Polyploidization of Indotyphlops braminus, evidence from Isoform-sequencing

Abstract

Indotyphlops braminus is the only known triploid parthenogenetic snake, making it a compelling species to understand the mechanism of emergence of polyploid vertebrates. In this study, we applied Isoform-sequencing technology to sequence full-length transcriptomes of I. braminus. A total of non-redundant 51,849 transcript assemblies of this snake were finally generated and fully annotated with diverse gene function databases. The phylogenetic tree shows that I. braminus and Python bivittatus diverged 98.15 million years ago. One instance of subgenomic divergence in I. braminus was identified, dating at 11.5 ~ 15 million years ago. The full-length transcriptome resource generated as part of this research will aid transcriptome analysis and genomic evolution studies in the future.