Polyphenols in the leaves of some eucalyptus species

Hillis, W. E.; Hingston, F. J.

doi:10.1002/jsfa.2740141204

Cited by 16 publications

(2 citation statements)

References 20 publications

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“…In E. sieberi, three flavanones (alpinetin, pinocembrin and O,O-dimethylpinocembrin) have been identified (Bick et al, 1972). The most frequent flavonol aglycone found in Eucalyptus leaves is quercetin (Elkeiy et al, 1966;Hills, 1966;Boukef et al, 1976) and the most abundant glycosides are the 3-glucosides, 3-rhamnosides, 3-rutinosides and 3-glucuronides of quercetin (Hillis and Hingston, 1963;Hillis and Isoi, 1965;Elkeiy et al, 1966;Okamura et al, 1993), kaempferol (Hillis and Isoi, 1965;Abd-Alla et al, 1980;Okamura et al, 1993) and miricetin (Hillis and Hingston, 1963;Abd-Alla et al, 1980). Aromadendrin (dihydrokaempferol) has also been identified (Hillis and Isoi, 1965;Bick et al, 1972).…”

Section: Introductionmentioning

confidence: 99%

Low molecular weight polyphenols in leaves of Eucalyptus camaldulensis, E. globulus and E. rudis

Conde¹,

Cadahía²,

García‐Vallejo³

1997

Phytochem. Anal.

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Ether soluble polyphenols from leaves of Eucalyptus camaldulensis, E. globulus and E. rudis have been studied. The phenolic aldehyde vanillin, the phenolic acids gallic, protocatechuic and ellagic acids, some flavonol glycosides (rutin, quercetin-3-arabinoside, quercetin-3,7-dirhamnoside, quercitrin, kaempferol-3-arabinoside and their aglycones), one flavanone (naringenin) and two flavones (luteolin and apigenin) were identified and quantified. Some ellagitannins, flavonols and flavanones were recognized according to their ultra-violet spectra. E. camaldulensis and E. rudis showed the highest concentrations and varieties of flavonol glycosides, whilst E. globulus was characterized by high concentrations of ellagitannins.

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Section: Introductionmentioning

confidence: 99%

Low molecular weight polyphenols in leaves of Eucalyptus camaldulensis, E. globulus and E. rudis

Conde¹,

Cadahía²,

García‐Vallejo³

1997

Phytochem. Anal.

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“…Eucalyptus leaves contain a wide variety of polyphenolic compounds according to the published flavonoid composition (Hillis and Hingston, 1963;Horn et al, 1964;Lamberton, 1964;Hillis and Isoi, 1965;Elkeiy et al, 1966;Hillis, 1966;Cerecer et al, 1974;Boukef et al, 1976;Abd-Alla et al, 1980;Wollenweber and Kohorst, 1981;Courtney et al, 1983;Okamura et al, 1993a,b). However, there are only a few studies concerning the tannin fraction.…”

Section: Introductionmentioning

confidence: 99%

High Pressure Liquid Chromatographic Analysis of Polyphenols in Leaves of Eucalyptus camaldulensis, E. globulus and E. rudis: Proanthocyanidins, Ellagitannins and Flavonol Glycosides

Cadahía¹,

Conde²,

García‐Vallejo³

et al. 1997

Phytochem. Anal.

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Polyphenols of adult leaves from Eucalyptus globulus, E. camaldulensis and E. rudis grown in Spain, in two areas with different edaphoclimatical characteristics, were estimated by chemical methods and by high pressure liquid chromatography. Leaf extracts consisted mainly of proanthocyanidins, ellagitannins and flavonol glycosides. Knowledge of the ellagitannins allows the discrimination of the three species, with E. globulus being well differentiated from the other two. However, the pattern of flavonol glycosides only permitted the discrimination of E. rudis with respect to E. camaldulensis and E. globulus. EXPERIMENTALSamples. Adult leaves of E. camaldulensis, E. globulus and E. rudis were collected in two Spanish arboreta. The climate at one of the sites, located in the South Western province of Huelva, is warmer and drier than that of the other, which is in the North Eastern province of Pontevedra. Five samples, each from a different tree, were collected for each species at each geographical origin, with the exception of E. camaldulensis from Pontevedra where only two trees were sampled.Extraction. Tannins were extracted from dried and ground leaf samples (10 g) with 300 mL methanol:water (80:20), at room temperature in the dark for 24 h (Conde et al., 1995). The suspensions were filtered, the methanol was removed by vacuum distillation and the aqueous solution was extracted with ethyl ether and freeze dried. The aqueous solution was used for the quantitative analysis of total phenols, proanthocyanidins and ellagitannins, whilst the lyophilized material was used for HPLC analysis after redissolving in methanol:water (1:1).Total phenol content. This was determined by the Folin-Ciocalteu method (Singleton and Rossi, 1965) using aqueous solutions of gallic acid (2-40 g/mL) as standard. The molar absorbance obtained in our analysis conditions was 22.3ϫ 10 3 /mol/cm. Proanthocyanidin content. The evaluation of proanthocyanidins was carried out using the vanillin method (Swain and Hillis, 1959). Calibration was achieved with aqueous solutions of catechin (2-40 g/mL) and a molar absorbance of 34.3ϫ 10 3 /mol/cm was obtained under our conditions. Ellagitannin content. Ellagitannins were estimated by the HPLC evaluation of the ellagic acid obtained following acid hydrolysis.

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Flavanones and Dihydroflavonols

Bohm¹

1975

The Flavonoids

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Polyphenols in the leaves of some eucalyptus species

Cited by 16 publications

References 20 publications

Low molecular weight polyphenols in leaves of Eucalyptus camaldulensis, E. globulus and E. rudis

Low molecular weight polyphenols in leaves of Eucalyptus camaldulensis, E. globulus and E. rudis

High Pressure Liquid Chromatographic Analysis of Polyphenols in Leaves of Eucalyptus camaldulensis, E. globulus and E. rudis: Proanthocyanidins, Ellagitannins and Flavonol Glycosides

Flavanones and Dihydroflavonols

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