Polyphyllin I inhibits proliferation and metastasis of ovarian cancer cell line HO-8910PM in vitro

Gu, Linhui; Feng, Jianguo; Xu, Haiyan; Luo, Min; Su, Dan

doi:10.1016/s0254-6272(13)60174-0

Cited by 33 publications

(24 citation statements)

References 9 publications

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“…The in vitro migration assays were performed as previously described with some modifications (18,19) using Transwells (8-µm pore size; BD Corporation, Franklin Lakes, NJ, USA). The COLO205 cells were added to the upper inserts of the chamber (200 µl serumfree medium containing 2x10 4 cells), and 600 µl medium with 1% FBS was added to the lower well.…”

Section: Cucurbitacin I Inhibits Cell Migration and Invasion And Enhamentioning

confidence: 99%

Cucurbitacin I inhibits cell migration and invasion and enhances chemosensitivity in colon cancer

Song

Liu

et al. 2015

Oncology Reports

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Abstract. Colorectal cancers are the third most common types of cancers worldwide. Surgical resection is unable to eliminate tumors completely due to metastasis. A demand for new chemotherapeutic tools exists. In the present study, we examined the chemopreventive potential of cucurbitacin I, a natural component extracted from plants of the Cucurbitaceae family, in the colon cancer cell line COLO205. We hypothesized that cucurbitacin I would prevent colon cancer cell migration and invasion, and sensitize colon cancer cells to chemotherapy. Our data demonstrated that exposure of the COLO205 cells to cucurbitacin I significantly decreased cell viability. Furthermore our data demonstrated for the first time that in the COLO205 cells, cucurbitacin I could suppress the cell migration and invasion, and harbor chemosensitization activity against colon cancer. The anticancer activity of cucurbitacin I was accomplished by downregulating p-STAT3 and MMP-9 expression. Collectively, our results suggest that cucurbitacin I may be a potent adjuvant chemotherapeutic agent for colon cancer with anti-migration, anti-invasion and chemosensitizing activities.

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Section: Cucurbitacin I Inhibits Cell Migration and Invasion And Enhamentioning

confidence: 99%

Cucurbitacin I inhibits cell migration and invasion and enhances chemosensitivity in colon cancer

Song

Liu

et al. 2015

Oncology Reports

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“…In human lung cancer cell lines A549, SPCA-1, H1975, NCI-H446, NCI-H460, NCI-H292 and NCI-H69, periplocin can inhibit proliferation and induce apoptosis of cancer cells by the Akt and ERK signaling pathway in vitro and in vivo (94). Polyphyllin I, a component derived from Rhizoma Paridis Chonglou, was proved to suppress proliferation and metastasis of human ovarian cancer cell line HO-8910PM by activating the JNK signaling pathway (95).…”

Section: Saponinmentioning

confidence: 99%

A map describing the association between effective components of traditional Chinese medicine and signaling pathways in cancer cells in vitro and in vivo

Xia

Chen

Zhang

et al. 2014

DD^|^T

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“…Polyphyllin I (PPI), an active component obtained from Rhizoma Paridis saponins, has been reported to exhibit various biological activities against many types of cancer [9][10][11][12]. In one study, PPI inhibited tumor growth and induced apoptosis in human breast cancer cells, and these effects were enhanced by activating the PTEN-induced kinase 1 signaling pathway [11].…”

Section: Introductionmentioning

confidence: 99%

Crosstalk of NF-κB/P65 and LncRNA HOTAIR-Mediated Repression of MUC1 Expression Contribute to Synergistic Inhibition of Castration-Resistant Prostate Cancer by Polyphyllin 1–Enzalutamide Combination Treatment

Xiang

Zou

et al. 2018

Cell Physiol Biochem

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Background/Aims: Polyphyllin I (PPI), one of the steroidal saponins in Paris polyphylla, reportedly exhibits antitumor effects. However, the detailed mechanism underlying PPI, particularly in enhancing the effect of the androgen receptor inhibitor enzalutamide in controlling castration-resistant prostate cancer (CRPC) has not been explored. Methods: Cell viability and cell cycle distribution were measured using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and flow cytometry assays, respectively. Long non-coding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) expression was measured by quantitative real time-PCR (qRT-PCR). Western blot analysis was performed to determine the protein expression levels of MUC1, p65, and p50. Silencing of HOTAIR was evaluated using the siRNA procedure. The promoter activity of the MUC1 gene was determined using Secrete-Pair Dual Luminescence Assay Kit. Exogenous expression of HOTAIR, p65, and MUC1 was conducted by transient transfection assay. A xenograft tumor model in nude mice was used to further evaluate the effect of the combination of PPI and enzalutamide in vivo. Results: We showed that PPI significantly inhibited growth and induced cell cycle arrest in CRPC cells. PPI also decreased p65 and MUC1 protein expression and reduced HOTAIR expression. Exogenously expressed p65 resisted the PPI-inhibited expression of HOTAIR, whereas silenced HOTAIR reduced MUC1 protein but exerted no effect on the expression of p65 and p50 proteins. Conversely, exogenously expressed HOTAIR resisted the PPI-inhibited MUC1 protein expression, and excessive expression of MUC1 antagonized the PPI-inhibited cell growth. Notably, PPI combined with enzalutamide exerted a synergistic effect. Consistent with this finding, PPI inhibited tumor growth, HOTAIR expression, as well as p65 and MUC1 protein expressions in vivo. Conclusions: Our results indicate that PPI inhibits the growth of CRPC cells by inhibiting p65 protein and concomitantly reducing HOTAIR expression, thereby suppressing MUC1 gene expression. The novel regulatory interaction of p65 and HOTAIR converge in the inhibition of MUC1 expression and overall PPI response. The combination of PPI and enzalutamide exhibits synergy. This study reveals a novel mechanism underlying the synergistic inhibitory effect of PPI and enzalutamide on the growth of CRPC cells.

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Polyphyllin I inhibits proliferation and metastasis of ovarian cancer cell line HO-8910PM in vitro

Cited by 33 publications

References 9 publications

Cucurbitacin I inhibits cell migration and invasion and enhances chemosensitivity in colon cancer

Cucurbitacin I inhibits cell migration and invasion and enhances chemosensitivity in colon cancer

A map describing the association between effective components of traditional Chinese medicine and signaling pathways in cancer cells in vitro and in vivo

Crosstalk of NF-κB/P65 and LncRNA HOTAIR-Mediated Repression of MUC1 Expression Contribute to Synergistic Inhibition of Castration-Resistant Prostate Cancer by Polyphyllin 1–Enzalutamide Combination Treatment

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