Polyphenols of Cassia tora leaves prevents lenticular apoptosis and modulates cataract pathology in Sprague-Dawley rat pups

Vasudevan, Sreelakshmi; Abraham, Annie

doi:10.1016/j.biopha.2016.04.018

Cited by 15 publications

(7 citation statements)

References 33 publications

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“…PCR reactions included 5 min enzyme activation at 95°C, followed by 40 cycles with 95°C for 5 s (denaturing) and 60°C for 10 s (annealing/extension). The changes in target genes expression were estimated using comparative CT (∆∆CT, threshold cycle) method and presented as relative quantification using the 2 -∆∆CT formula (Sreelakshmi & Abraham, 2016).…”

Section: Quantitative Analysis Of Apoptosis-related Gene Bax and Bcmentioning

confidence: 99%

Esculetin and idebenone ameliorate galactose‐induced cataract in a rat model

et al. 2020

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Cataract is the principal cause of blindness. The enzyme, aldose reductase (AR) is a key player in polyol pathway. Buildup of polyols and oxidative stress are the main causes of cataractogenesis. This study investigated the cytoprotective properties of esculetin and idebenone in galactose‐induced cataract. Rats were partitioned into four groups each of ten rats. Control group was fed with normal diet; group 2 rats were fed with galactose diet (50%); groups 3, 4 rats were fed with galactose diet concurrently with either esculetin (50 mg/kg BW) or idebenone (100 mg/kg BW), for 20 days. The study revealed that esculetin and idebenone significantly reduced the elevated levels of Bax/Bcl‐2 ratio, malondialdehyde, and DNA fragmentation and increased total antioxidant capacity level in lenses compared to the cataract‐induced group. Only esculetin decreased AR, galactitol, and advanced glycated end products levels in lenses. Histopathological examinations supported the biochemical findings. Esculetin and idebenone may have chemopreventive effects for sugar cataract. Practical applications Cataract is an age‐related disease that might cause blindness in older adult people. Presently, no absolute pharmacological treatment is accessible for cataract. The use of natural products or their derivatives attract particular attention in modern medicines as they are believed to be safer with few or no side effects. Esculetin is a polyphenolic compound found in many medicinal plants. Idebenone is a synthetic analogue of coenzyme Q10. The current study is an approach to explore the anticataract effects of esculetin and idebenone in galactose‐induced cataract in rats. Our study proved that both agents have anticataractogenic potentials due to their antioxidant and antiapoptotic properties.

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Section: Quantitative Analysis Of Apoptosis-related Gene Bax and Bcmentioning

confidence: 99%

Esculetin and idebenone ameliorate galactose‐induced cataract in a rat model

et al. 2020

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“…This results in lens epithelial cell apoptosis and connexin modifications, the hall mark of cataractogenesis [16]. We previously reported the preventive potential of an edible plant Cassia tora in alleviating oxidative stress induced cataract on neonatal rats, and identified a flavonoid (kaempferol) and two anthraquinones (chrysophanol and emodin) from its leaves [17][18][19].…”

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confidence: 99%

Evaluation of the Drug-like Properties of Kaempferol, Chrysophanol and Emodin and their Interactions with EGFR Tyrosine Kinase - An in silico Approach

Vasudevan

Raj

Abraham

2017

Natural Product Communications

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In this study, a molecular docking was performed on EGFR tyrosine kinase with plant phenolic compounds kaempferol, chrysophanol and emodin; identified from Cassia tora, an edible plant employed for eye diseases traditionally. The results illustrated that all the compounds have strong binding abilities with epidermal growth factor receptor and validated the reported anticataractogenic potential of C. tora leaves. Further, the compounds also satisfied the criteria for being a drug through its structural features. Taken together, it was proposed that the compounds; kaempferol, chrysophanol and emodin might be helpful for further drug design and development and could be employed as efficient lead compounds in ophthalmic drug formulations.

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“…The identification of compounds by ESI-MS is based on the comparison with standards from a library of plant phenolics (Figures S1 and S2 ) (Sreelakshmi & Abraham 2016b ). The present work is an extension of our previous studies on the attenuation of cataract pathology by reducing protein and lipid modifications by Cassia tora leaves on oxidative stress cataract models (Sreelakshmi & Abraham 2016a , 2016b , 2016c ). The current study is designed to evaluate the mechanism of anti-cataractogenic effect of edible Cassia tora leaves on selenite-induced animal models by focusing on membrane integrity, energy metabolism, proteolysis and ubiquitin-proteasome pathway in the lens.…”

Section: Introductionmentioning

confidence: 71%

Protective effects of Cassia tora leaves in experimental cataract by modulating intracellular communication, membrane co-transporters, energy metabolism and the ubiquitin-proteasome pathway

Sreelakshmi

Abraham

2017

Pharmaceutical Biology

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Context: Cataract is the clouding of eye lens which causes impairment in vision and accounts for the leading factor of global blindness. Functional food-based prevention of cataract finds application in vision research because of its availability and easy access to all classes of the society. Cassia tora Linn. (Caesalpinaceae) is an edible plant mentioned in the traditional systems of medicine for whole body health, especially to the eyes.Objective: The present study evaluates the potential of ethyl acetate fraction of Cassia tora leaves (ECT) on experimental cataract.Materials and methods: Cataract was induced by a single subcutaneous injection of sodium selenite (4 μg/g body weight) on 10th day. ECT was supplemented orally from 8th day up to 12th day at a concentration of 5 μg/g body weight and marker parameters were evaluated after 30 days.Results: The production of MPO and the activation of calpain were reduced 52.17% and 36.67% by ECT in lens tissue, respectively. It modulated the energy status by significantly increasing the activity of CCO 1 (55.56%) and ATP production (41.88%). ECT maintained the ionic balance in the lens by reducing the level of sodium (50%) and increasing the level of potassium (42.5%). It also reduced cell junction modifications and preserved a functional ubiquitin-proteasome pathway.Discussion and conclusion: The results reinforce the growing attention on wild plant food resources for preventive protection against cataract. The data suggest the value of Cassia tora leaves as a functional food for ameliorating cataract pathology.

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Polyphenols of Cassia tora leaves prevents lenticular apoptosis and modulates cataract pathology in Sprague-Dawley rat pups

Cited by 15 publications

References 33 publications

Esculetin and idebenone ameliorate galactose‐induced cataract in a rat model

Esculetin and idebenone ameliorate galactose‐induced cataract in a rat model

Evaluation of the Drug-like Properties of Kaempferol, Chrysophanol and Emodin and their Interactions with EGFR Tyrosine Kinase - An in silico Approach

Protective effects of Cassia tora leaves in experimental cataract by modulating intracellular communication, membrane co-transporters, energy metabolism and the ubiquitin-proteasome pathway

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