Polyploidy induction in Physalis alkekengi

Abstract

Physalis alkekengi is an ornamental plant that can also be used as a medicinal plant due to its anti-inflammatory, bactericidal, antitumor and fungicidal properties. Polyploidization can be an important tool in the genetic improvement of this species. The objective this work was to obtain tetraploids in vitro and to evaluate the phytotechnical traits of P. alkekengi. For this, nodal segments of P. alkekengi var. Franchettii were inoculated into petri dishes containing 100 ml of MS medium supplemented with colchicine at concentrations 0; 0.04; 0.08; 0.12; and 0.16% and kept in the dark for 24 and 48h. After the respective treatment periods with colchicine the segments were inoculated into test tubes. The tetraploids were identified by flow cytometry and classical cytogenetics. In vitro seedlings were measured: root length, nodal segment length, leaflet number and total leaf area. In the acclimatization phase, the area of ​​the second leaf and total leaf, petiole radius, stem length, fruit weight with calyx, without calyx, fruit diameter, number of seeds and brix of the pulp were evaluated. Chlorophyll a, chlorophyll b, total chlorophyll, total carotenoid, total chlorophyll / total carotenoid ratio and chlorophyll a / b ratio were also estimated. The treatment that most produced tetraploid seedlings was with 0.08% colchicine per 24h. No significant difference was observed in 7 (seven) variables, these being all variables of photopigments, stem diameter (steam) and brix. In general, diploid (2x) plants were better in 9 (nine) while tetraploid seedlings were better in 6 (six) of the phytotechnical variables. It was concluded that the MS medium supplemented with 0.08% colchicine for 24 h allowed P. alkekengi tetraploides to be obtained with better phytotechnical qualities.

The Effect of Colchicine on Profiles and Contents of Withanolides of Ciplukan (Physalis angulata) In vitro

“…Polyploidization using nodal segments of P. alkekengi var. Franchettii showed that MS medium supplemented with 0.08% colchicine for 24 h resulted tetraploides plant [27]. However, until now no one has reported the effect of colchicine on the profile and content of withanolides especially in Physalis plants.…”

First report of Lema bilineata Germar (Coleoptera: Chrysomelidae) damaging Physalis peruviana (L.) in Brazil

“…The Cape gooseberry, Physalis peruviana L. (Solanaceae), is native to the Andean region and is commercially grown in Brazil (MUNIZ et al, 2014) due to its pharmacological and nutritional benefits (SANTOS et al, 2020). It has also become an attractive alternative for small farmers seeking to diversify their crops because of the high added value of its fruits and its low costs of cultivation (PUENTE et al, 2011).…”