Polysaccharide-based polyelectrolyte multilayers

Crouzier, Thomas; Boudou, Thomas; Picart, Catherine

doi:10.1016/j.cocis.2010.05.007

Cited by 175 publications

(132 citation statements)

References 99 publications

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“…59 Recently, polysaccharide-based LbL films have attracted attention in the development of biosensors and drug-delivery systems because of their biocompatibility and high swellability. 60 Polysaccharide LbL films containing enzymes have been prepared for biosensors and bioreactors. [61][62][63] We have studied the voltammetric response of a redox species on Au electrodes coated with polysaccharide LbL films.…”

Section: ·1 Electrostatic Bonded Lbl Filmsmentioning

confidence: 99%

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012

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We present an overview of the preparation and properties of layer-by-layer (LbL) deposited thin films and microcapsules in relation to their use in the development of biosensors and controlled release systems. Enzyme biosensors can be constructed by immobilizing enzymes on the surface of electrodes by LbL deposition without loss of their catalytic activity. In addition to synthetic polymers, binding proteins, such as avidin and lectin, are also used for constructing LbL films through avidin-biotin and lectin-sugar interactions. The performance characteristics of LbL film-based biosensors can be tuned by controlling the number of layers and by the choice of film components. The permeability of polyelectrolyte LbL films to ions and molecules is discussed in relation to the use of the films for eliminating interference in biosensors. The possible use of polysaccharide LbL film-coated electrodes for the construction of biosensors is highlighted, and examples of LbL film-and microcapsule-based optical sensors are described. We then focus on the use of LbL films and microcapsules as vehicles for controlled release, in particular on recent progress in the controlled release of insulin from LbL films and microcapsules. LbL films composed of insulin and polymers are sensitive to environmental pH, and release insulin in response to pH changes, suggesting that insulin LbL films can be used in the development of orally administered insulin. The construction of glucose-dependent insulin release systems using LbL insulin microcapsules functionalized with phenylboronic acid, lectin, and glucose oxidase is also examined.

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Section: ·1 Electrostatic Bonded Lbl Filmsmentioning

confidence: 99%

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012

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“…Layer-by-layer (LbL)-deposited multilayer assemblies have been prepared using a variety of polymeric materials [1][2][3][4] for the development of thin film devices [5][6][7][8][9]. Recently, glucose-sensitive multilayer assemblies have attracted much attention because of their potential use for glucose sensors and drug delivery systems [10][11][12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Preparation of multilayer films consisting of glucose oxidase and poly(amidoamine) dendrimer and their stability

2015

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Multilayer films comprising of glucose oxidase (GOx) and phenylboronic acid-modified poly(amidoamine) dendrimer (PBA-PAMAM) were prepared and their pHand glucose-induced decomposition was studied. Multilayered GOx/PBA-PAMAM films were prepared through boronate ester linkages between PBA-PAMAM and carbohydrate chains of GOx. In contrast, preparation of multilayer film was unsuccessful when unmodified PAMAM was used in lieu of PBA-PAMAM, confirming an essential role of PBA residues in constructing multilayer films. The ten-layered (GOx/PBA-PAMAM) 10 film was stable in aqueous solutions of pH 8.5, whereas the multilayer film decomposed in neutral and acidic media as a result of cleavage of the boronate ester bond. The pH threshold for the film decomposition was pH 7.0-8.0. In addition, the (GOx/PBA-PAMAM) 10 film decomposed in glucose solutions owing to the oxidative scission of carbon-boron bond of PBA-PAMAM by enzymatically produced hydrogen peroxide. Thus, the potential use of the (GOx/PBA-PAMAM) 10 film for the development of pH-and glucosesensitive devices is suggested.

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“…Due to the extreme variations in height and width seen in the peak-like microstructures, the thickness of the films was never measured. Hydration and swelling also play a large role in the final thickness of a film, with large differences between dry and wet films [45]. Hydrated AFM was attempted, but was never successful due to the soft nature of the films (results not shown).…”

Section: Physical Characterization Of Chitosan (Chi)/hyaluronan (Ha)/mentioning

confidence: 99%

Development of a Biocompatible Layer-by-Layer Film System Using Aptamer Technology for Smart Material Applications

Foster

DeRosa

2014

Polymers

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Aptamers are short, single-stranded nucleic acids that fold into well-defined three dimensional (3D) structures that allow for binding to a target molecule with affinities and specificities that can rival or in some cases exceed those of antibodies. The compatibility of aptamers with nanostructures such as thin films, in combination with their affinity, selectivity, and conformational changes upon target interaction, could set the foundation for the development of novel smart materials. In this study, the development of a biocompatible aptamer-polyelectrolyte film system was investigated using a layer-by-layer approach. Using fluorescence microscopy, we demonstrated the ability of the sulforhodamine B aptamer to bind its cognate target while sequestered in a chitosan-hyaluronan film matrix. Studies using Ultraviolet-visible (UV-Vis) spectrophotometry also suggest that deposition conditions such as rinsing time and volume play a strong role in the internal film interactions and growth mechanisms of chitosan-hyaluronan films. The continued study and development of aptamer-functionalized thin films provides endless new opportunities for novel smart materials and has the potential to revolutionize the field of controlled release.

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Polysaccharide-based polyelectrolyte multilayers

Cited by 175 publications

References 99 publications

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

Preparation of multilayer films consisting of glucose oxidase and poly(amidoamine) dendrimer and their stability

Development of a Biocompatible Layer-by-Layer Film System Using Aptamer Technology for Smart Material Applications

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