds, in turn, are composites that use one or more biomaterials present in the extracellular matrix (ECM), such as proteoglycans non-proteoglycan polysaccharide, proteins and glycoproteins to stimulate the chemotaxis of cellular/molecular complexes as growth factors to initiate speciic regeneration. For the development of scafold, the choice of biomaterials to be used must meet speciic biological, chemical and architectural requirements like ECM of the tissue of interest. In acute myocardial infarction, treating the root of the problem by repairing injured tissue is more beneicial to the patient. Inducing more constructive forms of endogenous repair. Thus, patches of acellular scafolds capable of mimicking the epicardium and ECM should be able to atenuate both cardiac remodelling and adverse cardiac dysfunction.

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“…Polysaccharide‐based materials have good biocompatibility with acceptable host response and have the ability to promote cell adhesion, proliferation, and differentiation . Considering the structural and biological functions of polysaccharides, they have raised interest for tissue engineering such as skin, cartilage, heart, and liver.…”

Section: Biomaterials In Liver Regenerationmentioning

confidence: 99%

Advanced Biomaterials and Processing Methods for Liver Regeneration: State‐of‐the‐Art and Future Trends

Morais

Vieira

Zhao

et al. 2020

Adv Healthcare Materials

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Liver diseases contribute markedly to the global burden of mortality and disease. The limited organ disposal for orthotopic liver transplantation results in a continuing need for alternative strategies. Over the past years, important progress has been made in the field of tissue engineering (TE). Many of the early trials to improve the development of an engineered tissue construct are based on seeding cells onto biomaterial scaffolds. Nowadays, several TE approaches have been developed and are applied to one vital organ: the liver. Essential elements must be considered in liver TE—cells and culturing systems, bioactive agents or growth factors (GF), and biomaterials and processing methods. The potential of hepatocytes, mesenchymal stem cells, and others as cell sources is demonstrated. They need engineered biomaterial‐based scaffolds with perfect biocompatibility and bioactivity to support cell proliferation and hepatic differentiation as well as allowing extracellular matrix deposition and vascularization. Moreover, they require a microenvironment provided using conventional or advanced processing technologies in order to supply oxygen, nutrients, and GF. Herein the biomaterials and the conventional and advanced processing technologies, including cell‐sheets process, 3D bioprinting, and microfluidic systems, as well as the future trends in these major fields are discussed.

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Polysaccharide-based strategies for heart tissue engineering

Cited by 55 publications

References 119 publications

Muscle Tissue Engineering Using Gingival Mesenchymal Stem Cells Encapsulated in Alginate Hydrogels Containing Multiple Growth Factors

Muscle Tissue Engineering Using Gingival Mesenchymal Stem Cells Encapsulated in Alginate Hydrogels Containing Multiple Growth Factors

Three-Dimensional and Biomimetic Technology in Cardiac Injury After Myocardial Infarction: Effect of Acellular Devices on Ventricular Function and Cardiac Remodelling

Advanced Biomaterials and Processing Methods for Liver Regeneration: State‐of‐the‐Art and Future Trends

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