PolyQ-mediated regulation of mRNA granules assembly

Castilla-Llorente, Virginia; Ramos, Andrés

doi:10.1042/bst20140099

Cited by 7 publications

(11 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such repeats are present in many regulatory RNA-binding proteins (68), including Bicoid and GIGYF2. These and other low-complexity regions have been implicated in protein aggregation, and in formation of hydrogels and RNA-protein granules (81). EIF4E1 is found in starvation granules (82) (http://tryptag.org/?query=EIF4E1), but it is distributed throughout the cytosol and nucleus under normal conditions (55).…”

Section: Discussionmentioning

confidence: 99%

The suppressive cap-binding-complex factor 4EIP is required for normal differentiation

Clayton

Terrao

Marucha

et al. 2018

Preprint

View full text Add to dashboard Cite

Summary/AbstractTrypanosoma brucei live in mammals as bloodstream forms and in the Tsetse midgut as procyclic forms. Differentiation from one form to the other proceeds via a growth-arrested stumpy form with low mRNA content and translation. The parasites have six eIF4Es and five eIF4Gs. EIF4E1 pairs with the mRNA-binding protein 4EIP but not with any EIF4G. EIF4E1 and 4EIP each inhibit expression when tethered to a reporter mRNA. The 4E-binding motif in 4EIP is required for the interaction with EIF4E1 both in vivo and in a 2-hybrid assay, but not for the suppressive activity of 4EIP when tethered. However, the suppressive activity of EIF4E1 when tethered requires 4EIP. Correspondingly, in growing bloodstream forms, 4EIP is preferentially associated with unstable mRNAs. Trypanosomes lacking 4EIP have a marginal growth disadvantage as cultured bloodstream or procyclic forms. Bloodstream forms without 4EIP cannot make differentiation-competent stumpy forms, but the defect can be complemented by a truncated 4EIP that does not interact with EIF4E1. Bloodstream forms lacking EIF4E1 have a growth defect but can differentiate. We suggest that 4EIP and EIF4E1 fine-tune mRNA levels in growing cells, and that 4EIP is required for mRNA suppression during differentiation to the stumpy form.

show abstract

Section: Discussionmentioning

confidence: 99%

The suppressive cap-binding-complex factor 4EIP is required for normal differentiation

Clayton

Terrao

Marucha

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Interestingly, polyQs were also found to be enriched in TFs in plants 26 . Besides that, polyQ-containing proteins are proposed to act as key factors for the formation of RNA granules, which are ribonucleoprotein particles that mediate mRNA compartmentalisation 27 . Generally, the dynamic formation of subcellular structures could be necessary for a changing composition of the assemblies in dependence of their functional status 23 .…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, we found that PLT3, in contrast to PLT1 and PLT2, has polyQ containing PrDs in its aa sequence that are necessary for the localisation to NBs and for complex-formation with WOX5. Proteins containing polyQ-stretches or PrDs are often involved in RNA binding, RNA processing and/or RNA compartmentalisation 27,35,38–41 and indeed, the PLT3 NBs co-localise with RNA. Just as PLT3, FLOWERING CONTROL LOCUS A (FCA) is a PrD-containing protein 30 that localises to subnuclear structures 41 .…”

Section: Discussionmentioning

confidence: 99%

PLETHORA-WOX5 interaction and subnuclear localisation controlArabidopsisroot stem cell maintenance

Burkart

Strotmann

Kirschner

et al. 2019

Preprint

View full text Add to dashboard Cite

Total word count: 7260 20 of the PLT-WOX5 complexes to NBs, possibly by liquid-liquid phase separation, plays an 38 important role during determination of CSC fate. 39 differentiation 6,7 . WOX5 also represses QC divisions, maintaining the quiescence of the QC by 65 repressing CYCLIN D (CYCD) activity within the QC 8 . The auxin-induced PLTs form a clade 66 of six TFs, and act as master regulators of root development, as multiple plt mutants fail to 67 develop functional RAMs 5,9,10 . PLT1, 2, 3 and 4 are expressed mainly in and around the QC 68 and form an instructive gradient, which is required for maintaining the balance of stem cell fate 69 and differentiation. This PLT gradient is also necessary for separating auxin responses in the 70 SCN and for the correct positioning of the QC and the expression of QC markers 5,9,10 . 71Genetically, WOX5 and PLT1 were shown to play an interconnected role in auxin-regulated 72 CSC fate, whereas PLT1 and PLT3 were found to positively regulate WOX5 expression 11,12 . 73 Although the implication of PLTs and WOX5 in controlling stem cell regulation and 74 maintenance in the Arabidopsis RAM is well established and genetic evidence for cross 75 regulation exists, the underlying molecular mechanisms remain largely elusive. Here, we show 76 that the mutual regulation of expression, but also the ability of PLTs, especially PLT3, to recruit 77 WOX5 to NBs in CSCs controls stem cell homeostasis in the Arabidopsis RAM. We propose 78 a model in which the differential PLT/WOX5 complexes depending on subnuclear localisation 79 regulate stem cell fate in the RAM, possibly by phase separation of PLT3 to NBs. 80Suppl. Table 7). In the plt2 and plt3 single mutants, the frequency of roots lacking a CSC layer 130 increases to above 30 % (36 % and 32 %, respectively), and in the plt2, plt3 double mutant to 131 41% (see Fig. 3c,d,e,i, Suppl. Table 7). Interestingly, the wox5, plt3 double mutant as well as 132 the wox5, plt2, plt3 triple mutant show a frequency of differentiated CSCs comparable to the 133 wox5 single mutant (71 % and 77 %, respectively) ( Fig. 3g,h,i, Suppl. Table 7). This data 134suggests that PLTs and WOX5 may act together in the same pathway to maintain CSC 135 homeostasis, as there is no additive effect observable in the multiple mutant roots. To analyse 136

show abstract

“…Such repeats are present in many regulatory RNA-binding proteins, both from trypanosomes ( 68 ), and other species (for example, they are found in Bicoid and GIGYF2). These and other low complexity regions have been implicated in protein aggregation and in formation of hydrogels and RNA–protein granules ( 85 ). EIF4E1 is found in starvation granules ( 78 ) ( http://tryptag.org/?query=EIF4E1 ), but it is distributed throughout the cytosol and nucleus under normal conditions ( 55 ).…”

Section: Discussionmentioning

confidence: 99%

The suppressive cap-binding complex factor 4EIP is required for normal differentiation

Terrao

Marucha

Mugo

et al. 2018

Nucleic Acids Research

View full text Add to dashboard Cite

Trypanosoma brucei live in mammals as bloodstream forms and in the Tsetse midgut as procyclic forms. Differentiation from one form to the other proceeds via a growth-arrested stumpy form with low messenger RNA (mRNA) content and translation. The parasites have six eIF4Es and five eIF4Gs. EIF4E1 pairs with the mRNA-binding protein 4EIP but not with any EIF4G. EIF4E1 and 4EIP each inhibit expression when tethered to a reporter mRNA, but while tethered EIF4E1 suppresses only when 4EIP is present, suppression by tethered 4EIP does not require the interaction with EIF4E1. In growing bloodstream forms, 4EIP is preferentially associated with unstable mRNAs. Bloodstream- or procyclic-form trypanosomes lacking 4EIP have only a marginal growth disadvantage. Bloodstream forms without 4EIP are, however, defective in translation suppression during stumpy-form differentiation and cannot subsequently convert to growing procyclic forms. Intriguingly, the differentiation defect can be complemented by a truncated 4EIP that does not interact with EIF4E1. In contrast, bloodstream forms lacking EIF4E1 have a growth defect, stumpy formation seems normal, but they appear unable to grow as procyclic forms. We suggest that 4EIP and EIF4E1 fine-tune mRNA levels in growing cells, and that 4EIP contributes to translation suppression during differentiation to the stumpy form.

show abstract

PolyQ-mediated regulation of mRNA granules assembly

Cited by 7 publications

References 43 publications

The suppressive cap-binding-complex factor 4EIP is required for normal differentiation

The suppressive cap-binding-complex factor 4EIP is required for normal differentiation

PLETHORA-WOX5 interaction and subnuclear localisation controlArabidopsisroot stem cell maintenance

The suppressive cap-binding complex factor 4EIP is required for normal differentiation

Contact Info

Product

Resources

About