Polyphyllin I suppresses human osteosarcoma growth by inactivation of Wnt/β-catenin pathway in vitro and in vivo

Chang, Junli; Li, Yimian; Wang, Xianyang; Hu, Shaopu; Wang, Hongshen; Shi, Qi; Wang, Yongjun; Yang, Yanping

doi:10.1038/s41598-017-07194-9

Cited by 41 publications

(39 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The compound was found to suppress in vitro growth of osteosarcoma 143-B and HOS cells, as well as the primary cells from a osteosarcoma patient and, what is more important, inhibited in vivo intratibial primary tumor growth in xenograft orthotopic mouse model. Moreover, it induced cell apoptosis, cell cycle arrest and inhibited the invasion and migration of osteosarcoma cells (Chang et al 2017). Other interesting effects were obtained in the studies on concomitant administration of PPI with other compounds, including currently used chemotherapeutics.…”

Section: Results Of In Vivo Studiesmentioning

confidence: 93%

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

et al. 2020

View full text Add to dashboard Cite

Steroidal saponins are a group of glycosides widely distributed among monocotyledonous families. They exert a wide spectrum of biological effects including cytotoxic and antitumor properties which are the most studied. This review is an update of our previous paper-Saponins as cytotoxic agents (Podolak et al. in Phytochem Rev 9:425-474, 2010) and covers studies that were since published (2010-2018). In this paper we refer to steroidal saponins presenting results of cytotoxicity studies, mechanisms of action and structure-activity relationships.

show abstract

Section: Results Of In Vivo Studiesmentioning

confidence: 93%

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Previous studies, including ours, showed that PPI inhibited growth and induced cell cycle arrest, and apoptosis of human cancer cells [12,14,15]. In the current study, we examined the potential synergy of the combination of PPI and enzalutamide in this process.…”

Section: Combination Of Ppi and Enzalutamide Further Inhibited Growthmentioning

confidence: 99%

“…PPI also demonstrated anti-tumor activity on ovarian cancer in vivo via modulation of caspase-9, c-Jun, and secreted glycoprotein Wnt5a [13]. Moreover, PPI suppressed growth, induced apoptosis, and inhibited the invasion and migration of osteosarcoma cells by inactivating the Wnt/β-catenin pathway both in vitro and in vivo [14]. We previously showed that PPI inhibited the growth of human lung cancer cells via stress-activated protein kinase/c-Jun N-terminal kinase-mediated inhibition of the protein expression of the nuclear factor-kappaB (NF-κB) subunit p65 and DNA methyltransferase 1.…”

Section: Introductionmentioning

confidence: 98%

Crosstalk of NF-κB/P65 and LncRNA HOTAIR-Mediated Repression of MUC1 Expression Contribute to Synergistic Inhibition of Castration-Resistant Prostate Cancer by Polyphyllin 1–Enzalutamide Combination Treatment

Xiang

Zou

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Polyphyllin I (PPI), one of the steroidal saponins in Paris polyphylla, reportedly exhibits antitumor effects. However, the detailed mechanism underlying PPI, particularly in enhancing the effect of the androgen receptor inhibitor enzalutamide in controlling castration-resistant prostate cancer (CRPC) has not been explored. Methods: Cell viability and cell cycle distribution were measured using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and flow cytometry assays, respectively. Long non-coding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) expression was measured by quantitative real time-PCR (qRT-PCR). Western blot analysis was performed to determine the protein expression levels of MUC1, p65, and p50. Silencing of HOTAIR was evaluated using the siRNA procedure. The promoter activity of the MUC1 gene was determined using Secrete-Pair Dual Luminescence Assay Kit. Exogenous expression of HOTAIR, p65, and MUC1 was conducted by transient transfection assay. A xenograft tumor model in nude mice was used to further evaluate the effect of the combination of PPI and enzalutamide in vivo. Results: We showed that PPI significantly inhibited growth and induced cell cycle arrest in CRPC cells. PPI also decreased p65 and MUC1 protein expression and reduced HOTAIR expression. Exogenously expressed p65 resisted the PPI-inhibited expression of HOTAIR, whereas silenced HOTAIR reduced MUC1 protein but exerted no effect on the expression of p65 and p50 proteins. Conversely, exogenously expressed HOTAIR resisted the PPI-inhibited MUC1 protein expression, and excessive expression of MUC1 antagonized the PPI-inhibited cell growth. Notably, PPI combined with enzalutamide exerted a synergistic effect. Consistent with this finding, PPI inhibited tumor growth, HOTAIR expression, as well as p65 and MUC1 protein expressions in vivo. Conclusions: Our results indicate that PPI inhibits the growth of CRPC cells by inhibiting p65 protein and concomitantly reducing HOTAIR expression, thereby suppressing MUC1 gene expression. The novel regulatory interaction of p65 and HOTAIR converge in the inhibition of MUC1 expression and overall PPI response. The combination of PPI and enzalutamide exhibits synergy. This study reveals a novel mechanism underlying the synergistic inhibitory effect of PPI and enzalutamide on the growth of CRPC cells.

show abstract

“…However, approximately 40% of OS patients have been found to have metastasized at the time of initial diagnosis, which poses a serious challenge to patient prognosis and long-term survival. 2 Therefore, early diagnosis and treatment of OS is critical to improving patient outcomes.…”

Section: Introductionmentioning

confidence: 99%

<p>lncRNA FLVCR-AS1 promotes osteosarcoma growth by targeting miR381-3p/CCND1</p>

Yang¹,

He²,

Duan³

et al. 2020

OTT

View full text Add to dashboard Cite

Purpose: This article reports on FLVCR-AS1 effects on osteosarcoma (OS) growth. Methods: Tumor tissue and adjacent normal tissue of 48 OS patients were collected. HOS and 143B cells were transfected. Gene expression was examined with qRT-PCR and Western blot. CCK8 assays and cell cloning was performed to measure cell proliferation. Cell cycle and apoptosis were assessed. Luciferase-reporter gene assays and RNA pull-down tests were used to detect targeting relationships between genes. Results: Prominently higher FLVCR-AS1 expression was found in OS tissue and cells, and was associated with poor prognosis (P<0.05, P<0.01, or P<0.001). Compared with the siCtrl group, 143B and HOS cells of the siFLVCR-AS1 group had significantly lower OD 450 values and clone numbers and obviously higher percentages of cells in the G 1 phase and apoptosis (P<0.01 or P<0.001). miR381-3p expression was directly inhibited by FLVCR-AS1, and CCND1 expression was directly suppressed by miR381-3p. Compared with the FLVCR-AS1 group, 143B cells of the FLVCR-AS1 + miR381-3p mimic group and FLVCR-AS1 + siCCND1 group showed remarkably lower OD 450 values and clone numbers obviously higher apoptosis and percentage of cells in the G 1 phase (P<0.05, P<0.01, or P<0.001). Conclusion: FLVCR-AS1 promoted OS growth by upregulating CCND1 expression via downregulation of miR381-3p.

show abstract

Polyphyllin I suppresses human osteosarcoma growth by inactivation of Wnt/β-catenin pathway in vitro and in vivo

Cited by 41 publications

References 30 publications

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

Crosstalk of NF-κB/P65 and LncRNA HOTAIR-Mediated Repression of MUC1 Expression Contribute to Synergistic Inhibition of Castration-Resistant Prostate Cancer by Polyphyllin 1–Enzalutamide Combination Treatment

<p>lncRNA FLVCR-AS1 promotes osteosarcoma growth by targeting miR381-3p/CCND1</p>

Contact Info

Product

Resources

About