Polyploidy Induction of Moth Orchid (Phalaenopsis amabilis (L.) Blume) by Colchicine Treatment on Pollinated Flowers

Azmi, Tubagus Kiki Kawakibi; Sukma, Dewi; Aziz, Sandra Arifin; Syukur, Muhamad

doi:10.4038/jas.v11i2.8118

Cited by 12 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is effective to induce polyploidy in tissues that are obtained from those organs which have highly morphogenetic responses such as embryo, apical meristems and young inflorescence [24]. Although colchicine is readily used to induce polyploidy in many crops, there are still limitations in developing effective protocols for polyploidy induction and for the production of high yielding ploidy mutant [25].…”

Section: Induction Of Polyploidymentioning

confidence: 99%

Studies on Colchicine Induced Chromosome Doubling for Enhancement of Quality Traits in Ornamental Plants

Manzoor

Ahmad

Bashir

et al. 2019

Plants

130

118

View full text Add to dashboard Cite

Polyploidy has the utmost importance in horticulture for the development of new ornamental varieties with desirable morphological traits referring to plant size and vigor, leaf thickness, larger flowers with thicker petals, intense color of leaves and flowers, long lasting flowers, compactness, dwarfness and restored fertility. Polyploidy may occur naturally due to the formation of unreduced gametes or can be artificially induced by doubling the number of chromosomes in somatic cells. Usually, natural polyploid plants are unavailable, so polyploidy is induced synthetically with the help of mitotic inhibitors. Colchicine is a widely used mitotic inhibitor for the induction of polyploidy in plants during their cell division by inhibiting the chromosome segregation. Different plant organs like seeds, apical meristems, flower buds, and roots can be used to induce polyploidy through many application methods such as dipping/soaking, dropping or cotton wool. Flow cytometry and chromosome counting, with an observation of morphological and physiological traits are routine procedures for the determination of ploidy level in plants.

show abstract

Section: Induction Of Polyploidymentioning

confidence: 99%

Studies on Colchicine Induced Chromosome Doubling for Enhancement of Quality Traits in Ornamental Plants

Manzoor

Ahmad

Bashir

et al. 2019

Plants

130

118

View full text Add to dashboard Cite

show abstract

“…Stomatal characters are also known to correlate with the ploidy level (Chen et al 2009;Winarto et al 2010). Large stomatal size is usually associated with higher ploidy levels (Yu et al 2009;Azmi et al 2015;Grosso et al 2017). According to Grosso et al (2017), tetraploid plants have a larger stomatal length than the diploid.…”

Section: Discussionmentioning

confidence: 99%

Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms

et al. 2021

View full text Add to dashboard Cite

Abstract. Putri HA, Purwito A, Sudarsono, Sukma D. 2021. Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms. Biodiversitas 22: 1077-1090. Phalaenopsis amabilis (L.) Blume is a prominent donor for the white petal and sepal trait in Phalaenopsis breeding. However, it has an undesirable character, such as susceptible to soft-rot disease. Therefore, developing soft-rot resistance mutants through gamma irradiation could be explored. This study aimed to evaluate the variability of plantlets regenerated from irradiated and non-irradiated protocorms using morphology, stomatal size and molecular markers and to test responses of the plantlets against soft-rot disease. The plantlets were regenerated from irradiated (5, 10, 15 or 20 Gy) and non-irradiated protocorms. The results showed that P. amabilis plantlet variants were successfully identified based on their leaf morphology and stomatal size variations. A few plantlets have low stomatal densities, large stomatal size, and high chloroplast numbers, which indicated they were polyploids. Leaf disc assay for soft-rot disease response grouped most of the plantlets into very susceptible or susceptible. Moreover, four soft-rot resistant plantlets regenerated from irradiated and non-irradiated protocorms were successfully identified. The resistant plantlets were identified after three consecutive periods of inoculations with pathogens causing soft-rot disease. The evaluation also confirmed nucleotide variation in the Pto gene isolated from different levels of plantlet variant resistance responses.

show abstract

“…This program facilitated a breeding process that feeds the needs of the nursery business. Polyploidization can typically be achieved by introduction of colchicine in many agricultural or horticultural crops including orchids (Griesbach, 1981; Caperta et al, 2006; Azmi et al, 2016; Tuwo and Indrianto, 2016). One of the most successful product series, “Big White Flower,” is comprised of tetraploid Phalaenopsis hybrids developed through polyploidization with the parents Phalaenopsis Doris.…”

Section: Flower Development Of Orchidsmentioning

confidence: 99%

Floral Induction and Flower Development of Orchids

et al. 2019

View full text Add to dashboard Cite

Orchids comprise one of the largest, most highly evolved angiosperm families, and form an extremely peculiar group of plants. Various orchids are available through traditional breeding and micro-propagation since they are valuable as potted plants and/or cut flowers in horticultural markets. The flowering of orchids is generally influenced by environmental signals such as temperature and endogenous developmental programs controlled by genetic factors as is usual in many flowering plant species. The process of floral transition is connected to the flower developmental programs that include floral meristem maintenance and floral organ specification. Thanks to advances in molecular and genetic technologies, the understanding of the molecular mechanisms underlying orchid floral transition and flower developmental processes have been widened, especially in several commercially important orchids such as Phalaenopsis, Dendrobium and Oncidium. In this review, we consolidate recent progress in research on the floral transition and flower development of orchids emphasizing representative genes and genetic networks, and also introduce a few successful cases of manipulation of orchid flowering/flower development through the application of molecular breeding or biotechnology tools.

show abstract

Polyploidy Induction of Moth Orchid (Phalaenopsis amabilis (L.) Blume) by Colchicine Treatment on Pollinated Flowers

Abstract: S The effectiveness of colchicine treatment at inducing polyploidy in Phalaenopsis amabilis

Cited by 12 publications

References 22 publications

Studies on Colchicine Induced Chromosome Doubling for Enhancement of Quality Traits in Ornamental Plants

Studies on Colchicine Induced Chromosome Doubling for Enhancement of Quality Traits in Ornamental Plants

Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms

Floral Induction and Flower Development of Orchids

Contact Info

Product

Resources

About