Polyphyllin VII suppresses cell proliferation, the cell cycle and cell migration in colorectal cancer

Song, Cheng; Pan, Bo; Yang, Xiao; Tang, Wei

doi:10.3892/ol.2020.12286

Cited by 9 publications

(6 citation statements)

References 47 publications

(56 reference statements)

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“…In addition, polyphyllin VII significantly inhibited the migration and invasion of CTC-TJH-01 and H1975 cells in vitro and significantly downregulated the expression of MMP-2 and MMP-9 proteins in CTC-TJH-01 and H1975 cells. These observations are consistent with previous studies demonstrating the inhibitory effect of polyphyllin VII on the migration of HCT116 and HepG2 cells in vitro 12 , 13 .…”

Section: Discussionsupporting

confidence: 93%

“…Polyphyllin VII, a steroidal saponin derived from Paris polyphylla Sm., possesses potent anticancer activity against various human cancer cell lines 13 , 14 . Previous studies have reported the inhibitory effects of polyphyllin VII on the proliferation and apoptosis induction of different types of cancer cells, including human lung cancer cells (A549 and NCI-H1299 cells), human oral cancer cells (SAS and OECM-1 cells), human liver cancer cells (HepG2 cells), human nasopharyngeal cancer cells (HONE-1 and NPC-039 cells) and human colorectal cancer cells (HCT-116 and HT-29 cells) 12 - 14 , 25 , 26 . In our study, we utilized our own established human lung cancer cell line CTC-TJH-01, which possesses metastatic capabilities, as well as the commercial lung cancer cell line H1975, to investigate the anticancer efficacy of polyphyllin VII.…”

Section: Discussionmentioning

confidence: 99%

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Polyphyllin VII induces CTC anoikis to inhibit lung cancer metastasis through EGFR pathway regulation

Que,

Luo,

et al. 2023

Int. J. Biol. Sci.

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Circulating tumor cells (CTCs) are cells that detach from the primary tumor and enter the bloodstream, playing a crucial role in the metastasis of lung cancer. Unfortunately, there is currently a lack of drugs specifically designed to target CTCs and prevent tumor metastasis. In this study, we present evidence that polyphyllin VII, a potent anticancer compound, effectively inhibits the metastasis of lung cancer by inducing a process called anoikis in CTCs. We observed that polyphyllin VII had significant cytotoxicity and inhibited colony formation, migration, and invasion in both our newly established cell line CTC-TJH-01 and a commercial lung cancer cell line H1975. Furthermore, we found that polyphyllin VII induced anoikis and downregulated the TrkB and EGFR-MEK/ERK signaling pathways. Moreover, activation of TrkB protein did not reverse the inhibitory effect of polyphyllin VII on CTCs, while upregulation of EGFR protein effectively reversed it. Furthermore, our immunodeficient mouse models recapitulated that polyphyllin VII inhibited lung metastasis, which was associated with downregulation of the EGFR protein, and reduced the number of CTCs disseminated into the lungs by inducing anoikis. Together, these results suggest that polyphyllin VII may be a promising compound for the treatment of lung cancer metastasis by targeting CTCs.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Polyphyllin VII induces CTC anoikis to inhibit lung cancer metastasis through EGFR pathway regulation

Que,

Luo,

et al. 2023

Int. J. Biol. Sci.

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“…The PI3K-AKT signaling pathway and adaptive immune response pathway were activated in low-risk patients. It is known that the abnormal regulation of the cell cycle and DNA replication played a crucial role in promoting tumor growth ( Song et al, 2021 ). The previous study showed that constitutive activation of NF-κB was characteristic of most ABC subtypes of DLBCL, and the activation of the NF-κB pathway may be one of the mechanisms resulting in drug resistance of relapsed/refractory DLBCL ( Turturro, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

An Autophagy-Related Gene Signature can Better Predict Prognosis and Resistance in Diffuse Large B-Cell Lymphoma

Zhou

He²,

Liú³

et al. 2022

Front. Genet.

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Background: Diffuse large B-cell lymphoma (DLBCL) is a highly heterogeneous disease, and about 30%–40% of patients will develop relapsed/refractory DLBCL. In this study, we aimed to develop a gene signature to predict survival outcomes of DLBCL patients based on the autophagy-related genes (ARGs).Methods: We sequentially used the univariate, least absolute shrinkage and selector operation (LASSO), and multivariate Cox regression analyses to build a gene signature. The Kaplan–Meier curve and the area under the receiver operating characteristic curve (AUC) were performed to estimate the prognostic capability of the gene signature. GSEA analysis, ESTIMATE and ssGSEA algorithms, and one-class logistic regression were performed to analyze differences in pathways, immune response, and tumor stemness between the high- and low-risk groups.Results: Both in the training cohort and validation cohorts, high-risk patients had inferior overall survival compared with low-risk patients. The nomogram consisted of the autophagy-related gene signature, and clinical factors had better discrimination of survival outcomes, and it also had a favorable consistency between the predicted and actual survival. GSEA analysis found that patients in the high-risk group were associated with the activation of doxorubicin resistance, NF-κB, cell cycle, and DNA replication pathways. The results of ESTIMATE, ssGSEA, and mRNAsi showed that the high-risk group exhibited lower immune cell infiltration and immune activation responses and had higher similarity to cancer stem cells.Conclusion: We proposed a novel and reliable autophagy-related gene signature that was capable of predicting the survival and resistance of patients with DLBCL and could guide individualized treatment in future.

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“…It exerts anticancer activities by the suppression of proliferation, cell cycle arrest, and modulation of drugs (He et al, 2016; Lin et al, 2015; Zhang, Jia, Bao, et al, 2016; Zhang, Jia, Wang, et al, 2016). It has been demonstrated that polyphyllin VII showed a potent effect on colorectal cancer (Song et al, 2021), multiple myeloma (Wang et al, 2022), ovarian cancer (Zhao et al, 2021), oral cancer (Hsieh et al, 2016), hepatoblastoma cancer, and lung cancer cells (Chen et al, 2016). Besides the anticancer effect, polyphyllin VII has been found to have anti‐inflammatory activity (Zhang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

A simple and sensitive LC–MS/MS method for the determination of polyphyllin VII in rat plasma and its application to pharmacokinetic study

Wang

Han

et al. 2023

Biomedical Chromatography

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Polyphyllin VII is an isoprene saponin extracted from Rhizoma paridis, and it can effectively suppress tumor initiation, growth, and metastasis. In this study, we aim to develop and validate an LC-MS/MS method for the quantification of polyphyllin VII in rat plasma using digoxin as the internal standard (IS). The plasma samples were precipitated with methanol, and the samples were separated on an ACQUITY BEH C 18 column (2.1 Â 50 mm, 1.7 μm). The mobile phase consisted of 0.1% formic acid solution and acetonitrile. The detection was performed in the multiple reactions monitoring mode, with the precursor-to-product transitions of m/z 1075.4 > 883.3 for polyphyllin VII and m/z 779.4 > 649.6 for the IS. The method showed excellent linearity over the concentration range of 0.5-1000 ng/ml, with a correlation coefficient of 0.9996 (r = 0.9996). The lower limit of quantification was 0.5 ng/ml. The inter-and intra-day accuracy (relative error) ranged from À4.8 to 5.9%, and precision (relative standard deviation) was < 9.0%. The assay showed high extraction recovery, ranging from 90.6 to 95.6%. Polyphyllin VII was demonstrated to be stable under the storage conditions. The validated LC-MS/MS method was successfully applied to the pharmacokinetic study of polyphyllin VII in rats after oral, intraperitoneal, and intravenous administrations. The pharmacokinetic results indicated that polyphyllin VII showed low oral (5.86%) bioavailability and moderate (38.81%) intraperitoneal bioavailability.

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Polyphyllin VII suppresses cell proliferation, the cell cycle and cell migration in colorectal cancer

Cited by 9 publications

References 47 publications

Polyphyllin VII induces CTC anoikis to inhibit lung cancer metastasis through EGFR pathway regulation

Polyphyllin VII induces CTC anoikis to inhibit lung cancer metastasis through EGFR pathway regulation

An Autophagy-Related Gene Signature can Better Predict Prognosis and Resistance in Diffuse Large B-Cell Lymphoma

A simple and sensitive LC–MS/MS method for the determination of polyphyllin VII in rat plasma and its application to pharmacokinetic study

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