Polyribosome dissociation and formation in intact reticulocytes with conservation of messenger ribonucleic acid.

Marks, Paul A.; Burka, Edward R.; Conconi, F; Perl, William; Rifkind, Richard A.

doi:10.1073/pnas.53.6.1437

Cited by 85 publications

(22 citation statements)

References 22 publications

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“…If this is true, inhibition of pyrogen production by sodium fluoride added prior to 2 hr raises the possibility that in mature human neutrophils and monocytes, the energy provided by anaerobic glycolysis might be essential for induction of this genome, after which the cells are then capable of utilizing stored energy both for m-RNA transcription and translation to new protein. An alternative explanation is derived from studies on rabbit reticulocytes in which addition of sodium fluoride caused a reversible degradation of polysomes into single ribosomes, thereby halting protein synthesis (40). Human leukocytes may be similar in that glycolytic energy is required for the integrity of poly-somes during protein synthesis.…”

Section: Discussionmentioning

confidence: 99%

Studies on the Origin of Human Leukocytic Pyrogen

Nordlund

Root

Wolff

1970

The Journal of Experimental Medicine

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Granulocytes and monocytes derived from inflammatory exudates or the peripheral circulation of several mammalian species (1-5) can be stimulated to release a pyrogen, leukocytic pyrogen (LP) 1, which induces a characteristic febrile response in rabbits. Chemical characterization of LP has shown it to be a protein molecule (6-8) and since the original discovery of its production by rabbit peritoneal exudate cells (9), many attempts have been made to determine the mechanism of its synthesis by leukocytes. With one exception (10), preformed pyrogen has not been obtained from disrupted rabbit cells (11,12). Its release in vitro has been shown to take place over a several hour period (4, 11, 12), to be dependent upon the temperature of incubation (11), and inhibited by agents which bind sulfhydryl groups in enzymes (13). These findings suggest that LP is either synthesized de novo or activated from a precursor substance after contact with an appropriate stimulating agent.In the present studies, the role of RNA and protein synthesis and the energy requirements for pyrogen release by human peripheral leukocytes activated by the ingestion of heat-killed bacteria have been investigated. The results reported here indicate that both transcription of messenger RNA and its translation by ribosomes into

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Section: Discussionmentioning

confidence: 99%

Studies on the Origin of Human Leukocytic Pyrogen

Nordlund

Root

Wolff

1970

The Journal of Experimental Medicine

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“…Thus, the rate of synthesis is controlled by the rate of formation of messenger RNA. Regulation of protein synthesis at the translation level has been suggested, however, and several reports provide evidence of alterations in polysome activities in the absence of a concomitant messenger RNA synthesis (5,7,11).…”

Section: Introductionmentioning

confidence: 99%

Evidence for Regulation of Protein Synthesis at the Translation Level in Response to Dietary Alterations

Decken

1967

The Journal of Cell Biology

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Rats were maintained for several days on a protein-free diet which, approximately 14 hr before decapitation, was changed to a protein-rich dict. Microsomal subfractions of liver were obtained by sucrose densi W gradient centrifugation. The sedimentation patterns of the microsomal subfractions and of the polysomes were found to be unchanged after the alteration of the diet whereas the ability to incorporate amino acid was markedly enhanced. No significant differences in the RNA/protein ratio of the microsomal subfractions were observed. The changes in amino acid incorporation which were unrelated to concurrent changes in the amount of polysomes are discussed.

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“…KCN is an inhibitor of cell respiration which acts by inhibiting cyto chrome a. Protein synthesis is also inhibited by these agents [13]. NaF was a potent inhibitor of MITC.…”

Section: Resultsmentioning

confidence: 99%

Mechanism of BCG-Activated Macrophage-Induced Tumor Cell Cytotoxicity: Evidence for Both Oxygen-Dependent and Independent Mechanisms

DiStefano

Beck

Zucker

1983

Int Arch Allergy Immunol

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Activated peritoneal macrophages have been shown to be cytotoxic to cancer cells. Bacillus Calmette-Guerin (BCG)-activated rat peritoneal macrophages have a basal cytolytic potential for ³H-thymidine-labelled Walker 256 cancer cells in vitro that can be markedly enhanced by digitonin. This stimulation of cytotoxicity can be partially inhibited by catalase and the combination of superoxide dismutase plus catalase. This suggests that digitonin stimulates activated macrophages to produce superoxide, hydrogen peroxide and possibly other free radicals which can augment macrophage-induced tumor cell cytotoxicity. After a 2-hour incubation with digitonin, macrophages are no longer stimulated by digitonin. However, after a 2-hour drug preincubation period, inhibitors of serine protease activity (DFP, TLCK, SBTI) and inhibitors of protein synthesis (cycloheximide) are potent inhibitors of basal macrophage-induced tumor cytotoxicity. We suggest that BCG-activated macrophages have two mechanisms for destroying cancer cells: one mediated by proteolytic activity, and a second mechanism dependent on the generation of oxygen-derived free radicals.

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Polyribosome dissociation and formation in intact reticulocytes with conservation of messenger ribonucleic acid.

Cited by 85 publications

References 22 publications

Studies on the Origin of Human Leukocytic Pyrogen

Studies on the Origin of Human Leukocytic Pyrogen

Evidence for Regulation of Protein Synthesis at the Translation Level in Response to Dietary Alterations

Mechanism of BCG-Activated Macrophage-Induced Tumor Cell Cytotoxicity: Evidence for Both Oxygen-Dependent and Independent Mechanisms

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